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The distribution and origin of the ipsilateral descending limb of the brachium conjunctivum. An autoradiographic and horseradish peroxidase study in the rat

Summary

The distribution, organization and origin of the ipsilateral descending limb of the Brachium Conjunctivum (B.C.), have been studied in the rat by using anterograde and retrograde tracing techniques. After injections of tritiated leucine/proline into the lateral cerebellar nucleus, covering both its medial part, corresponding to the dorsolateral hump (DLH) of Goodman et al. (1963) and its lateral part, (designated here as the lateral dentate, LD), and the neighboring interposed nucleus (NI), emerging fibres are numerous and leave laterally from the B.C. On the contrary, injections restricted to LD reveal very few such fibers. Within the lateral parvocellular reticular formation (LPRF) terminal labelling is heavy, and moderate to sparse within the adjacent trigeminal complex. Rostro-caudally, silver grain accumulation within the LPRF extends from the level of the motor trigeminal nucleus (VM) to the pyramidal decussation, exhibiting a cephalocaudal decrease of grain density. Within the trigeminal complex, labelling occurs in the caudal VM, the dorsal portion of the principal sensory nucleus, and within and around the trigeminal spinalis oralis. In addition, the area surrounding the VM (in part corresponding to the supratrigeminal region of Lorente de Nó 1922, 1933) is moderately labelled. After injections of HRP into various levels of the ipsilateral descending B.C.'s projection field, retrogradely labelled cells are numerous within the DLH. A slightly lesser amount of labelled cells are found in the lateral half of the NI, primarily concerning the nucleus interpositus posterior. Within the LD, only a few labelled cells are observed: these are mainly restricted to the dorsal portion at rostral levels of the nucleus. The results obtained by both the anterograde and retrograde studies suggest an absence of a topographic organization within this descending B.C. component. The possible functional meaning of these results is discussed.

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Abbreviations

B.C.:

Brachium conjunctivum

DLH:

Dorsolateral hump

FTN:

Fibers of the trigeminal nerve

IO:

Inferior olive

LD:

Lateral dentate

LPRF:

Lateral parvocellular reticular formation

NI:

Interposed nucleus

NIA:

Nucleus interpositus anterior

NIP:

Nucleus interpositus posterior

NVII:

Facial nerve

PD:

Pyramidal decussation

TB:

Trapezoid body

VM:

Trigeminal motor nucleus

VPS:

Trigeminal principal sensory nucleus

VSC:

Trigeminal spinalis caudalis subnucleus

VSI:

Trigeminal spinalis interpolaris subnucleus

VSO:

Trigeminal spinalis oralis subnucleus

VII:

Facial nucleus

XII:

Hypoglossal nucleus

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Correspondence to W. Woodson.

Additional information

This work was submitted as partial fulfillment for the degree of Doctorat de 3ème cycle at the Université Pierre et Marie Curie

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Woodson, W., Angaut, P. The distribution and origin of the ipsilateral descending limb of the brachium conjunctivum. An autoradiographic and horseradish peroxidase study in the rat. Exp Brain Res 56, 167–182 (1984). https://doi.org/10.1007/BF00237454

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Key words

  • Lateral
  • Interposed nuclei
  • Ipsilateral descending B.C.
  • Lateral parvocellular reticular formation
  • Trigeminal complex
  • Rat