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Development of haploid asparagus embryos from liquid cultures of anther-derived calli is enhanced by ancymidol

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The effect of ancymidol concentration on the development of haploid asparagus embryos was determined. Liquid cultures from anther-derived calli were grown for three weeks in MS medium plus 1.0 mg l−1 2,4-D, 0.1 mg l−1 NAA, 0.2 mg l−1 kinetin, 800 mg l−1 glutamine, 500 mg l−1 casein hydrolysate, 2% sucrose and 0.0–1.0 mg l−1 ancymidol. Cell clumps (224–500 μm) were plated on solid embryo maturation medium (MS medium plus 3% sucrose, 0.1 mg l−1 NAA, 0.5 mg l−1 kinetin and 0.0–1.0 mg l−1 ancymidol) and grown for eight weeks. Ancymidol enhanced embryo maturation and germination and was more critical in the solid than liquid medium. Total embryo number did not vary among most treatments. The best response was observed when ancymidol concentrations were 0.1 and 0.5 mg l−1 in the liquid and solid media, respectively; two-thirds of the embryos produced were bipolar and 35% of bipolar embryos germinated. Seven to 82% of plants recovered from different ancymidol treatments were haploid; the others were diploid, triploid or chimeric for ploidy level.

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naphthaleneacetic acid


2,4-dichlorophenoxyacetic acid

GA3 :

gibberellic acid


Murashige and Skoog (1962)


  1. Adler PR, Dufault RJ, Water L (1985) HortScience 20:196–198

  2. Chin CK (1982) HortScience 17:590–591

  3. Chin CK, Khunachak A (1984) Pl Physiol Suppl 75:100

  4. Desjardins Y, Tiessen H, Harney PM (1987) HortScience 22:131–133

  5. Feng XR, Wolyn DJ (1991) Pl Cell Rep 10:574–578

  6. Grant WF (1982) In: Klekorski EJ (ed) Environmental Mutagenesis, Carcinogenesis and Plant Biology Vol II, Praeger, New York, pp 10–11

  7. Hsu JY, Yen CC, Yang WC, Lin WC, Tsay HS (1990) Acta Hort 271:135–143

  8. Levi A, Sink KC (1990) J Pl Physiol 137:184–189

  9. Levi A, Sink KC (1991) Pl Cell Rep 10:71–75

  10. Levi A, Sink KC (1992) Pl Cell Tissue Organ Culture 31:115–122

  11. Murashige T, Skoog F (1962) Physiol Plant 15:473–497

  12. Reuther G, (1977) Acta Hort 78:217–224

  13. Saito T, Nishizawa S, Nishimura S (1991) Pl Cell Rep 10:230–234

  14. Steward FC, Mapes MO (1971) Bot Gaz 133:70–79

  15. Wilmar C, Hellendoorn M (1968) Nature 217:369–370

  16. Zhan XC (1983) Acta Phytophysiol Sinica 9:317–325

  17. Zhu Z (1982) Plant Chromosome Techniques, Academic Press, Beijing, China

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Communicated by E. D. Earle

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Feng, X.R., Wolyn, D.J. Development of haploid asparagus embryos from liquid cultures of anther-derived calli is enhanced by ancymidol. Plant Cell Reports 12, 281–285 (1993).

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  • Sucrose
  • Glutamine
  • Liquid Culture
  • Kinetin
  • Ploidy Level