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Substance P-related peptides in the hypothalamus of Amphibia

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The occurrence of Substance P-(SP)-related peptides in the hypothalamus of three species of Amphibia (newt, clawed, toad, frog) was studied immunohistochemically employing the indirect immunofluorescence method or a double-step technique (indirect immunofluorescence followed by the peroxidase-antiperoxidase complex method). SP-like immunopositive fibers are seen throughout the hypothalamus. They are especially abundant in the preoptic area and in the outer zone of the median eminence, suggesting a role of SP-related peptides in the hypothalamo-hypophysial regulation in these animals. Some SP-like neurons are seen in the posterior hypothalamus and in the preoptic area. In the newt, such SP-like immunopositive neurons occur frequently in the preoptic periventricular grey.

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Correspondence to Dr. Giovanni Gaudino.

Additional information

Work performed under the C.N.R. project “Biologia della Riproduzione”

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Gaudino, G., Fasolo, A. Substance P-related peptides in the hypothalamus of Amphibia. Cell Tissue Res. 211, 241–250 (1980). https://doi.org/10.1007/BF00236447

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Key words

  • Substance P-related peptides
  • Immunohistochemistry
  • Hypothalamus
  • Amphibia (Triturus cristatus, Xenopus laevis, Rana esculenta)