The occurrence of Substance P-(SP)-related peptides in the hypothalamus of three species of Amphibia (newt, clawed, toad, frog) was studied immunohistochemically employing the indirect immunofluorescence method or a double-step technique (indirect immunofluorescence followed by the peroxidase-antiperoxidase complex method). SP-like immunopositive fibers are seen throughout the hypothalamus. They are especially abundant in the preoptic area and in the outer zone of the median eminence, suggesting a role of SP-related peptides in the hypothalamo-hypophysial regulation in these animals. Some SP-like neurons are seen in the posterior hypothalamus and in the preoptic area. In the newt, such SP-like immunopositive neurons occur frequently in the preoptic periventricular grey.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Alpert LC, Drawer JR, Jackson IMD, Reichlin S (1976) Localization of LH-RH in neurons in frog brain (Rana pipiens and Rana catesbeiana). Endocrinology 98:910–921
Brown M, Vale W (1979) Bombesin- a putative mammalian neurogastrointestinal peptide. Trends in Neurosciences 2:95–97
Brownstein ME, Mroz EA, Kizer JS, Palkovits M, Leeman S (1976) Regional distribution of substance P in the brain of the rat. Brain Res 116:299–305
Chan-Palay V, Jonsson G, Palay SL (1978) Serotonin and substance P coexist in neurons of the rat's central nervous system. Proc Natl Acad Sci (USA) 75:1582–1586
Chihara K, Aritnura A, Coy DH, Schally VA (1978) Studies on the interaction of endorphins, substance P and endogenous somatostatin in growth hormone and prolactin release in rats. Endocrinology 102:281–290
Coons AH (1958) Fluorescent antibody methods. In: Danielli JF (ed) General cytochemical methods. Academic Press, New York
Cuello AC, Jessel T, Kanazawa I, Iversen LL (1977) Substance P: localization in synaptic vesicles in rat central nervous system. J Neurochem 29:747–751
Cuello AC, Kanazawa I (1978) The distribution of substance P immunoreactive fibers in the rat central nervous system. J Comp Neurol 178:129–156
Cuello AC, Galfrè G, Milstein C (1979) Detection of substance P in the central nervous system by a monoclonal antibody. Proc Natl Acad Sci (USA) 76:3532–3536
Dierickx K, Vanderberghe MP, Goossens N (1973) Identification of adenohypophysiotropic neurohormone producing neurosecretory cells in Rana temporaria. Z Zellforsch 142:479–513
Doerr-Schott J, Dubois MP (1976) LH-RH-like system in the brain on Xenopus laevis Daud. Immunohistochemical identification. Cell Tissue Res 172:477–486
Doerr-Schott J, Dubois MP (1978) Immunohistochemical localization of different peptidergic substances in the brain of amphibians and reptiles. In: Gaillard PG, Boer HH (eds) Comparative endocrinology. Elsevier-North Holland Biomed Press, Amsterdam
Doerr-Schott J, Garaud JC, Clauss RO (1979) Immunohistochemical localization of a gastrin-like peptide in the brain of an amphibian, Xenopus laevis Daud. Cell Tissue Res 203:65–78
Erspamer V, Falconieri Erspamer G, Linari G (1977) Occurrence of tachykinins (Physalemin or Substance P-like peptides) in the amphibian skin and their actions on smooth muscle preparations. In: Euler US von, Pernow B (eds). Substance P, Raven Press, New York
Euler US von, Gaddum JH (1931) An unidentified depressor substance in certain tissue extracts. J Physiol (Lond) 72:74–87
Fasolo A, Franzoni MF, Mazzi V (1973) The neurohypophysis of the crested newt. III. Fine structure of the median eminence. Z Zellforsch 141:203–221
Fasolo A, Franzoni MF (1974) Golgi impregnation study on the liquor contacting cells bordering the preoptic recess in amphibians. Arch Ital Anat Embriol LXXIX, 153–158
Fasolo A, Franzoni MF (1976) The neurohypophysis of Urodela: a comparative analysis. Monitore Zool Ital (N.S.) 10:149–189
Fasolo A, Franzoni MF (1977) A Golgi study on the hypothalamus of Amphibia, the neuronal typology. Cell Tissue Res 178:341–354
Goos Th MJ, Ligtenberg PJM, van Oordt PGWJ (1976) Immunofluorescence studies on gonadotropin releasing hormone (GRH) in the fore-brain and the neurohypophysis of the green frog, Rana esculenta L. Cell Tissue Res 168:325–333
Hanke W (1976) Neuroendocrinology. In: Llinas R, Precht W (eds) Frog Neurobiology. Springer Verlag, Berlin Heidelberg New York
Hökfelt T, Liungdahl Å, Terenius L, Elde R, Nilsson G (1977) Immunohistochemical analysis of peptide pathways possibly related to pain and analgesia mechanism: Enkephalin and Substance P. Proc Natl Acad Sci (USA) 74:3081–3085
Hökfelt T, Pernow B, Nilsson G, Wetterberg L, Goldstein M, Jeffcoate SL (1978) Dense plexus of substance P immunoreactive nerve terminals in eminentia medialis of the primate hypothalamus. Proc Natl Acad Sci (USA) 75:1013–1015
Konishi S, Otsuka M (1974) The effects of substance P and other peptides on spinal neurons of the frog. Brain Res 65:397–410
Leeman SE, Mroz EA (1974) Minireview: substance P. Life Sci 15:2033–2041
Moody TW, Bert CB (1979) Bombesin-like peptides in rat brain: quantitation and biochemical characterization. Biochem Biophys Res Commun 90:7–14
Pearse AGE (1976) Peptides in brain and intestine. Nature 262:92–94
Rémy C, Dubois MP (1978) Immunofluorescence of somatostatin-producing sites in the hypothalamus of the tadpoles of Alytes obstetricans Laur. Cell Tissue Res 187:315–322
Reubi JC, Jessel TM (1978) Distribution of substance P in the pigeon brain. J Neurochem 31:359–362
Rodriguez EM (1972) Comparative and functional morphology of the median eminence. In: Knigge KM, Weindl A (eds) Brain-endocrine interaction. Median eminence: structure and function. Karger, Basel
Sternberger L (1974) Immunocytochemistry. Prentice Hall, Englewood Cliffs, N.J.
Takahashi T, Otsuka M (1975) Regional distribution of substance P in the spinal cord and nerve roots of the cat and the effect of dorsal root section. Brain Res 87:1–11
Vandesande F (1979) A critical review of immunocytochemical methods for light microscopy. J Neurosci Methods 1:3–23
Vigh B, Vigh-Teichmann L (1973) Comparative ultrastructure of the cerebrospinal fluid containing neurons. Int Rev Cytol 35:189–251
Work performed under the C.N.R. project “Biologia della Riproduzione”
About this article
Cite this article
Gaudino, G., Fasolo, A. Substance P-related peptides in the hypothalamus of Amphibia. Cell Tissue Res. 211, 241–250 (1980). https://doi.org/10.1007/BF00236447
- Substance P-related peptides
- Amphibia (Triturus cristatus, Xenopus laevis, Rana esculenta)