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Somatic embryogenesis and plant regeneration from callus cultures of chickpea (Cicer arietinum L.)

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Abstract

Somatic embryogenesis and plant regeneration were obtained from immature leaflet callus of chickpea. Numerous globular embryos developed on the surface of callus on Murashige and Skoog's (1962) medium containing 25 μM 2,4-dichlorophenoxyacetic acid. These globular embryos differentiated into mature somatic embryos upon removal of 2,4-dichlorophenoxyacetic acid. The maturation of embryos was significantly affected by pH, photoperiod, abscisic acid and genotype. Callus continued to produce somatic embryos for over 8 subcultures at 4 week intervals. Two per cent of the embryos formed plants on medium containing 15 μM gibberellic acid and 1 μM indole-3-butyric acid. Desiccation of embryos for a period of 3 d increased their rate of conversion into plants from 0.9 to 2.8%. All regenerated plants showed normal morphological characteristics.

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Abbreviations

2,4-D:

2,4-dichlorophenoxyacetic acid

NAA:

1-naphthaleneacetic acid

Kn:

Kinetin

BA:

6-benzyladenine

TDZ:

Thidiazuron

GA3 :

Gibberellic acid

IBA:

Indole-3-butyric acid

ABA:

Abscisic acid

AC:

Activated charcoal

AgNO3 :

Silver nitrate

PVP:

Polyvinylpyrrolidone-40,000 mol wt

MS:

Murashige and Skoog (1962) medium

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Communicated by G. C. Phillips

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Barna, K.S., Wakhlu, A.K. Somatic embryogenesis and plant regeneration from callus cultures of chickpea (Cicer arietinum L.). Plant Cell Reports 12, 521–524 (1993). https://doi.org/10.1007/BF00236100

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Keywords

  • Morphological Characteristic
  • Somatic Embryo
  • Somatic Embryogenesis
  • Plant Regeneration
  • Abscisic Acid