Microspore culture was successfully carried out with four cultivars of swede (Brassica napus ssp. rapifera). Embryo yields increased markedly if the culture medium was replaced by fresh medium after a culture period of three days, with more than 200% increase with the highly responding cultivar Gry and more than 800% with the poorly responding cultivar Stenhaug. If the medium was changed at a later time, embryo production declined sharply. Transfer of microspores after 3 days of culture to a medium in which microspores of a different cultivar had been cultured for 3 days had no effect on embryogenesis. Bud size had a large effect on embryo production, particularly with the least responsive cultivar, Stenhaug.
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Communicated by K. Glimelius
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Hansen, M., Svinnset, K. Microspore culture of swede (Brassica napus ssp. rapifera) and the effects of fresh and conditioned media. Plant Cell Reports 12, 496–500 (1993). https://doi.org/10.1007/BF00236094
- Brassica napus ssp. rapifera
- microspore culture
- fresh medium
- conditioned medium