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Relationship between amblyopia, LGN cell ‘shrinkage’ and cortical ocular dominance in cats

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Summary

Six cats were reared with surgically produced squint or atropinisation of one eye during the sensitive period of development. Five cats were reared without any ocular interference but in the same environment as the experimental cats. Four of these normally reared cats provided control data for perikaryal size.

When the cats were 5–8 months old, the ocular dominance distribution of cells in area 17 of the visual cortex was determined, and measurements of visual acuity of cells in the LGN receiving inputs from the area centralis were carried out. Following the neurophysiological experiments, the perikaryal sizes of LGN cells receiving fibres from the area centralis of the left and right eye were measured from Nissl stained sections of the brain of each cat.

Cats which showed greater amblyopia (loss of acuity) of LGN cells driven from the area centralis of the experimental eye, showed a greater degree of apparent ‘shrinkage’ of Nissl stained LGN cells and a greater proportion of cortical cells excited by the control eye than by the experimental eye.

All experimental cats showed a loss of binocularly driven cells, regardless of whether their LGN cells were amblyopic or not, and whether they had ‘shrunk’ or not. However, when LGN cell amblyopia was present, the degree of amblyopia and ‘shrinkage’ of the LGN cells were correlated with the degree of loss of binocular cells also.

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References

  1. Baker FH, Grigg P, von Noorden GK (1974) Effects of visual deprivation and strabismus on the response of neurones in the visual cortex of the monkey, including studies on the striate and prestriate cortex in the normal animal. Brain Res 66: 185–208

  2. Blakemore CB, Eggers H (1978) Animal models for human visual development. In: Cool SJ, Smith EL (eds) Frontiers in visual science. Springer, New York, pp 651–659

  3. Cragg B, Anker R, Wan YK (1976) The effect of age on the reversibility of cellular atrophy in the LGN of the cat following monocular deprivation. A test of two hypotheses about cell growth. J Comp Neurol 168: 345–354

  4. Crawford MLJ, von Noorden GK (1980) Optically induced comitant strabismus in monkeys. Invest Ophthalmol Vis Sci 19: 1105–1109

  5. Derrington AM, Hawken MJ (1980) Effects of visual deprivation on cat LGN neurones. J Physiol (Lond) 300: 61–62P

  6. Dürsteler MR, Garey LJ, Movshon JA (1976) Reserval of the morphological effect of monocular deprivation in the kitten's lateral geniculate nucleus. J Physiol (Lond) 261: 189–210

  7. Garey LJ, Blakemore C (1977) The effects of monocular deprivation on different neuronal classes in the lateral geniculate nucleus of the cat. Exp Brain Res 28: 259–278

  8. Garey LJ, Vital-Durand F (1978) Reversal of morphological effects of monocular deprivation in monkeys. J Physiol (Lond) 276: 49–50P

  9. Guillery RW (1972) Binocular competition in the control of geniculate cell growth. J Comp Neurol 144: 117–130

  10. Guillery RW, Stelzner DJ (1970) The differential effects of unilateral lid closure upon the monocular and binocular segments of the dorsal lateral geniculate nucleus in the cat. J Comp Neurol 139: 413–422

  11. Headon MP, Powell TPS (1973) Cellular changes in the lateral geniculate nucleus of infant monkeys after suture of the eyelids. J Anat 116: 135–145

  12. Headon MP, Powell TPS (1978) The effect of bilateral eye closure upon the lateral geniculate nucleus in Infant monkeys. Brain Res 143: 147–154

  13. Hickey TL, Spear PD, Kratz KE (1976) Quantitative studies of cell size in the cat's dorsal lateral geniculate nucleus following visual deprivation. J Comp Neurol 172: 265–282

  14. Hoffmann K-P, Sireteanu R (1977) Interlaminar differences in the effects of early and late monocular deprivation on the visual acuity of cells in the lateral geniculate nucleus of the cat. Neurosci Lett 5: 171–175

  15. Hubel, DH, Wiesel TN (1962) Receptive fields, binocular interaction and functional architecture in the cat's visual cortex. J Physiol (Lond) 160: 106–154

  16. Hubel DH, Wiesel TN (1965) Binocular interaction in striate cortex of kittens reared with artificial squint. J Neurophysiol 28: 1041–1059

  17. Ikeda H, Tremain KE (1977) Different causes for amblyopia and loss of binocularity in squinting kittens. J Physiol (Lond) 269: 26–27P

  18. Ikeda H, Tremain KE (1978a) The development of spatial resolving power of LGN cells and its susceptibility to blur and strabismus. Arch Ital Biol 116: 375–384

  19. Ikeda H, Tremain KE (1978b) Amblyopia resulting from penalisation: neurophysiological studies of kittens reared with atropinisation of one or both eyes. Br J Ophthalmol 62: 21–28

  20. Ikeda H, Tremain KE (1979a) The ‘shrinkage’ of LGN cells, amblyopia and cortical ocular dominance. J Physiol (Lond) 293: 57P

  21. Ikeda H, Tremain KE (1979b) Amblyopia occurs in retinal ganglion cells in cats reared with convergent squint without alternating fixation. Exp Brain Res 35: 559–582

  22. Ikeda H, Plant GT, Tremain KE (1977) Nasal field loss in kittens reared with convergent squint. Neurophysiological and morphological studies of the lateral geniculate nucleus. J Physiol (Lond) 270: 345–366

  23. Ikeda H, Tremain KE, Einon G (1978) Loss of spatial resolution of lateral geniculate nucleus neurones in kittens raised with convergent squint produced at different stages in development. Exp Brain Res 31: 207–220

  24. Ikeda H, Wright MJ (1972) Simple behavioural tests for defective vision in cats (Film). J Physiol (Lond) 226: 1–2P

  25. Ikeda H, Wright MJ (1976) Properties of LGN cells in kittens reared with convergent squint. A neurophysiological demonstration of amblyopia. Exp Brain Res 25: 63–77

  26. Kupfer C, Palmer P (1964) Lateral geniculate nucleus. Histological and cytochemical changes following afferent denervation and visual deprivation. Exp Neurol 9: 400–409

  27. Lehmkühle S, Kratz K, Mangel SC, Sherman SM (1980) Effects of early monocular lid suture on spatial and temporal sensitivity of neurones in dorsal lateral geniculate nucleus of the cat. J Neurophysiol 43: 542–556

  28. Maffei L, Fiorentini A (1974) Change of binocular properties of the simple cells of the cortex in adult cats following immobilisation of one eye. Vision Res 14: 217–218

  29. Maffei L, Fiorentini A (1976) Monocular deprivation in kittens impairs the spatial resolution of geniculate neurones. Nature 264: 754–755

  30. Matthews MR, Cowan WM, Powell IPS (1960) Transneuronal cells degeneration in the lateral geniculate nucleus of the macaque monkey. J Anat 94: 145–169

  31. Mitzdorf U, Neumann G (1980) Effects of monocular deprivation in the lateral geniculate nucleus of the cat. A analysis of evoked potentials. J Physiol (Lond) 304: 221–230

  32. Mitzdorf U, Singer W (1978) Basic patterns of synaptic activity in visual cortex of normal and monocularly deprived cats. A current source density analysis of electrically evoked potentials. J Physiol (Lond) 284: 120P

  33. Mitzdorf U, Singer W (1980) Monocular activation of visual cortex in normal and monocularly deprived cats. An analysis of evoked potentials. J Physiol (Lond) 304: 203–220

  34. Movshon JA, Dürsteler MR (1977) Effects of brief periods of unilateral eye. closure on the kittens's visual system. J Neurophysiol 40: 1255–1265

  35. Noorden GK cvon (1973) Histological studies of the visual system in monkeys with experimental amblyopia. Invest Ophthalmol 12: 727–738

  36. Noorden GK von, Middleditch PR (1975) Histology of the monkey lateral geniculate nucleus after unilateral lid closure and experimental strabismus. Further observations. Invest Ophthalmol 14: 674–683

  37. Sanderson KJ (1971) The projecton of the visual field to the lateral geniculate and medial interlaminar nuclei in the cat. J Comp Neurol 143: 101–118

  38. Shapley R, Tat So Y (1980) Is there an effect of monocular deprivation on the proportions of X and Y cells in the cat lateral geniculate nucleus. Exp Brain Res 39: 41–48

  39. Sherman SM (1979) The functional significance of X and Y cells in normal and visually deprived cats. Trends Neurosci 2: 192–195

  40. Sherman SM, Guillery RW, Kaas JH, Sanderson KJ (1974) Behavioural, electrophysiological and morphological studies of binocular competition in the development of the geniculocortical pathways of cats. J Comp Neurol 158: 1–18

  41. Sherman SM, Hoffmann K-P, Stone J (1972) Loss of a specific cell type from dorsal lateral geniculate nucleus in visually deprived cats. J Neurophysiol 35: 532–541

  42. Sherman SM, Wilson JR, Guillery RW (1975) Evidence that binocular competition affects the postnatal development of Y cells in the cat's lateral geniculate nucleus. Brain Res 100: 441–444

  43. Singer W (1977) Effects of monocular deprivation on excitatory and inhibitory pathways in cat striate cortex. Exp Brain Res 30: 25–41

  44. Singer W, Yinon U, Tretter F (1979) Inverted monocular vision prevents ocular dominance shifts in kittens and impairs the functional state of visual cortex in adult cats. Brain Res 164: 294–299

  45. Vital-Durand F, Garey LJ, Blakemore C (1978) Monocular and binocular deprivation in the monkey. Morphological effects and reversibility. Brain Res 158: 45–64

  46. Wiesel TN, Hubel DH (1963) Effects of visual deprivation on morphology and physiology of cells in the cat's lateral geniculate body. J Neurophysiol 26: 979–993

  47. Yinon UL (1976) Age dependence of the effect of squint on cells in the kitten's visual cortex. Exp Brain Res 26: 151–157

  48. Yinon UL, Auerbach E, Blank M, Friesenhausen J (1975) The ocular dominance of cortical neurones in cats developed with divergent and convergent squint. Vision Res 15: 1251–1256

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Correspondence to H. Ikeda DSc.

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Supported by grants from the MRC and St. Thomas' Hospital

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Tremain, K.E., Ikeda, H. Relationship between amblyopia, LGN cell ‘shrinkage’ and cortical ocular dominance in cats. Exp Brain Res 45, 243–252 (1982). https://doi.org/10.1007/BF00235784

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Key words

  • Amblyopia
  • Strabismus
  • Penalisation
  • Ocular dominance
  • Perikaryal size