Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Comparison of terpenoid indole alkaloid production and degradation in two cell lines of Tabernaemontana divaricata

  • 34 Accesses

  • 14 Citations

Abstract

Two cell lines of Tabernaemontana divaricata derived from the same suspension culture accumulate different amounts of the terpenoid indole alkaloids O-acetylvallesamine and voaphylline. [15N]O-acetylvallesamine and [15N]voaphylline were added to the suspension cultures to investigate whether the lack of accumulating capacity of one of the cell lines was due to a low biosynthetic ability or to high turnover rates. The difference was shown to be due to the inability of the cell culture to biosynthesize both alkaloids. Both cell lines were able to metabolize O-acetylvallesamine. This metabolisation occurred mainly during the stationary phase. The alkaloids added were chemically unstable under culture conditions. Under normal batch cell culture conditions chemical breakdown is thought to play a minor role in the total amount of compound transformed.

This is a preview of subscription content, log in to check access.

Abbreviations

GC-MS:

gas chromatography-mass spectrometry

HPLC-UV:

high performance liquid chromatography-ultra violet

rpm:

rotations per minute

References

  1. Dagnino D, Schripsema J, Peltenburg-Looman A, Verpoorte R, Teunis K (1991) J Nat Prod 54:1558–1563

  2. Dagnino D, Schripsema J, Verpoorte R (1993) Phytochemistry 32:325–329

  3. dos Santos RI, Schripsema J, Verpoorte R Phytochemistry, in press (1993)

  4. Ghini AA, Burton G, Gros EG (1991) Phytochemistry 30:779–784

  5. Goodbody A, Endo T, Vukovic J, Misawa M (1988) Planta Medica 210–214

  6. Luckner M (1990) Secondary Metabolism in Microorganisms Plants and Animals. 3rd edn, Springer, Berlin Heidelberg New York

  7. Murashige T, Skoog F (1962) Physiol Plant 15:473–497

  8. Sariaslani FS, Duffel MW Rosazza JP (1985) J Med Chem 28:629–633

  9. Schripsema J, Meijer AH, Iren F van, Hoopen HJG ten, Verpoorte R (1990) Plant Cell Tissue Org Cult 8:153–161

  10. Schripsema J, Verpoorte R (1992) Planta Med 58:245–249

  11. Sierra MI (1991) Ph D Thesis, University of Leiden, The Netherlands, pp 67–75

  12. Sundberg RJ (1970) The Chemistry of Indoles. Academic Press, London

Download references

Author information

Additional information

Communicated by W. Barz

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Dagnino, D., Schripsema, J. & Verpoorte, R. Comparison of terpenoid indole alkaloid production and degradation in two cell lines of Tabernaemontana divaricata . Plant Cell Reports 13, 95–98 (1993). https://doi.org/10.1007/BF00235298

Download citation

Keywords

  • Alkaloid
  • Suspension Culture
  • Terpenoid
  • Tryptamine
  • Indole Alkaloid