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Agrobacterium-mediated transformation of Citrus stem segments and regeneration of transgenic plants

Summary

A method for Agrobacterium-mediated transformation of Citrus and organogenic regeneration of transgenic plants is reported. Internodal stem segments were co-cultured with Agrobacterium harboring binary vectors that contained the genes for the scorable marker ß-glucuronidase (GUS) and the selectable marker NPT-II. A low but significant percentage (≤ 5%) of the shoots regenerated in the presence of 100 μg/ml kanamycin were GUS+. Polymerase chain reaction (PCR) analysis confirmed that GUS+ shoots contained T-DNA. Two plants established in soil were shown to be transgenic by Southern analysis.

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Communicated by G. C. Phillips

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Moore, G.A., Jacono, C.C., Neidigh, J.L. et al. Agrobacterium-mediated transformation of Citrus stem segments and regeneration of transgenic plants. Plant Cell Reports 11, 238–242 (1992). https://doi.org/10.1007/BF00235073

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Key words

  • Carrizo citrange
  • Key lime (C. aurantifolia)
  • Genetic transformation