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Role of the pia mater in the transfer of substances in and out of the cerebrospinal fluid

Summary

The transfer of 14C leucine, 14C GABA and 14C urea from an isolated CSF compartment has been studied by implanting plastic cups on the cerebral cortex of anaesthetized cats under different experimental conditions. Removal of the pia mater resulted in a significant decrease in the disappearance of the substances from the cups. The intravenous infusion of GABA also stopped the escape of this amino acid from the plastic cups. When the cups contained only artificial CSF, and GABA was infused intravenously, its appearance in the cups followed a diffusional pattern. Ligaturing the sagittal venous sinus decreased the rate of disappearance of 14C urea from the plastic cups. Higher concentrations of GABA and leucine were found in the pial membrane than in the underlying brain tissue, after these amino acids in solution were left in the cortical cups for 5 hours. Pressure applied during implantation of the cups sometimes caused accumulation of fluid in the cups.

It is suggested that the pial membrane and its vasculature play an important role in the transfer of substances in and out of the subarachnoid space.

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References

  1. Altman, J., Chorover, S.L.: Autoradiographic investigation of the distribution and utilisation of intraventricularly injected adenine−3H, uracil−3H and thymidine−3H in the brain of cats. J. Physiol. (Lond.) 169, 770–779 (1963).

  2. Bering, E.A., Sato, O.: Hydrocephalus: changes in formation and absorption of cerebrospinal fluid within the cerebral ventricles. J. Neurosurg. 20, 1050–1063 (1963).

  3. Bertler, A., Falck, B., Owman, Ch., Rosengreen, E.: The localization of monoaminergic blood-brain barrier mechanisms. Pharmacol. Rev. 18, 369–385 (1966).

  4. Bito, L.Z., Bradbury, M.W.B., Davson, H.: Factors affecting the distribution of iodide and bromide in the central nervous system. J. Physiol. (Lond.) 185, 323–354 (1966).

  5. Celesia, G.G., Jasper, H.H.: Acetylcholine released from cerebral cortex in relation to state of activation. Neurology (Minneap.) 16, 1053–1064 (1966).

  6. Crowshaw, K., Jessup, S.J., Ramwell, P.W.: Thin layer chromatography of l-dimethylamino-naphtalene-5-sulphonyl derivatives of amino acids present in superfusates of cat cerebral cortex. Biochem. J. 103, 79–85 (1967).

  7. Davson, H., Kleeman, C.R., Levin, E.: Quantitative studies of the passage of different substances out of the cerebrospinal fluid. J. Physiol. (Lond.) 161, 126–142 (1962).

  8. Eccleston, D., Randič, M., Roberts, M.H.T., Straughan, D.W.: Release of amines and amine metabolites from brain by neural stimulation. In: “Metabolism of Amines in the Brain”, pp. 29–33. Ed. by G. Hooper. London: Macmillan 1968.

  9. Elliott, K.A.C., Jasper, H.H.: Physiological salt solutions for brain surgery. Studies of local pH and pial vessel reactions to buffered and unbuffered isotonic solutions. J. Neurosurg. 6, 140–152 (1949).

  10. — Swank, R.L., Henderson, N.: Effects of anesthetics and convulsants on acetylcholine content of brain. Amer. J. Physiol. 162, 469–474 (1950).

  11. — Van Gelder, N.M.: Occlusion and metabolism of γ-aminobutyric acid by brain tissue. J. Neurochem. 3, 28–40 (1958).

  12. Fernstermacher, J.D., Li, C., Levin, V.A.: Extracellular space of the cerebral cortex of normothermic and hypothermic cats. Exp. Neurol. 27, 101–114 (1970).

  13. Hochwald, G.M., Wallenstein, M.: Exchange of albumin between blood, cerebrospinal fluid and brain in the cat. Amer. J. Physiol. 212, 1199–1204 (1967).

  14. Jasper, H.H., Khan, R.T., Elliott, K.A.C.: Amino acids released from the cerebral cortex in relation to its state of activation. Science 147, 1448–1449 (1965).

  15. Kaplan, H.A., Ford, D.H.: The brain vascular system, p. 152. Amsterdam: Elsevier 1966.

  16. Levin, E., Cristobal, C., Scicli, G.: Incubation of brain tissue ‘in vivo’. Amino acid uptake. Arch. int. Physiol. 79, 117–126 (1971).

  17. — Duvilanski, B.: Incorporation of glutamic acid into the cat caudate nucleus. Acta physiol. lat.-amer. 19, 351–357 (1969).

  18. — Garcia Argiz, C.A., Nogueira, G.J.: Ventriculo-cisternal perfusion of amino acids in cat brain. II. Incorporation of glutamic acid, glutamine and GABA into the brain parenchyma. J. Neurochem. 13, 979–988 (1966).

  19. — Kleeman, C.R.: Significance of different routes of introduction on the transport of substances into the brain. Proc. Soc. exp. Biol. (N.Y.) 135, 685–689 (1970).

  20. — Scicli, G.: Brain barrier phenomena. Brain Res. 13, 1–12 (1969).

  21. — Wolosiuk, R., Scicli, G., Glanczpigel, R.: In vitro incubation of brain hemispheres. Fluid spaces and amino acid uptake. Neurology (Minneap.) 20, 584–593 (1970).

  22. MacIntosh, F.C., Oborin, P.E.: Release of acetylcholine from intact cerebral cortex. Abstr. XIX Intern. Physiol. Congr. 1953, Montreal, 1953: 580–581.

  23. Maiorova, N.A.: Citofisiologicheskoe issledovanie arachnoidendotelia miagkoi obolochki mozaga v sviazi s zaschitnoi funkciei sistemi iachei. Vop. Neirokhir. 25, 8–14 (1961).

  24. Maiorova, N.A. Rol zaschitno-troficheskoi sistemi iachei miagkoi obolochki mozga v udalenii aminokislot i belka is likvora. Bull. exp. Biol. Med. 54, 107–110 (1962).

  25. Merlis, J.K.: The effect of changes in the calcium content of the cerebrospinal fluid on spinal reflex activity in the dog. Amer. J. Physiol. 131, 67–72 (1940).

  26. Milhorat, T.H.: Choroid plexus and cerebrospinal fluid production. Science 166, 1514–1516 (1969).

  27. Nogueira, G.J., Garcia Argiz, C.A., Levin, E.: Disappearance of different substances in contact with the external surface of the brain. Experientia (Basel) 21, 734–736 (1965).

  28. Pappius, H.M., McCann, W.P.: Effects of steroids on cerebral edema in cats. Arch. Neurol. (Chic.) 20, 207–216 (1969).

  29. Pollay, M., Curl, F.: Secretion of cerebrospinal fluid by the ventricular ependyma of the rabbit. Amer. J. Physiol. 213, 1031–1038 (1967).

  30. — Kaplan, R.J.: Diffusion of non-electrolytes in brain tissue. Brain Res. 17, 407–416 (1970).

  31. Ramwell, P.W., Shaw, J.E.: Spontaneous and evoked release of prostaglandins from the cerebral cortex of anaesthetized cats. Amer. J. Physiol. 211, 125–134 (1966).

  32. Roberts, E., Kuriyama, K.: Biochemical — physiological correlations in studies of the γ-aminobutyric acid system. Brain Res. 8, 1–35 (1968).

  33. Schaltenbrand, G., Putnam, T.: Untersuchungen zum Kreislauf der liquor cerebrospinalis mit Hilfe intravenöser Fluorescineinspritzungen. Dtsch. Z. Nervenheilk. 96, 123–132 (1927).

  34. Van Harreveld, A., Ahmed, N.: Release of intravenously administered iodide from the pial and dural surfaces. Brain Res. 11, 32–41 (1968).

  35. — Kooiman, M.: Amino acid release from the cerebral cortex during spreading depression and asphyxiation. J. Neurochem. 12, 431–439 (1965).

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Weight, P.M., Nogueira, G.J. & Levin, E. Role of the pia mater in the transfer of substances in and out of the cerebrospinal fluid. Exp Brain Res 13, 294–305 (1971). https://doi.org/10.1007/BF00234951

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Key Words

  • Pia mater
  • Blood brain barrier
  • Pial vasculature
  • CSF formation
  • Meningeal permeability