Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Spontaneous somatic embryogenesis and plant regeneration from root cultures of Peucedanum palustre

Abstract

The regeneration of Peucedanum palustre (L.) Moench (milk parsley) was established for the first time via somatic embryogenesis from primary root cultures. Callus formation occurred on the root cultures and showed spontaneous embryogenic capability on B5 basal medium supplemented with a low concentration of indoleacetic acid (5.5 × 10−7 M). 2,4-Dichlorophenoxyacetic acid was not needed for the initiation of embryogenesis. The somatic embryos germinated and formed plantlets on hormone-free B5 medium. These plantlets were easily transferable to pots, and are presently passing their second growing season in the greenhouse.

Development of the somatic embryos progressed through the globular, heart-shaped, torpedo-shaped, and cotyledonary stages, typical of zygotic embryos. Synchronization performed by sieving the embryos did not affect the development time. The culture has retained its embryogenic capacity for 25 months.

This is a preview of subscription content, log in to check access.

Abbreviations

2,4-D:

2,4-Dichlorophenoxyacetic acid

IAA:

indoleacetic acid

IBA:

3-indolebutyric acid

BAP:

6-benzylaminopurine

References

  1. Ammirato PV (1983) In: Evans D (ed) Handbook of plant cell culture. Vol. 1. Techniques for propagation and breeding. MacMillan Publ. Co., New York, pp. 82–123

  2. Borkird C, Choi JH, Sung ZR (1986) Plant Physiol 81:1143–1146

  3. Collin HA, Isaac S (1991) In: Bajaj YPS (eded) Bio-technology in agriculture and forestry 15. Medicinal and aromatic plants III. Springer-Verlag, pp. 73–92

  4. Dudits D, Bögre L, Györgyey J (1991) J Cell Sci 99:473–483

  5. Furmanowa, M., Sowinska, D. and Pietrosiuk, A. (1991) In: Bajaj YPS (ed) Biotechnology in agriculture and forestry 15. Medicinal and aromatic plants III. Springer-Verlag, pp. 176–190

  6. Gamborg O, Miller RA, Qjima K (1968) Exp Cell Res 50:151–158

  7. Horsch RB, King J (1990) Can J Bot 58:2402–2406

  8. Jia J, Shi J, Wang Y, Zhang S (1989) Acta Bot Sin 31:361–366

  9. Mizuta S, Okuda K, Inagawa K, Tanaka I (1987) Mem Fac Sci Kochi Univ Set D (Biol) 8:29–38

  10. Nadel BL, Altman A, Ziv M. (1990) Plant Cell Tiss Org Cult 20:119–124

  11. Oksman-Caldentey K-M, Kivelä O, Hiltunen R (1991) Plant Sci 78:129–136

  12. Pierik RLM (1987) In vitro culture of higher plants. Martinus Nijhoff Publishers, Dordrecht, pp. 139–146, 213–229

  13. Smith SM, Street HE (1974) Ann Bot 38:223–241

  14. Vasil IK (1984) Cell culture and somatic cell genetics of plants I. Academic Press, Inc., Orlando, Florida, 825 p

  15. Vuorela H (1988) Acta Pharm Fenn 97:113–120

  16. Vuorela H, Törnquist K, Sticher O, Hiltunen R. (1988) Acta Pharm Fenn 97:167–174

Download references

Author information

Additional information

Communicated by H. Lörz

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Vuorela, P., Oksman-Caldentey, K., Lipponen, J. et al. Spontaneous somatic embryogenesis and plant regeneration from root cultures of Peucedanum palustre . Plant Cell Reports 12, 453–456 (1993). https://doi.org/10.1007/BF00234711

Download citation

Keywords

  • Germinate
  • Development Time
  • Somatic Embryo
  • Basal Medium
  • Somatic Embryogenesis