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Autoradiographic assessment of 3H-proline uptake by osteoblasts following guanethidine-induced sympathectomy in the rat

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Sympathectomy was carried out in rats by injections of guanethidine-sulfate from birth to 14 days of age. At 45 days of age, the activity of osteoblastic cells was monitored by 3H-proline autoradiography. Effectiveness of sympathectomy was verified by light-microscopic examination of superior cervical and celiac ganglia. Grain counts over periosteal osteoblasts of the femoral diaphysis and osteoblasts mesial to the first molar in the mandible demonstrated a significantly reduced uptake of 3H-proline in the sympathectomized rats. The data provide direct evidence of sympathetic influence on osteoblastic activity and suggest that sympathectomy may result in the loss of a trophic influence which is important in the regulation of osteogenesis.

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Correspondence to Dr. I. J. Singh.

Additional information

Supported by N.I.D.R. grant DEO 4557 (R.M.K.), N.I.H. grant 5-SO1RR-5373 to K.U.M.C., and N.I.H. grant RR-05332 to N.Y.U. (U.S.). We thank Charles A. Brownley of CIBA-Geigy, Summit, N.J. for the guanethidine sulfate

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Singh, I.J., Klein, R.M. & Herskovits, M. Autoradiographic assessment of 3H-proline uptake by osteoblasts following guanethidine-induced sympathectomy in the rat. Cell Tissue Res. 216, 215–220 (1981).

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Key words

  • Autoradiography
  • 3H-proline
  • Osteoblasts
  • Sympathectomy
  • Guanethidine-sulfate