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Autoradiographic assessment of 3H-proline uptake by osteoblasts following guanethidine-induced sympathectomy in the rat

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Summary

Sympathectomy was carried out in rats by injections of guanethidine-sulfate from birth to 14 days of age. At 45 days of age, the activity of osteoblastic cells was monitored by 3H-proline autoradiography. Effectiveness of sympathectomy was verified by light-microscopic examination of superior cervical and celiac ganglia. Grain counts over periosteal osteoblasts of the femoral diaphysis and osteoblasts mesial to the first molar in the mandible demonstrated a significantly reduced uptake of 3H-proline in the sympathectomized rats. The data provide direct evidence of sympathetic influence on osteoblastic activity and suggest that sympathectomy may result in the loss of a trophic influence which is important in the regulation of osteogenesis.

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References

  1. Ascenzi A, Bell GH (1972) Bone as a mechanical engineering problem. In: Bourne GJ (ed) The Biochemistry and Physiology of Bone. Academic Press, New York, p 341–345

  2. Avery JK, Cox CF, Chiego DJ (1980) Presence and location of adrenergic nerve endings in the dental pulps of mouse molars. Anat Rec 198:59–71

  3. Avery JD, Strachan DS, Corpron RE, Cox CF (1971) Morphological studies of the altered pulps of the New Zealand white rabbit after resection of the inferior alveolar nerve and/or the superior cervical ganglion. Anat Rec 171:495–508

  4. Avery JD, Cox CF, Corpron RE (1974) The effects of combined nerve resection and cavity preparation and restoration on response dentine formation in rabbit incisors. Arch Oral Biol 10:539–548

  5. Bacq ZM (1936) The action of abdominal sympathectomy on the growth of the albino rat and the weight of the genital organs. Am J Physiol 95:601–604

  6. Barr JS, Stinchfielf AJ, Reidy JA (1950) Sympathetic ganglionectomy and limb length in poliomyelitis. J Bone Joint Surg 32A: 793–802

  7. Bisgard JD (1938) Longitudinal bone growth. The influence of sympathetic denervation. Ann Surg 97:374–380

  8. Cannon WB, Newton HF, Bright EM, Menkin V, Moore RM (1929) Some aspects of the physiology of animals surviving complete exclusion of sympathetic nerve impulses. Am J Physiol 89:84–107

  9. Chiego DJ, Singh IJ (1981) Evaluation of the effects of sensory denervation on osteoblasts by 3H-proline autoradiography. Cell Tissue Res, in press

  10. Corbin KB, Hinsey JC (1939) Influence of the nervous system on bone and joints. Anat Rec 75:307–317

  11. Drinker CK, Drinker KR (1916) A method for maintaining an artificial circulation through the tibia of the dog, with a demonstration of the vasomotor control of the marrow vessels. Am J Physiol 40:514–521

  12. Dubner R, Sessle BJ, Storey AT (1978) The Neural Basis of Oral and Facial Function. Plenum Press, New York, p 379–390

  13. Duncan CP, Shim SS (1977) The autonomic nerve supply of bone. J Bone Jt Surg 598:323–330

  14. Fell WA (1949) The effect of sympathectomy on the size of Haversian canals of the cat. J Anat 83:67–68

  15. Fontaine PR (1926) A propos de huit cas de retard de consolidation et de pseudarthrose, traités par la sympathectomie périartérielle. Rev Chirurg 64:95–104

  16. Frymoyer JW, Pope MH (1977) Fracture healing in the sciatically denervated rat. J Trauma 17:355–361

  17. Harris RI (1930) The effect of lumbar sympathectomy on the growth of legs shortened from anterior polimyelitis: a preliminary report. J Bone Joint Surg 12:859–866

  18. Harris RI, McDonald JL (1936) The effect of lumbar sympathectomy upon the growth of legs paralyzed by anterior poliomyelitis. J Bone Joint Surg 18:35–45

  19. Hulth A, Semb H (1966) In: Gaillard PJ, Van de Hoff A, Steendijk R (eds) Fourth European symposium on calcified tissures. WC den Ouden, Amsterdam, p 58–59

  20. Johnson EM, Cantor E, Douglas JR (1975) Biochemical and functional evaluation of the sympathectomy produced by the administration of guanethidine to newborn rats. J Pharmacol Exp Ther 193:503–512

  21. Key JA, Moore RM (1933) Healing of fractures, of defect in bone and of defects in cartilage after sympathectomy. Arch Surg 26:272–279

  22. Klein RM (1979) Alteration of neonatal rat parotid gland acinar cell proliferation by guanethidine-induced sympathectomy. Cell Tissue Kinet 12:411–423

  23. Klein RM, Chiego DJ, Avery JK (1981) Effects of chemical sympathectomy on cell proliferation in the progenitive compartment of the neonatal mouse incisor. Arch Oral Biol, in press

  24. Milgram JW, Robinson RA (1965) An electron microscopic demonstration of unmyelinated nerves in the Haversian canals of the adult dog. Bullet Johns Hopkins Hosp 117:163–173

  25. Miller MR, Kasahara M (1963) Observations on the innervation of human long bones. Anat Rec 145:13–23

  26. Pearse HE, Morton JJ (1931) The influence of alterations in the circulation on the repair of bone. J Bone Joint Surg 13:68–74

  27. Reimann I, Christensen SB (1977) A histological demonstration of nerves in subchondral bone. Acta Orthop Scandinav 48:345–352

  28. Sherman MS, McFarland G (1965) Mechanism of pain in osteoid osteomas. South Med J 58:163–166

  29. Taylor AC (1950) Microscopic observation of living tooth pulp. Science 111:40

  30. Taylor AC, Butcher EO (1951) The regulation of eruption rate in the incisor teeth of the white rat. J Exp Zool 117:165–188

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Author information

Correspondence to Dr. I. J. Singh.

Additional information

Supported by N.I.D.R. grant DEO 4557 (R.M.K.), N.I.H. grant 5-SO1RR-5373 to K.U.M.C., and N.I.H. grant RR-05332 to N.Y.U. (U.S.). We thank Charles A. Brownley of CIBA-Geigy, Summit, N.J. for the guanethidine sulfate

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Singh, I.J., Klein, R.M. & Herskovits, M. Autoradiographic assessment of 3H-proline uptake by osteoblasts following guanethidine-induced sympathectomy in the rat. Cell Tissue Res. 216, 215–220 (1981). https://doi.org/10.1007/BF00234556

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Key words

  • Autoradiography
  • 3H-proline
  • Osteoblasts
  • Sympathectomy
  • Guanethidine-sulfate