Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Genotypic and developmental regulation of transient expression of a reporter gene in soybean zygotic cotyledons

Abstract

Processes involved in transformation of regenerable soybean (Glycine max (L.) Merrill) immature zygotic cotyledons were studied by assaying the transient expression of the β-glucuronidase gene driven by the 35S promoter and terminated at the 3′ end by the soybean 7S storage protein gene. The plasmid containing the chimeric gene was delivered to the cotyledons via particle bombardment 900 PSI. Zygotic cotyledons from six soybean varieties were tested for transient expression of the β-glucuronidase gene. The level of reporter gene expression differed between genotypes. The genotypes could be classified as high, fair or poor expressors. Cotyledons from different genotypes were then bombarded at 650, 900 or 1100 PSI. GUS expression varied among genotypes independently of the pressure of bombardment. Finally, the ability of cotyledons to express the reporter gene depended on the developmental stage of the seed from which it was excised with the younger stage being the least responsive. However, genotypic specific expression remained after controlling for developmental stage of the cotyledons.

This is a preview of subscription content, log in to check access.

Abbreviations

ANOVA:

analysis of variance

GUS:

β-glucuronidase

PSI:

pounds per square inch

TEUs:

transient expression units

MSO3:

MS media with 3% sucrose

References

  1. Bailey MA, Boerma HR, Parrott WA (1993) Crop Sci: in press

  2. Christou P, Swain WF, Yang NS, McCabe DE (1989) Proc Nat Acad Sci USA 86: 7500–7504

  3. Christou P, McCabe DE, Martinell BJ, Swain WF (1990) Trends in Biotech 8: 145–151

  4. Finer JF, McMullen D (1991) In Vitro Cell Dev Biol 27: 175–182

  5. Gamborg OL, Miller RA, Ojima K (1968) Exp Cell Res 50: 151–158

  6. Hartweck LM, Lazzerri PA, Cui D, Collins GB, Williams EG (1988) In Vitro Cell Dev Biol 24: 821–828

  7. Hildebrand DF, Liu W, Deng W, Grayburn WS, Collins GB (1991) In: Rattray JBM (ed) Biotechnology and the oils and fats industry. Aberical Oil Chemistry Society Press, Champagne, IL, pp 58–75

  8. Hinchee MA, Conner-Ward DV, Newell CA, McDonnell RE, Sato SJ, Gasser CS, Fischoff DA, Re DB, Fraley RT, Horsch RB (1988) Bio/Tech 6: 915–922

  9. Hood EE, Chilton WS, Chilton M-D, Fraley RT (1986) J Bacteriol 168: 1283–1290

  10. Horsch RB, Fry JE, Hoffman NL, Eichholz D, Rogers SG, Fraley RT (1985) Science 227: 1227–1231

  11. Hooykaas PJJ (1989) Plant Mol Biol 13: 327–336

  12. Jefferson RA (1987) Plant Mol Biol Rep 5: 387–405

  13. Klein TM, Wolf ED, Wu R, Sanford JC (1987) Nature 327: 70–73

  14. Komatsuda T (1992) Bull Natl Inst Agrobiol Resour 7: 1–78

  15. Kudirka DT, Colburn SM, Hinchee MA, Wright MS (1986) Can J Genet Cytol 28: 808–817

  16. Lazzeri PA, Hildebrand DF, Collins GB (1985) Plant Mol Biol Rep 3: 160–167

  17. Li, L, Qu R, Kochko A, Fauquet C, Beachy RN (1993) Plant Cell Rep 12: 250–255

  18. Matthysse AG, Gurlitz HG (1982) Physiol Plant Path 21: 381–387

  19. McCabe DE, Swain WF, Martinell BJ, Cristou P (1988) Bio/Tech 6: 923–926

  20. Moore PJ, Coffins GB (1993) In: Bajaj YPS (ed) Biotechnology in Agriculture and Forestry, vol 23, Springer-Verlag, Berlin Heidelberg New York, pp 228–236

  21. Murashige T, Skoog F (1962) Physiol Plant 15: 473–494

  22. Owens LD, Cress DE (1985) Plant Physiol 77: 87–94

  23. Parrott WA, Dryden G, Vogt S, Hildebrand DF, Collins GB, Williams EG (1988) In Vitro Cell Dev Biol 24: 817–820

  24. Parrott WA, Hoffman LM, Hildebrand DF, Williams EG, Collins GB (1989) Plant Cell Rep 7: 615–617

  25. Parrott WA, Williams EG, Hildebrand DF, Collins GB (1989) Plant Cell, Tiss, Org Cult 16: 15–21

  26. Pederson HC, Cristiansen J, Wyndaele R (1983) Plant Cell Rep 2: 201–204

  27. Sato S, Newell C, Kolacz K, Tredo L, Finer J, Hinchee M (1993) Plant Cell Rep 12: 408–413

  28. Sebastiani F, Farrell LB, Schuler MA, Beachy RN (1990) Plant Mol Biol 15: 197–201

Download references

Author information

Additional information

Communicated by W. A. Parrott

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Moore, P.J., Moore, A.J. & Collins, G.B. Genotypic and developmental regulation of transient expression of a reporter gene in soybean zygotic cotyledons. Plant Cell Reports 13, 556–560 (1994). https://doi.org/10.1007/BF00234510

Download citation

Keywords

  • Reporter Gene
  • Storage Protein
  • Transient Expression
  • Particle Bombardment
  • Reporter Gene Expression