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The effect of temperature on hairy root cultures of Catharanthus roseus: Growth, indole alkaloid accumulation and membrane lipid composition

Abstract

Cultivation of Catharanthus roseus hairy root cultures at different temperatures was found to have an effect on growth rate and indole alkaloid content as well as lipid composition. When lowering the temperature, the roots responded by increasing the degree of unsaturation of cellular lipids, which was mainly due to an increased proportion of linolenic acid in the main lipid classes. The modifications in lipid composition were obviously necessary for the roots to retain the proper cell membrane fluidity at each temperature. Despite of changes in membrane lipids, no effect on the distribution of indole alkaloids between the roots and the medium could be detected. Instead, the level of alkaloid accumulation showed a clear increase with lowering temperature.

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Abbreviations

PC:

phosphatidylcholine

PE:

phosphatidylethanolamine

PI:

phosphatidylinositol

PS:

phosphatidylserine

PG:

phosphatidylglycerol

CL:

cardiolipin

DGD:

digalactosyldiglyceride

MGD:

monogalactosyldiglyceride

NL:

neutral lipids

DU:

degree of fatty acid unsaturation

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Communicated by M. R. Davey

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Toivonen, L., Laakso, S. & Rosenqvist, H. The effect of temperature on hairy root cultures of Catharanthus roseus: Growth, indole alkaloid accumulation and membrane lipid composition. Plant Cell Reports 11, 395–399 (1992). https://doi.org/10.1007/BF00234368

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Keywords

  • Lipid
  • Linolenic Acid
  • Alkaloid
  • Membrane Lipid
  • Lipid Composition