Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

In vitro germination of wheat proembryos to fertile plants

  • 35 Accesses

  • 13 Citations

Summary

Wheat proembryos were excised 7 days after anthesis and germinated to normal fertile plants with an efficiency of 90%. These embryos had clearly differentiated shoot apical meristems and represent an interesting model system for studies of meristem biology. A combination of two media with different osmolarities allowed the continued development of the embryos before induction of germination. Normal germination occurred only after the first leaf primordium had already covered the shoot apical meristem.

This is a preview of subscription content, log in to check access.

References

  1. Dieterich K (1924) Flora 117: 379–417

  2. Haagen-Smit AJ, Siu R, Wilson G (1945) Science 101: 234

  3. Heimann R, Müller M (1991) J Electron Microscopy Tech. 18: 440–449

  4. Iglesias VA, Gisel A, Leduc N, Zhang S, Potrykus I, Sautter C (1993a) J Cellular Biochem 17B: 29

  5. Iglesias VA, Gisel A, Bilang R, Leduc N, Potrykus I, Sautter C (1993b) Planta 192: 84–91

  6. Kent N, Brink RA (1947) Science 106: 547–548

  7. Kent-Ziebur N, Brink RA, Graf LH, Stahlmann MA (1950) Am J Bot 37: 144–148

  8. Kent-Ziebur N, Brink RA (1951) Am J Bot 38: 253–256

  9. King RW (1976) Planta 132: 43–51

  10. Liu C, Xu Z, Chua N-H (1993) Plant J 3: 291–300

  11. Merry J (1941) Bulletin of the Torrey Botanical Club 68: 585–598

  12. Monnier M (1976) Rev Cyt Biol Veg 39: 1–120

  13. Murashige T, Skoog F (1962) Physiol Plant 15: 473–497

  14. Norstog K (1965) Amer J Bot 52: 538–546

  15. Norstog K (1972) Phytomorphology 22: 134–139

  16. Norstog K, Klein R (1972) Can J Bot 50: 1887–1894

  17. Pope MN (1949) J Agric Res 78: 295–309

  18. Radley M (1979) J Exp Bot 30: 381–389

  19. Raghavan V (1986) Embryogenesis in Angiocperms. Cambridge University Press

  20. Rappaport J (1954) Bot Review 20: 201–225

  21. Triplett BA, Quatrano RS (1982) Dev Biol 91: 491–496

Download references

Author information

Additional information

Communicated by P. J. King

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Iglesias, V.A., Gisel, A., Potrykus, I. et al. In vitro germination of wheat proembryos to fertile plants. Plant Cell Reports 13, 377–380 (1994). https://doi.org/10.1007/BF00234141

Download citation

Keywords

  • Germinate
  • Apical Meristem
  • Shoot Apical Meristem
  • Continue Development
  • Fertile Plant