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Plant regeneration from mesophyll protoplasts of Matthiola incana (L.) R. Br.

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Summary

A protocol for obtaining regenerated fertile plants from mesophyll protoplasts of three lines of Matthiola incana is described. Protoplasts were isolated from leaves of 21–28 days old Matthiola plants grown in controlled environment. Sustained divisions were achieved when protoplasts were embedded in sodium alginate. Up to 2.0 % of the protoplasts developed into colonies which could be transferred to shoot regeneration media. More than 25 % of the obtained calluses regenerated shoots. About 4 % of these shoots could be rooted and after transfer to soil phenotypically normal plants have been obtained.

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Abbreviations

2,4-D:

2,4-dichlorphenoxyacetic acid

NAA:

naphthalene acetic acid

IAA:

indole-3-acetic acid

BAP:

6-banzylaminopurine

IPA:

isopentenyladenine

IPAR:

isopentenyladenosine

MES:

(2-[N-morpholino]) ethanesulfonic acid

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Author information

Correspondence to María Dolores Sacristán.

Additional information

Communicated by I. Potrykus

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Siemens, J., Sacristán, M.D. Plant regeneration from mesophyll protoplasts of Matthiola incana (L.) R. Br.. Plant Cell Reports 14, 446–449 (1995). https://doi.org/10.1007/BF00234052

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Keywords

  • Sodium
  • Plant Regeneration
  • Control Environment
  • Sodium Alginate
  • Regeneration Medium