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Nuclear uptake and retention of androgen by the pituitary gland of the hamster and the rat

Summary

Castrated adult male hamsters and castrated adult female rats were injected with either 0.2 μg (hamsters) or 0.5 μg (rats) of 3H-dihydrotestosterone (107 Ci/mmole)/100 grams body weight and killed 11/2 h later. The pituitary glands were removed and processed for both autoradiography and immunocytochemistry (hamster) or only autoradiography (rats). Localization of the androgen was found in 10–15% of the cells of the pars distalis in both species. Only cells that stained for luteinizing hormone (LH) in the hamster's pars distalis concentrated the androgen. Also cells in both the pars intermedia and pars nervosa (1–5%) concentrated the androgen in both species. Although the number of cells that concentrated the androgen in the pars intermedia and pars nervosa was small, this finding may be related to recent physiologic data that suggest that the gonadal steroids may play a role in regulating water retention and natriuresis.

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References

  1. Bogdanove, E.M.: Hypothalamic-hypophyseal interrelationships; basic aspects. In: “Reproductive Biology”, (H. Balin and S. Glasser, eds.). Excerpta Medica, Amsterdam, 5–70 (1972)

  2. Buhl, A.E., Norman, R.L., Resko, S.A.P.: Sex differences in estrogen-induced gonadotropin release in hamsters. Biology of Reproduction, 18, 592–597 (1978)

  3. Erlandsen, S.L., Parsons, J.A., Burke, J.P., Redick, J.A., Van Orden, D.E., Van Orden, L.S.: A modification of the unlabeled antibody enzyme method using heterologous antisera for the light microscopic and ultrastructural localization of insulin, glucagon, and growth hormone. J. Histochem. Cytochem., 23, 666–677 (1975)

  4. Goldman, B.D., Porter, J.C.: Serum LH levels in intact and castrated golden hamsters. Endocrinology 87, 676–679 (1970)

  5. Herbert, D.C.: Identification of the LH and TSH-secreting cells in the pituitary gland of the Rhesus monkey. Cell Tissue Res. 190, 151–161 (1978)

  6. Keefer, D.A., Stumpf, W.E., Petrusz, P.: Quantitative autoradiographic assessment of 3H-estradiol uptake in immunocytochemically characterized pituitary cells. Cell Tissue Res. 166, 25–35 (1976)

  7. Knobil, E.: On the control of gonadotropin secretion in the Rhesus monkey. Rec. Prog. Horm. Res. 30, 1–46 (1970)

  8. Naftolin, F., Ryan, K.J.: the metabolism of androgens in the central neuroendocrine tissue. J. Steroid Biochem. 6, 993–997 (1975)

  9. Sar, M., Stumpf, W.E.: Pituitary gonadotrophs: nuclear concentration of radioactivity after injection of [3H] testosterone. Science 179, 389–391 (1973)

  10. Schally, A.V., Kastin, A.J., Arimura, A.: Effect of sex steroids on pituitary responses to LH and FSH-releasing hormone in vitro. Endocrinology 93, 893–902 (1972)

  11. Sheridan, P.J.: The pars nervosa is a target site for androgens in the mouse. Biol. Reprod. 19, 657–660 (1978)

  12. Sheridan, P.J., Buchanan, J.M.: The effects of opiates on androgen binding in the forebrain of the rat. Int. J. Fert. (in press)

  13. Skowsky, W.R., Swan, L., Smith, P.: Effects of sex steroid hormones on arginine vasopressin in intact and castrated male and female rats. Endocrinology 104, 105–108 (1979)

  14. Sternberger, L.A., Hardy, P.H., Cuculis, J.J., Meyer, H.G.: Preparation and properties of soluble antigen-antibody complex (horseradish peroxidase-antihorseradish peroxidase) and its use in identification of spirochetes. J. Histochem. Cytochem. 18, 315–333 (1970)

  15. Stumpf, W.E.: Autoradiographic techniques for the localization of hormones and drugs at the cellular and subcellular level. Acta Endocrinol. [Kbh.] (suppl.) 153, 205–221 (1971)

  16. Stumpf, W.E., Sar, M.: Autoradiographic localization of estrogen, androgen, progestin, and glucocorticosteroid in “target tissue” and “nontarget tissue”. In: “Receptors and Mechanism of Action of Steroid Hormones”, Part I, (J.R. Pasqualini, ed.) 41–84. New York: Marcel Dekker, Inc., (1976)

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Author information

Correspondence to Dr. P. J. Sheridan.

Additional information

This study was supported by USPHS Research Career Development Award KO4NS0000164 (P.J. Sheridan) and USPHS Grants No. 1 RO1 NS12933, P30 HD10202 and HD 10914

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Sheridan, P.J., Herbert, D.C. Nuclear uptake and retention of androgen by the pituitary gland of the hamster and the rat. Cell Tissue Res. 206, 35–40 (1980). https://doi.org/10.1007/BF00233605

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Key words

  • Androgen receptors
  • Pars nervosa
  • Pars intermedia
  • Autoradiography