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Phosphorylation modulates the voltage dependence of channels reconstituted from the major intrinsic protein of lens fiber membranes

Summary

Major intrinsic polypeptide (MIP), a 28-kDa protein isolated from lens fiber cell membranes, forms large, nonselective channels when reconstituted into lipid bilayers. MIP channels are regulated by voltage, such that these channels close when the potential across the membrane is greater than 30 mV. We have investigated the modulation of the voltage-dependent closure of MIP channels by phosphorylation. In this report, we describe the isolation of two isomers of MIP from lens fiber cell membranes. These isomers differ by a single phosphate at a protein kinase A phosphorylation site. The phosphorylated isomer produces channels that close in response to applied voltages when reconstituted into bilayers. The nonphosphorylated isomer produces voltage-independent hannels. Direct phosphorylation with protein kinase A converts voltage-independent channels to voltage-dependent channels in situ. Analyses of macroscopic and single channel currents suggest that phosphorylation increases the voltage-dependent closure of MIP channels by increasing closed channel lifetimes and the rate of channel closure following the application of voltage.

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Additional information

The authors gratefully acknowledge the gift of the monoclonal antibody to MIP from Drs. David Paul and Dan Goodenough. We thank Dr. Irwin Levitan for the kind gift of purified protein kinase A catalytic subunit. We also thank Ms. Mary Hawley for invaluable technical support and Mr. Paul Ross for help in generating Fig. 10. This work was supported, in part, by NIH grants EY04110 and EY05661 and a NEI postdoctoral fellowship to GRE.

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Ehring, G.R., Lagos, N., Zampighi, G.A. et al. Phosphorylation modulates the voltage dependence of channels reconstituted from the major intrinsic protein of lens fiber membranes. J. Membarin Biol. 126, 75–88 (1992). https://doi.org/10.1007/BF00233462

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Key Words

  • ion channels
  • phosphorylation
  • modulation of ion channels
  • lens fibers
  • reconstitution
  • intercellular junctions
  • major intrinsic protein
  • gap junctions