Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Methyl jasmonate-induced rosmarinic acid biosynthesis in Lithospermum erythrorhizon cell suspension cultures


A dramatic increase in rosmarinic acid (RA) content in cultured cells of Lithospermum erythrorhizon was observed after their exposure to methyl jasmonate (MJ). Preceding the induced RA accumulation, phenylalanine ammonia-lyase (PAL) and 4-hydroxyphenylpyruvate reductase (HPR) activities increased rapidly and transiently, whereas tyrosine aminotransferase (TAT) activity showed only a slight increase. The elicitation activity of MJ was much higher than that of yeast extract (YE) in terms of the induction of PAL and HPR activities, RA accumulation and incorporation of both 14C-phenylalanine and 14C-tyrosine into RA. However, the response of the cultured cells to MJ-treatment was slower than that to YE-treatment.

This is a preview of subscription content, log in to check access.



2,4-dichlorophenoxyacetic acid


Linsmaier and Skoog


4-hydroxyphenylpyruvate reductase


phenylalanine ammonia-lyase


tyrosine aminotransferase


methyl jasmonate


yeast extract


  1. Bradford MM (1976) Anal. Biochem. 72: 248–254

  2. De-Eknamukul W, Ellis BE (1987) Phytochemistry 30: 2877–2881

  3. Ellis BE, Towers GHN (1970) Biochem. J. 118: 287–291

  4. Farmer EE, Ryan CA (1990) Proc. Natl. Acad. Sci. USA 87: 7713–7716

  5. Franceschi VR, Grimes HD (1991) Proc. Natl. Acad. Sci. USA 88: 6745–6749

  6. Gundlach H, Müller MJ, Kutchan TM, Zenk MH (1992) Proc. Natl. Acad. Sci. USA 89: 2389–2393

  7. Häusler E, Petersen M, Alfermann AW (1991) Z. Naturforsch. 46C: 371–376

  8. Linsmaier EM, Skoog F (1965) Physiol. Plant. 18: 100–127

  9. Mizukami H, Ellis BE (1991) Plant Cell Rep. 10: 321–324

  10. Mizukami H, Ogawa T, Ohashi H, Ellis BE (1992) Plant Cell Rep. 11: 480–483

  11. Nojiri H, Yamane H, Seto H, Yamaguchi I, Murofushi N, Yoshihara T, Shibaoka H (1992) Plant Cell Physiol. 33: 1225–1231

  12. Petersen MS (1991) Phytochemistry 30: 2877–2881

  13. Petersen M, Dombrowski K, Gertlowski C, Häusler E, Karwatzki B, Meihard J, Alfermann AW (1992) In: Oono K, Hirabayashi T, Kikuchi S, Handa H, Kajiwara K (ed.) Plant tissue culture and gene manipulation for breeding and formation of phytochemicals, National Institute of Agrobiological Resources, Tsukuba, pp. 297–310

  14. Staswick PE (1992) Plant Physiol. 99: 804–807

  15. Sumaryono W, Proksch P, Hartmann T, Nimitz M, Wray V (1991) Phytochemistry 30: 3267–3271

  16. Weiler EW, Albrecht T, Groth B, Xia Z-Q, Luxem M, Liss H, Andert L, Spengler P (1993) Phytochemistry 32: 591–600

Download references

Author information

Additional information

Communicated by F. Constabel

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Mizukami, H., Tabira, Y. & Ellis, B.E. Methyl jasmonate-induced rosmarinic acid biosynthesis in Lithospermum erythrorhizon cell suspension cultures. Plant Cell Reports 12, 706–709 (1993).

Download citation

Key words

  • Rosmarinic acid
  • Biosynthesis
  • Elicitation
  • Methyl jasmonate
  • Lithospermum erythrorhizon