Embryogenic calli were obtained from a hybrid line of Sorghum bicolor (L.) Moench anthers with mid to late uninucleate pollen cultured in N6 medium supplemented with 3.0% sucrose, 2.0 mg/1 2,4-D, 0.8% agar and incubated at 30°C, which was the optimum temperature. The regeneration of embryos was obtained from the embryogenic calli cultured in modified MS medium supplemented with 3.0% sucrose, 2.0 mg/1 BAP combined with 0.3 mg/1 IAA and 0.8% agar. A total of 248 doubled haploids and 12 haploid plants were regenerated. In a subsequent field study, the selfed progeny from anther culture (designated as the anther culture-2, [A2] generation) derived families was compared with both the F2 and the F1 for agronomic and morphological traits. Significant differences were noticed between the family means of both A2 and F2 for all the quantitative traits studied. The distinctive difference in the behavior of the A2 families in comparison with the F2 was established by within family variance, which was significant in F2 for days to 50 per cent flowering, plant height, panicle length, leaf area index, dry matter production, harvest index and grain yield and was non-significant in A2. Male sterility, one of the potentially important traits, currently exploited in the hybrid seed production of cereals, including CSH5 hybrid sorghum and the morphological traits (panicle shape, compactness, grain color, glume color and nature of the leaf sheath) segregated in the F2. Such segregation was not observed within A2 families and they bred true to their respective A1 plants, indicating the rapid attainment of homozygosity/uniformity. The present study establishes the gametophytic origin of anther culture derived families and indicates the possibility of rapid production of homozygous lines which can be used as recombinant inbreds.
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- N6 :
Chu et al. (1975)
Murashige and Skoog (1962)
- 2,4-D, 2:
Indole-3 -acetic acid
- A1 :
selfed progeny from A1
Chu CC, Wang CC, Sun CS, Chen H, Yin KC, Chu CY, Bi FY (1975) Sci Sinica 18:659–668
Dieu P, Beckert M (1986) Maydica XXXI:245–259
Foroughi WB, Wenzel G (1990) Theor Appl Genet 80:564–568
Kuhlmann U, Foroughi WB (1989) Plant Cell Rep 8:78–81
Liang GH, Xu A, Tang H (1987) Crop Sci 27:336–339
Murashige T, Skoog F (1962) Physiol Plant 15:473–497
Paka K, Widholm JM (1984) Plant Mol Biol Rep 2:37–42
Raina SK (1989) Adv Agron 42:339–397
Rose JB, Dunwell JM, Sunderland N (1986) Plant Cell Tissue Organ Culture 6:15–22
Smith RH, Bhaskaran S (1986) In: Biotechnology in Agriculture and Forestry 2, Crops 1 (ed. Bajaj YPS). Springer Verlag, New York pp. 220–233
Wen FS, Sorensen EL, Barnett FL, Liang GH (1991) Euphytica 52:177–181
Zhang J-M (1982) Acta Bot Yunnan 4:77–82
Communicated by J. M. Widholm
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Kumaravadivel, N., Sree Rangasamy, S.R. Plant regeneration from sorghum anther cultures and field evaluation of progeny. Plant Cell Reports 13, 286–290 (1994). https://doi.org/10.1007/BF00233321
- Anther Culture
- Harvest Index
- Recombinant Inbred
- Haploid Plant
- Panicle Length