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Effects of reserpine and p-chlorophenylalanine on the circadian rhythm of granulated vesicles in the pinealocytes of mice

Summary

The number of granulated vesicles in mouse pinealocytes exhibit a distinct circadian rhythm which is abolished by superior cervical ganglionectomy. Since melatonin treatment markedly affects the number of pinealocytic granulated vesicles, it was suggested that a relationship may exist between norepinephrine, melatonin, and the synthesis and/or secretion of pinealocyte granulated vesicles. The present study was undertaken in an attempt to clarify this relationship. Mice were housed in an environmental chamber under controlled lighting (12L/12D), and were treated with either reserpine, a drug which depletes serotonin and norepinephrine, or p-chlorophenylalanine (p-CPA), an inhibitor of serotonin synthesis. They were sacrificed at various times over a twenty-four hour period, and granulated vesicles present in the pinealocytes were quantitated in thin cross sections through pericapillary areas. Reserpine treatment resulted in a marked increase in the number of granulated vesicles during the dark, but led to a slight decrease during the photoperiod. Treatment with p-CPA produced no significant effect. The results of this study do not support our previous theory that melatonin plays an important role in the regulation of the pinealocyte secretory process, but instead points more directly to the significant role that norepinephrine has in controlling pinealocyte secretion.

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References

  1. Axelrod, J.: The pineal gland: A neurochemical transducer. Science 184, 1341–1348 (1974)

  2. Axelrod, J., Wurtman, R.J., Snyder, S.H.: Control of hydroxyindole-O-methyltransferase activity in the rat pineal gland by environmental lighting. J. Biol. Chem. 240, 949–954 (1965)

  3. Benson, B., Krasovich, M.: Circadian rhythm in the number of granulated vesicles in the pinealocytes of mice. Effects of sympathectomy and melatonin treatment. Cell Tissue Res. 184, 499–506 (1977)

  4. Benson, B., Matthews, M.J., Hruby, V.J.: Characterization and effects of a bovine pineal antigonadotropic peptide. Amer. Zool 16, 17–24 (1976)

  5. Bloom, F.E., Giarman, N.J.: The effects of p-Cl-phenylalanine on the content and cellular distribution of 5-hydroxytryptamine in the rat pineal gland: Combined biochemical and electron microscopic analysis. Biochem. Pharmacol. 19, 1213–1219 (1970)

  6. Brownstein, M., Axelrod, J.: Pineal gland: 24-hour rhythm in norepinephrine turnover. Science 184, 163–165 (1974)

  7. Cardinali, D.P.: Current topics in experimental endocrinology, Vol. II, p. 107. New York: Academic Press 1974

  8. Clabough, J.W.: Ultrastructural features of the pineal gland in normal and light deprived golden hamsters. Z. Zellforsch. 114, 151–164 (1971)

  9. Duffy, D.E., Markesberry, W.R.: Granulated vesicles in sympathetic nerve endings in the pineal gland: Observations on the effects of pharmacologic agents by electron microscopy. Am. J. Anat. 128, 97–116 (1970)

  10. Ebels, I., Benson, B., Matthews, M.J.: Localization of a sheep pineal antigonadotropin. Anal. Biochem. 56, 546–565 (1973)

  11. Fiske, V.M., Bryant, G.K., Putnam, J.: Effect of light on the weight of the pineal in the rat. Endocrinology 66, 489–491 (1960)

  12. Freire, F., Cardinali, D.P.: Effects of melatonin treatment and environmental lighting on the ultrastructural appearance, melatonin synthesis, norepinephrine turnover and microtubule protein content of the rat pineal gland. J. Neural. Transm. 37, 237–257 (1975)

  13. Ito, T., Matsushima, S.: Electron microscopic observations on the mouse pineal, with particular emphasis on its secretory nature. Arch. Histol. Jpn. 30, 1–15 (1968)

  14. Jaim-Etcheverry, J.G., Zieher, L.M.: Cytochemistry of 5-hydroxytryptamine at the electron microscope level. II. Localization in the autonomic nerves of the rat pineal gland. Z. Zellforsch, 86, 393–400 (1968)

  15. Jaim-Etcheverry, G., Zieher, L.M.: Ultrastructural cytochemistry and pharmacology of 5-hydroxytryptamine in adrenergic nerve endings. III. Selective increase of norepinephrine in the rat pineal gland consecutive to depletion of neuronal 5-hydroxytryptamine. J. Pharmacol. Exp. Ther. 178, 42–48 (1971)

  16. Jequier, E., Lovenberg, W., Sjoerdsma, A.: Tryptophan hydroxylase inhibition: The mechanism by which p-CPA depletes rat brain serotonin. Mol. Pharmacol. 3, 274–278 (1967)

  17. Kachi, T., Matsushima, S., Ito, T.: Diurnal changes in glycogen content in the pineal cells of the male mouse. Z. Zellforsch. 118, 310–314 (1971)

  18. Karasek, M.: Ultrastructure of rat pineal gland culture: Influence of norepinephrine, dibutryl cyclic adenosine 3′,5′-monophosphate and adenohypophysis. Endokrinologie 64, 106–114 (1974)

  19. Klein, D.C., Weller, J.L.: Indole metabolism in the pineal gland: A circadian rhythm in N-acetyltransferase. Science 169, 1093 (1970)

  20. Klein, D.C., Weller, J.L.: Pineal N-acetylserotonin. Fed. Proc. 31, 222 (1972)

  21. Klein, D.C., Weller, J.L., Moore, R.Y.: Melatonin metabolism: Neural regulation of pineal serotonin: acetyl coenzyme A N-acetyltransferase activity. Proc. Natl. Acad. Sci. 68, 3107–3110 (1971)

  22. Koe, B.K., Weissman, A.: p-Chlorophenylalanine: A specific depletor of brain serotonin. J. Pharmacol. Exp. Ther. 154, 499–516 (1966)

  23. Lu, K.S., Lin, H.S.: Cytochemical studies on cytoplasmic granular elements in the hamster pineal gland. Histochemistry 61, 177–187 (1979)

  24. Lukaszyk, A., Reiter, R.: Histophysiological evidence for the secretion of polypeptides by the pineal gland. Am. J. Anat. 143, 451–464 (1975)

  25. Lynch, H.J.: Diurnal oscillations in pineal melatonin content. Life Sci. 10, 791–795 (1971)

  26. Matsushima, S., Ito, T.: Diurnal changes in sympathetic nerve endings in the mouse pineal: Semiquantitative electron microscopic observations. J. Neural. Transm. 33, 275–288 (1972)

  27. Matsushima, S., Morisawa, Y., Mukai, S.: Diurnal variation in large granulated vesicles in sympathetic nerve fibers of the mouse pineal-quantitative electron microscopic observations. J. Neural. Transm. 45, 63–73 (1979)

  28. Morgan, W.W., Reiter, R.J.: Pineal noradrenaline levels in the Mongolian Gerbil and in different strains of laboratory rats over a lighting regimen. Life Sci. 21, 555–558 (1977)

  29. Nir, I., Hirschmann, N., Mishkinsky, J., Sulman, F.G.: The effect of light and darkness on nucleic acids and protein metabolism of the pineal gland. Life Sci. 8, 279–287 (1969)

  30. Nir, I., Hirschmann, N., Sulman, F.G.: Diurnal rhythms of pineal nucleic acids and proteins. Neuroendocrinology 7, 271–277 (1971)

  31. Nunez, E.A., Gershon, M.D.: Synthesis and storage of serotonin by parafollicular (C) cells of the thyroid gland of active, prehibernating and hibernating bats. Endocrinology 90, 1008–1024 (1972)

  32. Pavel, S., Dumitru, I., Klepsch, I.: A gonadotropin inhibiting principle in the pineal of human fetuses. Evidence for its identity with arginine vasotocin. Neuroendocrinology 13, 41–46 (1973/74)

  33. Pellegrino de Iraldi, A.: Granulated vesicles in the pineal gland of the mouse. Z. Zellforsch. 101, 408–418 (1969)

  34. Pellegrino de Iraldi, A., Gueudet, R.: Action of reserpine on the osmium tetroxide zinc iodide reactive site of synaptic vesicles in the pineal nerves of the rat. Z. Zellforsch. 91, 178–185 (1968)

  35. Pevet, P., Ariëns Kappers, J., Nevo, E.: The pineal gland of the mole rat (Spalax ehrenbergi, Nehring). Cell Tissue Res. 174, 1–24 (1976)

  36. Quay, W.B.: Reduction of mammalian pineal weight and lipid during continuous light. Gen. Comp. Endocrinol. 1, 211–217 (1961)

  37. Quay, W.B.: Circadian rhythm in rat pineal serotonin and its modifications by estrous cycle and photoperiod. Gen. Comp. Endocrinol. 3, 473–479 (1963)

  38. Quay, W.B.: Circadian and estrous rhythms in pineal melatonin and 5-hydroxy-indole-3-acetic acid. Proc. Soc. Exp. Biol. Med. 115, 710–713 (1964)

  39. Quay, W.B.: Photic relations and experimental dissociation of circadian rhythms in pineal composition and running activity in rats. Photochem. Photobiol. 4, 425–432 (1965)

  40. Reynolds, E.S.: The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17, 208–212 (1963)

  41. Romijn, H.J.: The ultrastructure of the rabbit pineal gland after sympathectomy, parasympathectomy, continuous illumination and continuous darkness. J. Neural. Transm. 36, 183–194 (1975)

  42. Romijn, H.J., Gelsema, A.J.: Electron microscopy of the rabbit pineal organ in vitro. Evidence of norepinephrine-stimulated activity of the Golgi apparatus. Cell Tissue Res. 172, 365–377 (1976)

  43. Romijn, H.J., Mud, M.T., Wolters, P.S.: Diurnal variations in number of Golgi-dense-core vesicles in light pinealocytes of the rabbit. J. Neural. Transm. 38, 231–237 (1976)

  44. Romijn, H.J., Mud, M.T., Wolters, P.S.: A pharmacological and autoradiographic study on the ultrastructural localization of indoleamine synthesis in the rabbit pineal gland. Cell Tissue Res. 185, 199–214 (1977)

  45. Roth, W.D., Wurtman, R.J., Altschule, M.D.: Morphologic changes in the pineal parenchyma cells of rats exposed to continuous light or darkness. Endocrinology 71, 888–892 (1962)

  46. Shibuya, H., Toru, M., Watanabe, S.: A circadian rhythm of tryptophan hydroxylase in rat pineals. Brain Res. 138, 364–368 (1978)

  47. Snyder, S.H., Axelrod, J.: Circadian rhythm in pineal serotonin: Effect of monoamine oxidase and reserpine. Science 149, 542–544 (1965)

  48. Snyder, S.H., Zweig, M., Axelrod, J., Fischer, J.E.: Control of the circadian rhythm in serotonin content of the rat pineal gland. Proc. Natl. Acad. Sci. 53, 301–305 (1965)

  49. Upson, R.H., Benson, B.: Effects of blinding on the ultrastructure of mouse pinealocytes with particular emphasis on the dense-cored vesicles. Cell Tissue Res. 183, 491–498 (1977)

  50. Upson, R.H., Benson, B., Satterfield, V.: Quantitation of ultrastructural changes in the mouse pineal in response to continuous illumination. Anat. Rec. 184, 311–324 (1976)

  51. Vaughn, M.K., Vaughn, G.M., Klein, D.C.: Arginine vasotocin: Effects on development of reproductive organs. Science 186, 838–839 (1974)

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Author information

Correspondence to Ms. Margaret Krasovich.

Additional information

Supported in part by N.I.H. Grant # HD 08759

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Krasovich, M., Benson, B. Effects of reserpine and p-chlorophenylalanine on the circadian rhythm of granulated vesicles in the pinealocytes of mice. Cell Tissue Res. 203, 457–467 (1979). https://doi.org/10.1007/BF00233274

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Key words

  • Pinealocytes (mouse)
  • Granulated vesicles
  • Circadian rhythm
  • Reserpine
  • p-Chlorophenylalanine (p-CPA)
  • Melatonin