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Antiauxin enhanced microshoot initiation and plant regeneration from epicotyl-originated thin-layer explants of sugarbeet (Beta vulgaris L.)

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Abstract

An in vitro method was developed for microshoot initiation from thin-layer explants prepared from the elongated epicotyls of sugarbeet (Beta vulgaris L.). Intact epicotyls of 14-day-old seedlings were excised from the hypocotyls above the cotyledons and allowed to elongate on De Greef and Jacobs (1979) medium supplemented with 0.2 mg/l 6-benzyladenine, 0.2 mg/l gibberellic acid and 0.1 mg/l indole-3-acetic acid in darkness. After a 21-day-incubation, the elongated epicotyls were halved to obtain apical and basal segments prior to removing the leaves and lateral buds. Subsequently, 5–8 mm long, 2–3 mm wide and 0.8–1.0 mm thick tangential sections were prepared longitudinally from the exterior parts of the halved epicotyls. These thin-layer explants were incubated on microshoot initiating media containing various growth regulators. The combination of 1.0 mg/l 6-benzyladenine and the antiauxin 2,3,5-triiodobenzoic acid (1.0 mg/l) resulted in maximum microshoot development (6.3±0.2 microshoots/thin-layer explant). The final efficiency of our tissue culture system was significantly increased by the NaCl (100 mg/l) initiated in vitro rooting of microshoot originated plantlets.

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Abbreviations

AC:

activated charcoal

asdp :

apical segment derived plantlet

asTLE:

apical segment derived thin-layer explant

BA-6:

benzyladenine

bsdp :

basal segment derived plantlet

bsTLE:

basal segment derived thin-layer explant

EEM1-4:

epicotyl elongation media

GA3 :

gibberellic acid

GM:

germinating medium

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

KN:

kinetin

MES:

morpholino-ethanesulfonic acid

MSI1-6:

microshoot initiating media

NAA:

α-naphthalene acetic acid

PGoB :

De Greef and Jacobs (1979) medium

RM1-3:

rooting media

SDM:

shoot developing medium

SE:

standard error

TIBA 2,3,5:

triiodobenzoic acid

TLE:

thin-layer explant

ZEA:

zeatin

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Communicated by H. Lörz

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Toldi, O., Gyulai, G., Kiss, J. et al. Antiauxin enhanced microshoot initiation and plant regeneration from epicotyl-originated thin-layer explants of sugarbeet (Beta vulgaris L.). Plant Cell Reports 15, 851–854 (1996). https://doi.org/10.1007/BF00233155

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Keywords

  • Tissue Culture
  • Growth Regulator
  • Culture System
  • Plant Regeneration
  • Gibberellic Acid