A high frequency shoot regeneration (80%) was developed from callus of leaf discs and stem internodes of Moricandia arvensis. Leaf discs were shown to be a preferable starting material for transformation experiments. Agrobacterium tumefaciens strain GV3101/pMP90 used in this study contained a binary vector with genes for kanamycin resistance, hygromycin resistance and β-glucuronidase (GUS). Maximum transformation efficiency (10.3%) was achieved by using kanamycin at the rate of 200 mg/l as a selection agent. Presence of tobacco suspension culture during co-cultivation and a pre-selection period of seven days after co-cultivation was essential for successful transformation. Transgenic plants grew to maturity and exhibited flowering in a glasshouse. GUS activity was evident in all parts of leaf and the presence of GUS gene in plant gemone was confirmed by PCR analysis.
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An G, Evert PR, Mitra A, Ha SB (1988) In: Gelvin SB, Schilperoort RA (eds) Plant Molecular Biology Manual, Kluwer Academic Publishers, Dordrecht, Netherlands, pp A3/1–19.
Barfield DG, Pua EC (1991) Plant Cell Rep 10: 308–314.
Bruhl JJ, Stone NE, Hattersley PW (1987) Aust J Plant Physiol 14:719–728.
Chitty JA, Furbank RT, Marshall JS, Chen Z, Taylor WC (1994) Plant J 6: 949–956.
De Block M, De Brouwer D, Tenning P (1989) Plant Physiol 91:694–701.
Devi MT, Rajagopalan AV, Raghavendra AS (1995) Plant Cell Environ 18: 589–594.
Hiei Y, Ohta S, Komari T, Kumashiro T (1994) Plant J 6: 271–282.
Holaday AS, Chollet R (1984) Photosynthesis Res 5: 307–323.
Holaday AS, Shieh YJ, Lee KW, Chollet R (1981) Biochim Biophys Acta 637: 334–341.
Hood EE, Helmer GA, Fraley RT, Chilton M (1986) J of Bacter 168: 1291–1301.
Horsch RB, Fry J, Hoffmann N, Neidermeyer J, Rogers SG, Fraley RT (1988) In: Gelvin SB, Schilperoort RA (eds) Plant Molecular Biology Manual, Kluwer Academic Publishers, Dordrecht, Netherlands, pp A5/1-A5/9.
Jefferson RA (1987) Plant Mol Biol Rep 5: 387–405.
Koncz C, Schell J (1986) Mol Gen Genet 204:383–396.
Martineau B, Smith HJ, Dean C, Dunsmir P, Bedbrook J, Metes LJ (1989) Plant Mol Biol 13: 419–426.
Murashige T, Skoog F (1962) Physiol Plant 15: 473–497.
Murray MG, Thompson WF (1980) Nucl Acid Res 8: 4321–432.
Ohta S, Mita S, Hattori T, Nakamura K (1990) Plant Cell Physiol 31: 805–813.
Radke SE, Andrews BM, Moloney MM, Crouch ML, Kridl JC, Knauf VC (1988) Theor Appl Genet 75:685–694.
Rashid H, Yokoi S, Toriyama K, Hinata K (1995) Plant Cell Rep (in press)
Rawsthorne S (1992) Plant J 2:267–274.
Stachel SE, Messens E, Van Montagu M, Zambryski P (1985) Nature 318:624–629.
Toriyama K, Hinata K (1985) Plant Sci 41: 179–183.
Toriyama K, Kameya T, Hinata K (1987a) Plant Tiss Cult Letter 4:75–78.
Toriyama K, Hinata K, Kameya T (1987b) Plant Sci 48:123–128.
Tsuchiya T, Toriyama K, Yoshikawa M, Ejiri S, Hinata K (1995) Plant Cell Physiol 36:487–494.
Valvekens D, Van Montagu M, Van Lijsebettens M (1988) Proc Natl Acad Sci USA 85:5536–5540.
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Rashid, H., Toriyama, K. & Hinata, K. Transgenic plant production from leaf discs of Moricandia arvensis using Agrobacterium tumefaciens . Plant Cell Reports 15, 799–803 (1996). https://doi.org/10.1007/BF00233143
- Agrobacterium tumefaciens
- Leaf disc transformation
- Moricandia arvensis
- Transgenic plants