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Transgenic plant production from leaf discs of Moricandia arvensis using Agrobacterium tumefaciens

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Summary

A high frequency shoot regeneration (80%) was developed from callus of leaf discs and stem internodes of Moricandia arvensis. Leaf discs were shown to be a preferable starting material for transformation experiments. Agrobacterium tumefaciens strain GV3101/pMP90 used in this study contained a binary vector with genes for kanamycin resistance, hygromycin resistance and β-glucuronidase (GUS). Maximum transformation efficiency (10.3%) was achieved by using kanamycin at the rate of 200 mg/l as a selection agent. Presence of tobacco suspension culture during co-cultivation and a pre-selection period of seven days after co-cultivation was essential for successful transformation. Transgenic plants grew to maturity and exhibited flowering in a glasshouse. GUS activity was evident in all parts of leaf and the presence of GUS gene in plant gemone was confirmed by PCR analysis.

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Abbreviations

GUS:

β-glucuronidase

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Communicated by A. Komamine

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Rashid, H., Toriyama, K. & Hinata, K. Transgenic plant production from leaf discs of Moricandia arvensis using Agrobacterium tumefaciens . Plant Cell Reports 15, 799–803 (1996). https://doi.org/10.1007/BF00233143

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Key words

  • Agrobacterium tumefaciens
  • Leaf disc transformation
  • Moricandia arvensis
  • Transgenic plants