Springer Nature is making SARS-CoV-2 and COVID-19 research free View research | View latest news | Sign up for updates

In vitro propagation of Clerodendrum colebrookianum Walp., a potential natural anti-hypertension medicinal plant

  • 95 Accesses

  • 7 Citations


A rapid clonal propagation system for Clerodendrum colebrookianum Walp. (Verbenaceae), a anti-hypertension folk medicinal shrub has been developed. A range of cytokinins has been investigated for multiple shoot induction with shoot apex, axillary shoot, leaf, petiole and root explants. Optimum shoot induction occurred with axillary buds using 6-benzyladenine where an average of 21 shoots were produced per explant in 6 weeks. Subculturing the newly produced shoots, by separating into groups of five shoots, produced an average of 43 new shoots per culture within 4 weeks. In vitro rooting and weaning of over 200 plantlets was completely successful. Cytological studies revealed no visible abnormalities in chromosome number.

This is a preview of subscription content, log in to check access.







Least Significant Difference


1-naphthaleneacetic acid




Woody Plant Medium (Lloyd & McCown 1980) basal medium


  1. Evers PW, Donkers J, Prat A, Vermeer E (1988) Micropropagation of forest trees through tissue culture. Centre for Agricultural Publishing and Documentation (Pudoc), Wageningen

  2. Fauvel MT, Gleye J, Andary C (1989) Plant Med. 55: 577

  3. Goldblatt P (1981) Plant chromosomal index 1976–1978. Braun-Brumfield Inc., Ann Arbor, Michigan

  4. Hildrum H (1972) New York State Flower Ind. Bul. Nov – Dec, pp 3–4

  5. Inagaki N, Nishimura H, Okada M, Mitsuhashi H (1991) Plant Cell Rep 9: 484–487

  6. Karp A, (1991) In: Miflin BJ (Ed) Oxford Surveys in Plant Molecular and Cell Biology vol 7, pp 1–58

  7. Lloyd G, McCown B (1980) Int Plant Prop Soc Proc 30: 421–427

  8. Moldenke HN (1971) A resume of the Verbenacaeae, Stilbaceaceae, Symphoremaceae and Eriocaulaceae of the World as to valid taxa, geographic distribution and synonymy, 1+2, Moldenke, Wayne, New Jersey

  9. Nath SC, Bordoloi DN (1991) Int J Pharm 29: 127–129

  10. Pal S, Choudhury A, Adityachaudhury N (1989) J Agri-Food Chem 37: 234–236

  11. Pradad TK, Mehta PM, Dave YS (1984) Biol Plant 26: 321–326

  12. Sanderson KC, Martin WC Jr, McGuire J (1990) Hort Sci 25: 125

  13. Somasundaram S, Sadique J (1986) Fitoterapia 57: 103–110

  14. Thomas HE (1981) The New York Botanical Garden Illustrated Encyclopedia of Horticultur. Garland Publishing Inc. New York

  15. Toyota M, Msonthi JD, Hostettmann K (1990) Phytochemistry 29: 2849–2851

Download references

Author information

Additional information

Communicated by M.R. Davey

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Mao, A.A., Wetten, A., Fay, M. et al. In vitro propagation of Clerodendrum colebrookianum Walp., a potential natural anti-hypertension medicinal plant. Plant Cell Reports 14, 493–496 (1995). https://doi.org/10.1007/BF00232781

Download citation


  • Medicinal Plant
  • Chromosome Number
  • Propagation System
  • Clonal Propagation
  • Shoot Apex