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An unusual root tip formation in hairy root culture of Hyoscyamus muticus

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Summary

Hairy root cultures of Hyoscyamus muticus were established using Agrobacterium rhizogenes ATCC 15834. In one out of 8 clones established, an unusual root tip formation was observed after transfer of cultures from half-strength Murashige and Skoog (1962) to White's medium (1939). This phenomenon was associated with the production of a fine brownish cell suspension culture. Hairy root development resumed after transfer of the root tips from White to half-strength Murashige and Skoog medium. After plating the isolated brownish cells on hormone-free half-strength Murashige and Skoog or White solid medium, callus proliferation was observed, and then redifferentiation of hairy roots occurred. The polymerase chain reaction analysis of the H. muticus hairy root (clone Z2) revealed that only the tl region of the T-DNA was integrated. The growth and the production of five tropane alkaloids by this clone were examined.

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Abbreviations

PCR:

Polymerase Chain Reaction

MS medium:

Murashige and Skoog Medium

1/2 MS medium:

half-strength MS medium

WP medium:

Woody Plant medium

RC medium:

Root Culture medium

WH medium:

White medium

HPLC:

High Performance Liquid Chromatography

wt.:

weight

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Communicated by A. Komamine

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Jaziri, M., Homes, J. & Shimomura, K. An unusual root tip formation in hairy root culture of Hyoscyamus muticus . Plant Cell Reports 13, 349–352 (1994). https://doi.org/10.1007/BF00232635

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Key words

  • Hyoscyamus muticus
  • Agrobacterium rhizogenes
  • hairy root
  • adventitious root tip
  • tropane alkaloid