The addition of 3 mg/l of nordihydroguaiaretic acid (NDGA) to BAP and tyrosine fortified MS medium was essential to obtain organogenic callus from the hypocotyl segments of two varieties (D-154 and CVL-1) of Corchorus capsularis — one of the two jute species. When the organogenic callus, which is rich in large starch granules, was transferred to MS basal medium, it differentiated into single or multiple shoots usually in the first subculture and sometimes in the second. The activity of glyoxalase-I of the organogenic callus was found to be significantly lower than that observed in the nonorganogenic callus initiated on MS medium supplemented with 2,4-D, tyrosine, BAP or just BAP and tyrosine. This suggests an inverse relationship between differentiation and the level of glyoxalase-I activity in the two varieties of C. capsularis jute.
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- CVL 1:
Corchorus capsularis var. CVL-1
C. capsularis var. D 154
C. olitorius var. O-4
Ahmed G, Hossain ABMM, Islam MS (1989) Indian J. Exp Biols 27: 334–337.
Bayliss MW (1985) In: Byrant JA and Francis D (eds.) The cell Division Cycle in Plants, Cambridge Univ. Press, Cambridge, pp. 157–178.
Das B, Haque MM, Islam AS, Rahman MH, Hoque MI (1986) In: Rao AN and Mohon Ram HY (eds.). Proc. Nayadumma Mem. Symp. on Agricultural Application in Biotechnology, Madras. India, pp. 106–114
Fitch M, Manshardt RM (1990) Plant Cell Rep. 9: 320–324.
Gould AR, Everett NP, Wang TL, Street HE (1981) Protoplasma 106: 1–13.
Islam AS (1981) In: Rao AN (ed.) Proc. COSTED Symposium on Tissue Culture of Economically Important Rants, Singapore, pp. 159–161
Islam MS, Nessa A (1987) Fungal Genet Newsletter 34: 36–37.
Lowry OH, Rosenbrugh NJ, Fars RJ, Randall JJ (1951) J Biol Chem 193: 265–275.
Munkres KD (1979) J Gen Appl Microbiol 25: 323–327.
Murashige T, Skoog F (1962) Physiol Plant 15: 473–497.
Nayak P, Sen SK (1989) Plant Cell Rep 8: 296–299.
Nyandeika HS (1978) Clin Biochem 11: 150–155.
Rahman SMZ, Hadiuzzaman S, Haque MM, Islam AS (1985) Bangladesh J Bot 14: 141–145.
Racker E (1951) J Biol Chem 190: 685–696.
Ramaswamy O, Pal S, Guha-Mukherjee S, Sopory SK (1983) Biochem nternatl 7: 307–318.
Ramaswamy O, Pal S, Guha-Mukherjee S, Sopory SK (1984) Plant Cell Rep 3: 121–124.
Sethi U, Basu A, Guha-Mukherjee S (1988) Plant Sci 56: 167–175.
Communicated by J. M. Widholm
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Seraj, Z.I., Sarker, A.B. & Islam, A.S. Plant regeneration in a jute species (C. capsularis) and its possible relationship with glyoxalase-I. Plant Cell Reports 12, 29–33 (1992). https://doi.org/10.1007/BF00232418
- Basal Medium
- Inverse Relationship
- Plant Regeneration