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Plant regeneration in a jute species (C. capsularis) and its possible relationship with glyoxalase-I

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Summary

The addition of 3 mg/l of nordihydroguaiaretic acid (NDGA) to BAP and tyrosine fortified MS medium was essential to obtain organogenic callus from the hypocotyl segments of two varieties (D-154 and CVL-1) of Corchorus capsularis — one of the two jute species. When the organogenic callus, which is rich in large starch granules, was transferred to MS basal medium, it differentiated into single or multiple shoots usually in the first subculture and sometimes in the second. The activity of glyoxalase-I of the organogenic callus was found to be significantly lower than that observed in the nonorganogenic callus initiated on MS medium supplemented with 2,4-D, tyrosine, BAP or just BAP and tyrosine. This suggests an inverse relationship between differentiation and the level of glyoxalase-I activity in the two varieties of C. capsularis jute.

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Abbreviations

BAP:

6-benzylaminopurine

CVL 1:

Corchorus capsularis var. CVL-1

D-154:

C. capsularis var. D 154

O-4:

C. olitorius var. O-4

2,4-D:

2,4-dichlorophenoxyacetic acid

IBA:

indole-3-butyric acid

NDGA:

nordihydroguaiaretic acid

tyr:

tyrosine

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Communicated by J. M. Widholm

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Seraj, Z.I., Sarker, A.B. & Islam, A.S. Plant regeneration in a jute species (C. capsularis) and its possible relationship with glyoxalase-I. Plant Cell Reports 12, 29–33 (1992). https://doi.org/10.1007/BF00232418

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Keywords

  • Starch
  • Tyrosine
  • Basal Medium
  • Inverse Relationship
  • Plant Regeneration