We have evaluated the immunosuppressive effect of a new stable derivative of cyclophosphamide (ASTA Z 7557) on human lymphocyte immunoglobulin biosynthesis in vitro. When graded amounts of the drug are added to lymphocyte cultures stimulated with the helper T cell-dependent activator (PWM), a marked inhibition of B cell proliferation and differentiation occurs. Lymph node cells are particularly sensitive to the drug, while splenocytes are relatively resistant. The agent exerts only a minor effect on immunoglobulin synthesis triggered by a direct B cell stimulant (S. paratyphi bacteria).
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Gorski A, Korczak G, Wasik M, Nowaczyk M, Podobinska I, Malejczyk M, Gaciong Z, Paczek L: Mode of action of cyclophosphamide as assessed by in vitro activity of its active metabolite. Transplantation Proc 15:573, 1983
Gorski A, Nowaczyk M, Rowinski W: Immunomodulatory influence of active metabolite of cyclophosphamide on human lymphocyte immunoglobulin biosynthesis in vitro. Transplantation 35:577, 1983
Gorski A, Korczak-Kowalska G, Chorzelski T: Intracellular localization of synthesized immunoglobulins in B cells: a modified method. J Immunol Methods 57:33, 1983
Pohl J, Hilgard P: Experimental toxicology of ASTA Z 7557. Invest N Drugs 2:2, 1984
Bird AG, McLachlan SM, Britton S: Cyclosporin A promotes spontaneous outgrowth in vitro of Epstein-Barr virus-induced B-cell lines. Nature 289:300, 1981
About this article
Cite this article
Gorski, A., Korczak-Kowalska, G. Inhibition of human B lymphocyte differentiation by a stable metabolite of cyclophosphamide (ASTA Z 7557, INN mafosfamide). Invest New Drugs 2, 227–229 (1984). https://doi.org/10.1007/BF00232356
- ASTA Z 7557
- Ig synthesis
- B lymphocytes