Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Induction of pollen callus in anther cultures of Feijoa sellowiana Berg. (Myrtaceae)


Anthers of Feijoa sellowiana Berg. (feijoa) produced pollen callus when cultured in Murashige and Skoog medium containing 2,4-dichlorophenoxyacetic acid and benzyladenine or in nurse cultures. Somatic callus was also formed in large amounts from the connective and from the cut end of the filament. Anthers containing microspores at the stage immediately prior to the first pollen mitosis cultured in the presence of 3% sucrose, presented the highest frequencies of induction. Androgenetic divisions were initiated by the formation of two morphologically equal cells, the so-called B-pathway. Attempts to regenerate pollen plants were unsuccessful but leaf-like structures could be obtained in regeneration media containing combinations of gibberellic acid and benzyladenine.

This is a preview of subscription content, log in to check access.



2,4-diclorophenoxyacetic acid




fluorescein diacetate

GA3 :

gibberellic acid




Murashige and Skoog (1962) medium


  1. Arnold SV, Eriksson T (1981) In vitro studies of adventitious shoot formation in Pinus conforta. Can. J. Bot. 59:870–874

  2. Babbar SB, Gupta SC (1986) Induction of androgenesis and callus formation in in vitro cultured anthers of a myrtaceous fruit tree (Psidium guajava L.). Bot. Mag. Tokyo 99:75–83

  3. Bhojwani SS, Dhawan V, Arora R (1990) Plant tissue culture a classified bibliography 1985–1989. Elsevier, Amsterdam

  4. Canhoto JM, Guimarães MLS, Cruz GS (1990) In vitro induction of haploid, diploid and triploid plantlets by anther culture of Iochroma warscewiczii Regel. Plant Cell, Tissue and Organ Culture 21:171–177

  5. Chen CH, Chen FT, Chien CF, Wang CH, Chang SC, Hsu HE, Ho YT, Lu TM (1979) A process of obtaining pollen plants of Hevea brasiliensis Muell.-Arg. Scientia Sinica 22:81–90

  6. Chen Z (1986) Induction of androgenesis in woody plants. In: Hu H, Yang H (eds) Haploids in higher plants. Springer-Verlag, Berlin, pp 42–66

  7. Cruz GS, Canhoto JM, Abreu MAV (1990) Somatic embryogenesis and plant regeneration from zygotic embryos of Feijoa sellowiana Berg. Plant Science 66:263–270

  8. Guha S, Maheshwari SC (1964) In vitro production of embryos from anthers of Datura. Nature (London) 204:497

  9. Gonçalves AT, Machado MA, Caldas LS, Sharp WR, Mello HDA (1979) Tissue culture of Eucalyptus. In: Sharp WR et al (eds) Plant cell and tissue culture: principles and applications. Ohio State Univ. Press, Columbus, pp 509–526

  10. Lin S, JI D, Qin J (1987) In vitro production of haploid plants from mulberry (Morus) anther culture. Scientia Sinica 30: 853–863

  11. McCown BM, Lloyd G (1981) Woody plant medium (WPM).A mineral nutrient formulation for microculture of woody plant species. HortScience 16:89

  12. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473–497

  13. Nair S, Gupta PK, Mascarenhas AF (1983) Haploid plants from in vitro anther culture of Annona squamosa. Plant Cell Reports 2:198–200

  14. Nitsch JP, Nitsch C (1969) Haploid plants from pollen grains. Science 163: 85–87

  15. Radojevic L (1978) In vitro induction of androgenic plants in Aesculus hippocastanum. Protoplasma 96:369–374

  16. Redenbaugh MK, Westgall RD, Karnosky DF (1981) Dihaploid callus production from Ulmus americana anthers. Bot. Gaz. (Chicago) 142:19–26

  17. Sharp WR, Raskin RS, Sommer HE (1972) The use of nurse culture in the development of haploid clones in tomato. Planta 104:357–361

  18. Sunderland N, Dunwell, JM (1974) Pathways in pollen embryogenesis. In: Street HE (ed) Tissue culture and plant science. Academic Press, London, pp 141–167

  19. Zenkteler M, Stefaniak B (1982) Induction of androgenesis in anthers of Hordeum vulgare L.cultured in vitro on leaves and calluses. Plant Science Lett. 26:219–225

Download references

Author information

Additional information

Communicated by G. Pelletier

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Canhoto, J.M., Cruz, G.S. Induction of pollen callus in anther cultures of Feijoa sellowiana Berg. (Myrtaceae). Plant Cell Reports 13, 45–48 (1993). https://doi.org/10.1007/BF00232314

Download citation


  • Feijoa sellowiana
  • Pollen callus
  • B-pathway