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Induction of pollen callus in anther cultures of Feijoa sellowiana Berg. (Myrtaceae)

Summary

Anthers of Feijoa sellowiana Berg. (feijoa) produced pollen callus when cultured in Murashige and Skoog medium containing 2,4-dichlorophenoxyacetic acid and benzyladenine or in nurse cultures. Somatic callus was also formed in large amounts from the connective and from the cut end of the filament. Anthers containing microspores at the stage immediately prior to the first pollen mitosis cultured in the presence of 3% sucrose, presented the highest frequencies of induction. Androgenetic divisions were initiated by the formation of two morphologically equal cells, the so-called B-pathway. Attempts to regenerate pollen plants were unsuccessful but leaf-like structures could be obtained in regeneration media containing combinations of gibberellic acid and benzyladenine.

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Abbreviations

2,4-D:

2,4-diclorophenoxyacetic acid

BA:

benzyladenine

FDA:

fluorescein diacetate

GA3 :

gibberellic acid

Kn:

kinetin

MS:

Murashige and Skoog (1962) medium

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Communicated by G. Pelletier

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Canhoto, J.M., Cruz, G.S. Induction of pollen callus in anther cultures of Feijoa sellowiana Berg. (Myrtaceae). Plant Cell Reports 13, 45–48 (1993). https://doi.org/10.1007/BF00232314

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Keywords

  • Feijoa sellowiana
  • Pollen callus
  • B-pathway