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The use of transient GUS expression to develop an Agrobacterium-mediated gene transfer system for kiwifruit


We have monitored transient GUS expression 4–5 days after cocultivation of leaf explants with Agrobacterium, in order to optimise parameters of cocultivation and so develop an efficient, reproducible gene transfer system in kiwifruit. Factors that were important included the health of the explant, the strain of Agrobacterium, and the binary vector used. Pre-culture of the leaf explants before cocultivation inhibited gene transfer at the cut edge. Placing the explants on moist filter paper during cocultivation gave increased frequencies of gene transfer. Stably transformed, kanamycin-resistant plants were obtained at good frequency from the optimised system. PCR and Southern analysis of the regenerated plants confirmed their transgenic nature.

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  1. An G, Ebert PR, Mitra A, Ha SB (1988) Binary vectors. In “Plant molecular biology manual”, Kluwer Academic Publishers, Dordrecht, A3: 1–19.

  2. Atkinson RG, Candy CJ, Gardner RC (1990) New Zealand J. Crop Hort. Sci. 18: 153–156

  3. Atkinson RG, Gardner RC (1991) Plant Cell Rep.: 10: 208–212

  4. Atkinson RG and Gardner RC (1993) Plant Cell Rep. 12: 347–351

  5. Candy CJ (1987) MSc thesis, University of Auckland, New Zealand

  6. Crowhurst RN (1990) PhD Thesis, University of Auckland, NewZealand

  7. Dong J-Z, McHughen A (1991) Plant Cell Rep. 10: 555–560

  8. Fillatti JJ, Kiser J, Rose R, Comai L (1987a) Bio/Tech. 5: 726–730

  9. Fillatti JJ, Sellmer J, McCown B, Haissig B, Comai L (1987b) Mol. Gen. Genet. 206: 192–199

  10. Fraley RT, Horsch RB, Matzke A, Chilton M-D, Chilton WS, Sanders PR (1984) Plant Mol. Biol. 3: 371–378

  11. Higgens ES, Hulme JS, Shields R (1992) Plant Science 82: 109–118

  12. Hoekema A, Hirsch PR, Hooykaas PJJ, Schilperoort RA (1983) Nature 303: 179–180

  13. Hood EE, Helmer GL, Fraley RT, Chilton M-D (1986) J. Bacteriol. 168: 1291–1301

  14. Horsch RB, Fry JE, Hoffmann NL, Eichholtz D, Rogers SG, Fraley RT (1985) Science 227: 1229–1231

  15. Janssen BJ (1991) PhD Thesis, University of Auckland, New Zealand

  16. Janssen B-J, Gardner RC (1990) Plant Mol.Biol. 14: 61–72

  17. Ledger SE, Deroles SC, Given NK (1991) Plant Cell Rep. 10: 195–199

  18. Li X-Q, Liu C-N, Ritchie SW, Peng J, Gelvin SB, Hodges TK (1992) Plant Mol.Biol. 20: 1037–1048

  19. Lu C-Y, Nugent G, Wardley-Richardson T, Chandler SF, Young R, Dalling MJ (1991) Bio/Tech. 9: 864–868

  20. McCormick S, Niedermeyer J, Fry J, Barnason A, Horsch R, Fraley R (1986) Plant Cell Rep. 5: 81–84

  21. Mullins MG, Tang FCA, Facciotti D (1990) Bio/Tech. 8: 1041–1045

  22. Murashige T, Skoog F (1962) Physiol. plant. 15: 473–497

  23. Murray MG, Thompson WF (1980) Nucl. Acids Res. 8: 4321–4325

  24. Nehra NS, Chibbar RN, Kartha KK, Datla RSS, Crosby WL, Stushnoff C (1990) Plant Cell Rep. 9: 293–298

  25. Rained DM, Bottino P, Gordon MP, Nester EW (1990) Bio/Tech. 8: 33–38

  26. Rogers SG, Klee HJ, Horsch RB, Fraley RT (1987) Methods in Enzymology 153: 253–277

  27. Rugini E, Pellegrineschi A, Mencuccini M, Mariotti D (1991) Plant Cell Rep. 10: 291–295

  28. Schmidt R, Willmitzer L (1988) Plant Cell Rep. 7: 583–586

  29. Sciaky D, Montoya AL, Chilton M-D (1978) Plasmid 1: 238–253

  30. Snowden K (1991) MSc thesis, University of Auckland, New Zealand

  31. Taylor B, Powell A (1983) Focus 4: 4–6

  32. Uematsu C, Murase M, Ichikawa H, Imamura J (1991) Plant Cell Rep. 10: 286–290

  33. Van Wordragen MF, De Jong J, Schornagel MJ, Dons HJM (1992) Plant Science 81: 207–214

  34. Veluthambi K, Krishnan M, Gould JH, Smith RH, Gelvin SB (1989) J. Bacteriol. 171: 3696–3703

  35. White FF, Nester EW (1980) J. Bacteriol. 144: 710–720

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Communicated by J. M. Widholm

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Janssen, B., Gardner, R.C. The use of transient GUS expression to develop an Agrobacterium-mediated gene transfer system for kiwifruit. Plant Cell Reports 13, 28–31 (1993).

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  • Filter Paper
  • Gene Transfer
  • Binary Vector
  • Optimise System
  • Leaf Explants