Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Ultrastructural localization of concanavalin A-binding sites in satellite cells of human skeletal muscle

  • 31 Accesses

  • 1 Citations


Electron-microscopic cytochemical studies on satellite cells of normal human skeletal muscle were carried out using the concanavalin Aperoxidase (Con A-HRP) coupling method. Con A-binding sites, which probably correspond to glycoproteins, were found to be associated with the cell surface, smooth surfaced vesicles, nuclear envelope and endoplasmic reticulum of the satellite cells and were also identified at the cell surface of the adjacent muscle fiber. The possible relationships of these observations to the functions of satellite cells are discussed.

This is a preview of subscription content, log in to check access.


  1. Agrawal BBL, Goldstein IJ (1967) Protein-carbohydrate interaction. VI. Isolation of concanavalin A by specific adsorption on cross-linked dextran gels. Biochim Biophys Acta 147:262–271

  2. Bennett G (1970) Migration of glycoprotein from Golgi apparatus to cell coat in the columnar cells of the duodenal epithelium. J Cell Biol 45:668–673

  3. Bennett G, Leblond CP, Haddad A (1974) Migration of glycoprotein from the Golgi apparatus to the surface of various cell types as shown by radioautography after labeled fucose injection into rats. J Cell Biol 60:258–284

  4. Bernhard W, Avrameas S (1971) Ultrastructural visualization of cellular carbohydrate compounds by means of concanavalin A. Exp Cell Res 64:232–236

  5. Bonilla E, Schotland DL, Wakayama Y (1978) Duchenne dystrophy: Focal alterations in the distribution of concanavalin A binding sites at the muscle cell surface. Ann Neurol 4:117–123

  6. Bretton R, Bariety J (1974) Ultrastructural localization of concanavalin A in normal rat kidney glomeruli and arterioles. J Ultrastruct Res 48:396–403

  7. Carlson BM (1973) The regeneration of skeletal muscle. A review. Am J Anat 137:119–150

  8. Fambrough DM, Devreotes PN (1978) Newly synthesized acetylcholine receptors are located in the Golgi apparatus. J Cell Biol 76:237–244

  9. Goldstein IJ, Hollerman CE, Merrick JM (1965) Protein-carbohydrate interaction. I. The interaction of polysaccharides with concanavalin A. Biochim Biophys Acta 97:68–76

  10. Gonatas NK, Avrameas S (1973) Detection of plasma membrane carbohydrates with lectin peroxidase conjugate. J Cell Biol 59:436–443

  11. Laguens R (1963) Satellite cells of skeletal muscle fibers in human progressive muscular dystrophy. Virchows Arch Pathol Anat 336:564–569

  12. Margolis RK, Gomez Z (1974) Structural changes in brain glycoproteins during development. Brain Res 74:370–372

  13. Mastiaglia FL, Papadimitriou JM, Kakulas BA (1970) Regeneration of muscle in Duchenne muscular dystrophy: An electron microscope study. J Neurol Sci 11:425–444

  14. Mauro A (1961) Satellite cell of skeletal muscle fibers. J Biophys Biochem Cytol 9:493–495

  15. McLaughlin BJ, Wood JG (1977) The localization of concanavalin A binding sites during photoreceptor synaptogenesis in the chick retina. Brain Res 119:57–71

  16. Palade G (1975) Intracellular aspects of the process of protein synthesis. Science 189:347–358

  17. Shafiq SA, Gorycki MA, Milhorat AT (1967) An electron microscopic study of regeneration and satellite cells in human muscle. Neurology 17:567–574

  18. Sumner JB, Howell SF (1966) The role of divalent metals in the reversible inactivation of jack bean hemagglutinin. J Biol Chem 115:583–588

  19. Wakayama Y (1976) Electron microscopic study on the satellite cell in the muscle of Duchenne muscular dystrophy. J Neuropathol Exp Neurol 35:532–540

  20. Wakayama Y, Schotland DL, Bonilla E, Orecchio E (1979) Quantitative ultrastructural study of muscle satellite cell in Duchenne dystrophy. Neurology 29:401–407

  21. Winzler RJ (1970) Carbohydrates in cell surfaces. Int Rev Cytol 29:77–125

  22. Wood JG, McLaughlin BJ, Barber RP (1974) The visualization of concanavalin A binding sites in Purkinje cell somata and dendrites of rat cerebellum. J Cell Biol 63:541–549

Download references

Author information

Correspondence to Eduardo Bonilla M.D..

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Wakayama, Y., Bonilla, E. & Schotland, D.L. Ultrastructural localization of concanavalin A-binding sites in satellite cells of human skeletal muscle. Cell Tissue Res. 210, 79–84 (1980). https://doi.org/10.1007/BF00232143

Download citation

Key words

  • Muscle satellite cells
  • Concanavalin A
  • Glycoproteins
  • Cell-to-cell interaction