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Agrobacterium tumefaciens mediated transformation and regeneration of muskmelon plants

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Abstract

Transgenic muskmelon (Cucumis melo L.) plants were produced efficiently by inoculating cotyledon explants with Agrobacterium tumefaciens strain LBA4404 bearing a Ti plasmid with the NPT II gene for kanaymcin resistance. After co-cultivation for three days, expiants were transferred to melon regeneration medium with kanamycin to select for transformed tissue. Shoot regeneration occurred within 3–5 weeks; excised shoots were rooted on medium containing kanamycin before transferring to soil. Morphologically normal plants were produced in three months. Southern blot analysis confirmed that ca. 85% of the regenerated plants contained the NPT gene. Dot blot analysis and leaf callus assay of progeny of transgenic plants verified transmission of the introduced gene(s) to the next generation. Factors affecting transformation efficiency are discussed.

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Abbreviations

ABA:

abscisic acid

BAP:

6-benzylaminopurine

IAA:

indole 3 acetic acid

2,4-D:

2,4-dichlorophenoxyacetic acid

NPT II:

neomycin phosphotransferase II

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Communicated by C. Quiros

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Fang, G., Grumet, R. Agrobacterium tumefaciens mediated transformation and regeneration of muskmelon plants. Plant Cell Reports 9, 160–164 (1990). https://doi.org/10.1007/BF00232095

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Keywords

  • Transgenic Plant
  • Kanamycin
  • Melon
  • Southern Blot Analysis
  • Transformation Efficiency