Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Noise of secretagogue-induced inward currents dependent on extracellular calcium in rat mast cells

  • 23 Accesses

  • 5 Citations


We analyzed the noise of the inward currents induced by stimulation of rat peritoneal mast cells with compound 48/80 (48/80), a secretagogue, and examined the role of extracellular Ca2+ in generation of the large noise. In the presence of 2 mm Ca2+ in the external solution, the power density spectra of the 48/80-induced inward currents in most cells were fitted with the sum of two Lorentzian functions. The cut-off frequencies (f c) at −50 mV for the low and high frequency components were 16.3 ±7.3 (n = 10) and 180±95 (n = 9) Hz. Involvement of a cationselective channel in the large noise was identified in some cells, but the single channel current amplitude estimated from parameters of the noise varied among cells (0.20–2.47 pA at −50 mV), thereby indicating that the currents were mediated by more than two classes of channel. The low frequency component of the 48/80-induced currents was suppressed by lowering the extracellular Ca2+ concentration to 1 μm with the addition of EGTA, without appreciable changes in the high frequency component. When the extracellular Ca2+ was reduced to 1 μm by EGTA 1 min prior to stimulation, 48/80 induced little or no currents in most cells and small currents in some cells. The power density spectra of the small currents were fitted mainly by a single Lorentzian curve with an f c of 150±5.8 Hz (n = 3). Re-admission of 1.3 mm Ca2+ produced a low frequency part of current noise with an f c of 18.8 (n = 2) Hz. When the extracellular Na+ was totally replaced by N-methyl-d-glucamine or choline in the presence of 2–5 mm Ca2+, the mean current amplitude was smaller than that in the Na+-containing medium, but the power density spectra of the current noise were fitted by a sum of two Lorentzians with f c of 13.7±6.4 (n = 6) and 186±77 (n = 6) Hz. These results suggest that low frequency fluctuation of currents depends on the extracellular Ca2+ and underlies the large noise of the 48/ 80-induced inward currents. The 48/80-induced Ca2+ influx seems to be essential to generate the low frequency fluctuations, and Na+ influx through the cation-selective channel would augment the amplitude of the fluctuation.

This is a preview of subscription content, log in to check access.


  1. Anderson, C.R., Stevens, C.F. 1973. Voltage clamp analysis of acetylcholine produced end-plate current fluctuations at frog neuromuscular junction. J. Physiol. 235:655–691

  2. Colquhoun, D., Dreyer, F., Sheridan, R. 1979. The actions of tubocurarine at the frog neuromuscular junction. J. Physiol. 293:247–284

  3. Colquhoun, D., Neher, E., Reuter, H., Stevens, C.F. 1981. Inward current channels activated by intracellular Ca2+ in cultured cardiac cells. Nature 294:752–754

  4. Cull-Candy, S.G., Howe, J.R., Ogden, D.C. 1988. Noise and single channels activated by excitatory amino acids in rat cerebellar granule neurones. J. Physiol. 400:189–222

  5. Ennis, M., Truneh, A., White, J.R., Pearce, F.L. 1980. Antigen and compound 48/80 produce a significant release of histamine in the absence of external Ca+ +. Int. Arch. Allergy Appl. Immunol. 62:467–471

  6. Fenwick, E.M., Marty, A., Neher, E. 1982. A patch clamp study of bovine chromaffin cells and of their sensitivity to acetylcholine. J. Physiol. 331:577–597

  7. Hamill, O.P., Marty, A., Neher, E., Sakmann, B., Sigworth, F.J. 1981. Improved patch-clamp techniques for high resolution current recording from cells and cell-free membrane patches. Pfluegers Arch. 391:85–100

  8. Jorissen, M., Vereecke, J., Carmeliet, E., Van-Den-Berghe, H., Cassiman, J.J. 1990. Identification of a voltage-and calciumdependent non-selective cation channel in cultured adult and fetal human nasal epithelial cells. Pfluegers Arch. 415:617–623

  9. Kuno, M., Kawaguchi, J., Mukai, M., Nakamura, F. 1990. PT pretreatment inhibits 48/80-induced activation of Ca2+-permeable channels in rat peritoneal mast cells. Am. J. Physiol. 259:C715-C722

  10. Kuno, M., Kimura, M. 1991. Effects of extracellular Ca2+ on secretagogue-induced currents in mast cells. Jpn. J. Physiol. 41:S45

  11. Kuno, M., Okada, T., Shibata, T. 1989. A patch-clamp study: Secretagogue-induced currents in rat peritoneal mast cells. Am. J. Physiol. 256:C560-C568

  12. Lindau, M., Fernandez, J.M. 1986. A patch-clamp study of histamine-secreting cells. J. Gen. Physiol. 88:349–368

  13. Marty, A., Tan, Y.P., Trautmann, A. 1984. Three types of calcium-dependent channel in rat lacrimal glands. J. Physiol. 357:293–325

  14. Matthews, G., Neher, E., Penner, R. 1989a. Second messengeractivated calcium influx in rat peritoneal mast cells. J. Physiol. 418:105–130

  15. Matthews, G., Neher, E., Penner, R. 1989b. Chloride conductance activated by external agonists and internal messengers in rat peritoneal mast cells. J. Physiol. 418:131–144

  16. Mazurek, N., Schindler, H., Schürholz, Th., Pecht, I. 1984. The cromolyn binding protein constitutes the Ca2+ channel of basophils opening upon immunological stimulus. Proc. Natl. Acad. Sci. USA 81:6841–6845

  17. Oiki, S., Okada, Y. 1987. Ca-EGTA buffer in physiological solutions. Seitai no Kagaku 38:79–83 (in Japanese)

  18. Penner, R., Matthews, G., Neher, E. 1988. Regulations of calcium influx by second messengers in rat mast cells. Nature 334:499–504

  19. Stevens, C.F. 1972. Inferences about membrane properties from electrical noise measurements. Biophys. J. 12:1028–1047

  20. Sturgess, N.C., Hales, C.N., Ashford, M.L.J. 1987. Calcium and ATP regulate the activity of a non-selective cation channel in a rat insulinoma cell line. Pfluegers Arch. 409:607–615

  21. Von-Tscharner, V., Prod'hom, B., Baggiolini, M., Reuter, H. 1986. Ion channels in human neutrophils activated by a rise in free cytosolic calcium concentration. Nature 324:369–372

  22. White, J.R., Ishizaka, T., Ishizaka, K., Sha'afi, R.I. 1984. Direct demonstration of increased intracellular concentration of free calcium as measured by quin-2 in stimulated rat peritoneal mast cells. Proc. Natl. Acad. Sci. USA 81:3978–3982

Download references

Author information

Additional information

We thank Drs. S. Matsuura and C. Edwards for critical reading of the manuscript and Ms. J. Kawawaki for technical assistance. We also thank Drs. R. Shingai, Y. Ebina and H. Yawo for helpful advice on noise analysis. This work was supported by grants from The Ministry of Education, Science and Culture, Japan, and partly by The Mochida Memorial Foundation for Medical and Pharmaceutical Research and The Uehara Memorial Foundation.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Kuno, M., Kimura, M. Noise of secretagogue-induced inward currents dependent on extracellular calcium in rat mast cells. J. Membarin Biol. 128, 53–61 (1992). https://doi.org/10.1007/BF00231870

Download citation

Key Words

  • receptor-operated Ca2+ influx
  • compound 48/80
  • mast cell
  • current noise
  • stimulus-secretion coupling