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Transformation of passionfruit (Passiflora edulis fv flavicarpa Degener.) using Agrobacterium tumefaciens

Abstract

Leaf and stem explants of passionfruit (Passiflora eadulis fv flavicarpa) were co-cultivated with a disarmed strain of Agrobacterium tunefaciens harbouring the co-integrate vector pMON200. Four plants of passionfruit were regenerated from leaf explants on agar-solidified Murashige and Skoog (1962) based medium containing 4.43 μM 6-benzyl-aminopurine and supplemented with 86 μM kanamycin sulphate. The four plants were rooted by transfer to MS based medium with 14.7 μM 3-indolebutyric acid and 2.68 μM α-naphthyleneacetic acid for 7 d, followed by MS based medium lacking growth regulators. Both media used for rooting contained 172 μM kanamycin sulphate. Rooted plants were potted and grown to maturity. Three of the plants synthesised nopaline and expressed neomycin phosphotransferase activity; DNA dot blot and polymerase chain reaction analyses confirmed the presence of the neomycin phosphotransferase gene in three plants.

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Abbreviations

BAP:

6-benzylaminopurine

CTAB:

hexadecy-Itrimethylammonium bromide

EDTA:

ethylenediaminetetraacetic acid

IBA:

3-indolebutyric acid

MS:

Murashige and Skoog (1962)

NAA:

α-naphthyleneacetic acid

NPTII:

neomycin phosphotransferase II

nptII:

neomycin phosphotransferase II gene

PCR:

polymerase chain reaction

SDS:

sodium dodecyl sulphate

Tris:

tris(hydroxymethyl)aminomethane

References

  1. Beachy RN, Loesch-Fries LS, Tumer NE (1990) Ann Rev Phytopathol 28:451–474.

  2. Cox JE, Kiely TB (1961) Agricult Gazette of New South Wales 72:314–318.

  3. d'Utra Vaz FB, Dos Santos AVP Manders G, Davey MR, Power JB (1993) Plant Cell Rep 12:220–225.

  4. Edwards K, Johnstone C, Thompson C (1991) Nuclei Acids Res 19:1349.

  5. Fillatti JJ, Sellmer J, McCown B, Haissig B, Comai L (1987) Mol Gen Genet 206:192–199.

  6. Fitsch MMM, Manshardr RM, Gonsalves D, Slightom JL (1993) Plant Cell Rep 12:245–249.

  7. Fraley RT, Rogers SG, Horsch RB, Eichholtz DA, Flick JS, Fink CL, Hoffmann NL, Sanders PR (1985) Bio/Technol 3:629–635.

  8. Fromm M, Taylor LP, Walbot V (1986) Nature 319:791–793.

  9. Golds TJ, Davey MR, Rech EL, Power JB (1990) In: Pollard JW, Walker JM (eds) Methods in Molecular Biology. Vol 6, Plant Cell and Tissue Culture. Humana Press, Clifton, New Jersey, pp 341–347.

  10. Graham J, McNicol RJ, Kumar A (1990) Plant Cell, Tissue and Org Cult 20:35–39.

  11. Hidake T, Omura M, Ogaki N, Tomiyama M, Kato A, Oshima M, Moloyoshi F (1990) Jap J Breed 40:199–207.

  12. James DJ, Uratsu S, Cheng J, Negri P, Viss P (1993) Plant Cell Rep 12:559–563.

  13. Lambert C, Tepfer D (1992) Theor Appl Genet 85:105–109.

  14. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular Cloning, A Laboratory Manual. Cold Spring Harbor Laboratory Press, New York.

  15. Mante S, Morgens PH, Scorza R, Corolts JM, Callahan AM (1991) Bio/Technol 9:853–857.

  16. Martin FW, Nakasone HY (1970) Economic Bot 24:333–340.

  17. McGranahan GH, Leslie CA, Uratsu SL, Martin LA, Dandekar AM (1988) Bio/Technol 6:800–804.

  18. Morgan AJ, Cox PN, Turner DA, Peel E, Davey MR, Gartland KMA, Mulligan BJ (1987) Plant Sci 49:37–49.

  19. Mullins MG, Tang FCA, Facciotti D (1990) Bio/Technol 8:1041–1045.

  20. Murashige T, Skoog F (1962) Physiol Plant 15:473–497.

  21. Oliveira JC (1987) In: Ruggiero C (ed) Cultura do Maracujazeiro Vol 1, Editora Legis Summa, Ribeirão Petro, pp 218–246.

  22. Patat-Ochatt EM, Ochatt SJ, Power JB (1988) J Plant Physiol 133:460–465.

  23. Pythoud F, Sinkar VP, Nester EW, Gordon MP (1987) Bio/Technol 5:1323–1327.

  24. Rougini E, Pellegrineschi A, Mencuccini D, Mariotti D (1991) Plant Cell Rep 10:291–295.

  25. Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Proc Natl Acad Sci USA 81:8014–8018.

  26. Smigocki AC, Hammerschlag FA (1991) J Amer Sci Hort Sci 116:1092–1097.

  27. Tomes DT, Ross M, Higgens R, Rao AG, Stabell M, Howard J (1990) In: Plant Molecular Biology Manual A13. Kluwer Academic Publishers, Dordrecht, The Netherlands, pp 1–22.

  28. Uematsu C, Murase M, Ichikawa H, Imamura J (1991) Plant Cell Rep 10:286–290.

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Communicated by H. Lörz

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Manders, G., Otoni, W.C., d'Utra Vaz, F.B. et al. Transformation of passionfruit (Passiflora edulis fv flavicarpa Degener.) using Agrobacterium tumefaciens . Plant Cell Reports 13, 697–702 (1994). https://doi.org/10.1007/BF00231627

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Keywords

  • Growth Regulator
  • Neomycin
  • Agrobacterium Tumefaciens
  • Polymerase Chain Reaction Analysis
  • Leaf Explants