Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Protein variation accompanies leaf dedifferentiation in sugarcane (Sacchamm officinarum) and is influenced by genotype

  • 27 Accesses

  • 5 Citations


Tissue culture lines (morphogenic and nonmorphogenic) were established in five genotypes of Saccharum officinarum L. Changes in protein expression after dedifferentiation of leaf tissue into callus were investigated by two-dimensional gel electrophoresis of cellular proteins. The findings demonstrated that protein expression was regulated both quantitatively and qualitatively in all five genotypes of sugarcane. Sixty-three dedifferention-proteins were identified, of which thirty-three were common to all genotypes. The expression of the remainder was dependent on the genotype, suggesting that the genotype within the same sugarcane species plays an important role in dedifferentiation. Three of the dedifferentiation-proteins were specific to morphogenic callus lines and one to nonmorphogenic callus lines. These proteins can be useful in characterizing the biochemical, molecular, and genetic properties of cultured cells in sugarcane, and in understanding the influence of genotype in the induction of dedifferentiation-proteins and their role in morphogenesis.

This is a preview of subscription content, log in to check access.


  1. Coppens L, Dewitte D (1990) Plant Science 67:97–105.

  2. Kawata M, Harada S, Antonio B, Oono K (1992) Plant Cell Tissue and Organ Culture 28:1–10.

  3. Kyo M, Harada H (1990) Journal of Plant Physiology 136:716–722.

  4. Moreira MA, Veloso MM, Ricardo CPP, Duque-Megalhaes MC (1990) Plant Science 66:51–57.

  5. Murashige T, Skoog F (1962) Physiologia Plantarum 15:473–497.

  6. Rajyalakshmi K, Grover A, Maheshwari N, Tyagi AK, Maheshwari SC (1991) Physiologia Plantarum 82:617–623.

  7. Ramagopal S (1989) Journal of Plant Physiology 134:395–405.

  8. Ramagopal S (1990) Theoretical and Applied Genetics 79:297–304.

  9. Reynolds TL (1990) Journal of Plant Physiology 136:213–218.

Download references

Author information

Additional information

Communicated by A. Komamine

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Ramagopal, S. Protein variation accompanies leaf dedifferentiation in sugarcane (Sacchamm officinarum) and is influenced by genotype. Plant Cell Reports 13, 692–696 (1994).

Download citation

Key words

  • Tissue culture
  • morphogenesis
  • genotype
  • protein synthesis
  • molecular markers