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Transient and stable electrotransformations of intact black Mexican sweet maize cells are obtained after preplasmolysis

Summary

When interested in plant cell transformation, the cell wall is often considered as a barrier to DNA transfer, which is only overcome by wounding or wall degrading enzymes. In this work, we demonstrate that cell plasmolysis before electropulsation is an efficient approach to DNA delivery into intact plant cells. Using such a method, transient expression (β-glucuronidase and chloramphenicol acetyltransferase) and stable expression (phosphinotricin acetyltransferase) of exogenous genes are obtained in intact black Mexican sweet maize cells.

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Abbreviations

BMS cells:

Black Mexican Sweet cells

GUS:

β-glucuronidase

CAT:

chloramphenicol acetyltransferase

PAT:

Phosphinotricin acetyltransferase

MS:

Murashige and Skoog

PCV:

packed cell volume

4-MU and 4-MUG:

4-methylumbelliferone and 4-methylumbelliferyl-glucuronide

BSA:

bovine serum albumin

TTC:

triphenyl tetrazolium chloride

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Communicated by A. M. Boudet

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Sabri, N., Pelissier, B. & Teissie, J. Transient and stable electrotransformations of intact black Mexican sweet maize cells are obtained after preplasmolysis. Plant Cell Reports 15, 924–928 (1996). https://doi.org/10.1007/BF00231589

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Keywords

  • Cell Wall
  • Plant Cell
  • Chloramphenicol
  • Transient Expression
  • Stable Expression