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Medial vestibular nucleus in the guinea-pig

I. Intrinsic membrane properties in brainstem slices

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Summary

Intracellular recordings were obtained from medial vestibular nuclei neurones (MVNn) in guinea-pig brainstem slices. Two main distinct neuronal classes were encountered. Type A MVNn (32.3%) were characterized by a broad action potential followed by a deep single afterhyperpolarization, a transient A-like rectification, and a single range of firing in response to current injection. Type B MVNn (47.1%), in contrast, were distinguished by the presence of a thin action potential followed first by a fast and then by a delayed and slower afterhyperpolarization. In addition, they displayed a secondary range of firing in their response to current injection. A majority of B MVNn also had either subthreshold plateau potentials or low threshold spike bursts or a combination thereof. A third, non-homogeneous class of cells, could not be fitted into either one of the two main classes (20.6%, type C MVNn).

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References

  1. Baker R, Evinger C, McCrea RA (1981) Some thoughts about the three neurons in the vestibulo-ocular reflex. Ann NY Acad Sci 374:171–188

  2. Bilotto G, Goldberg J, Peterson BW, Wilson VJ (1982) Dynamic properties of vestibular reflexes in the decerebrate cat. Exp Brain Res 47:343–352

  3. de Waele C, Vibert N, Baudrimont M, Vidal PP (1990) NMDA receptors contribute to the resting discharge of the vestibular neurons in the normal and hemilabyrinthectomized guinea pig. Exp Brain Res 81:125–133

  4. Goldberg JM, Smith CE, Fernandez C (1984) Relation between discharge regularity and responses to externally applied galvanic currents in vestibular nerve afferents of the squirrel monkey. J Neurophysiol 51:1236–1256

  5. Goldberg JM, Highstein SM, Moschovakis AK, Fernandez C (1987) Inputs from regularly and irregularly discharging vestibular nerve afferents to secondary neurons in the vestibular nuclei of the squirrel monkey. I. An electrophysiological analysis. J Neurophysiol 58:700–718

  6. Halliwell JV (1990) K+ channels in the central nervous system. In: Cook NS (eds) Potassium channels, structure, classification, function and therapeutic potential. John Wiley & Sons, NewYork, pp 348–381

  7. Highstein SM, Goldberg JM, Moschovakis AK, Fernandez C (1987) Inputs from regularly and irregularly discharging ves- tibular nerve afferents to secondary neurons in the vestibular nuclei of the squirrel monkey. II. Correlation with output pathways of secondary neurons. J Neurophysiol 58:719–738

  8. Llinás R, Sugimori M (1980) Electrophysiological properties of in vitro Purkinje cell somata in mammalian cerebellar slices. J Physiol (Lond) 305:171–195

  9. Llinás R, Yarom Y (1981) Electrophysiology of mammalian inferior olivary neurones in vitro. Different types of voltage-dependent ionic conductances. J Physiol (Lond) 315:549–567

  10. Llinás R (1988) The intrinsic electrophysiological properties of mammalian neurons: insights into central nervous system function. Science 242:1654–1664

  11. Orlovsky GN (1972) Activity of vestibulospinal neurons during locomotion. Brain Res 46:85–98

  12. Sah P, Hestrin S, Nicoll RA (1989) Tonic activation of NMDA receptors by ambient glutamate enhances excitability of neurons. Science 246:815–818

  13. Serafin M, de Waele C, Khateb A, Vidal PP, Mühlethaler M (1991b) Medial vestibular nucleus in the guinea-pig. II. Ionic basis of the intrinsic membrane properties in brainstem slices. Exp Brain Res 84:426–433

  14. Serafin M, Mühlethaler M (1989) Electrophysiological study of vestibular nuclei neurons in vitro. Experientia 45: A13

  15. Serafin M, Vidal PP, de Waele C, Khateb A, Mühlethaler M (1989) Burst firing of medial vestibular nuclei neurones in vitro. Soc Neurosci Abstr 15:452

  16. Serafin M, Khateb A, de Waele C, Vidal PP, Mühlethaler M (1990) Low threshold calcium spikes in medial vestibular nuclei neurones in vitro: a role in the generation of the vestibular nystagmus quick phase in vivo? Exp Brain Res 82:187–190

  17. Wilson VJ, Melvill Jones G (1979) Mammalian vestibular physiology. Plenum Press, New York

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Correspondence to M. Mühlethaler.

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Serafin, M., de Waele, C., Khateb, A. et al. Medial vestibular nucleus in the guinea-pig. Exp Brain Res 84, 417–425 (1991). https://doi.org/10.1007/BF00231464

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Key words

  • Low threshold spike
  • A-like conductance
  • Bursts
  • In vitro
  • Guinea-pig