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Revision of the amino acid sequence of human heart fatty acid-binding protein

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Cardiac-type fatty acid-binding protein (cFABP) from human heart muscle of three individuals was isolated and characterized as pI 5.3-cFABP. The proteins were structurally analyzed by tryptic peptide mapping, application of plasma desorption time-of-flight mass spectrometry and amino acid sequencing. All three preparations of human heart FABP, having 132 amino acids, differed from the published sequence [Offner et al. Biochem J 251: 191–198, 1988] in position 104, where Leu is found instead of Lys, and in position 124, where Cys is found instead of Ser.

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Correspondence to Friedrich Spener.

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Börchers, T., Højrup, P., Nielsen, S.U. et al. Revision of the amino acid sequence of human heart fatty acid-binding protein. Mol Cell Biochem 98, 127–133 (1990).

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Key words

  • fatty acid-binding protein
  • mass spectrometry
  • sequence
  • human heart