Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Molecular characterization of mitochondrial DNA of different subtypes of male-sterile cytoplasms of the sugar beet Beta vulgaris L.

  • 39 Accesses

  • 22 Citations

Summary

Mitochondrial (mt) DNA from eight cytoplasmic male-sterile (cms) lines of sugar beet from different breeding stations was investigated by restriction fragment analysis and Southern hybridization. All cms lines showed similar but not identical restriction and hybridization signal patterns, readily distinguishable from those of fertile (N) cytoplasm. Digestion of the mtDNA with BamHI, EcoRI, SalI, and XhoI revealed distinct differences between the sterile lines, and six subtypes of the S cytoplasm could be distinguished. Differences between the sterile lines were confirmed by hybridization with a \(\overline{\overline {COX}} II\) gene probe revealing minor, line-specific hybridization signals. The data presented provide evidence for the existence of considerable variation within the only commercially used source of cms in the sugar beet, the Owen's type of cytoplasm.

This is a preview of subscription content, log in to check access.

References

  1. Boutin V, Pannenbecker G, Ecke W, Schewe G, Saumitou-Laprade P, Jean R, Vernet P, Michaelis G (1987) Cytoplasmic male sterility and nuclear restorer genes in a natural population of Beta maritima: genetical and molecular aspects. Theor Appl Genet 73:625–629

  2. Brears T, Lonsdale DM (1988) The sugar beet mitochondrial genome: a complex organization generated by homologous recombination. Mol Gen Genet 214:514–522

  3. Budahn H, Kärgel HJ, Melzer R, Wolowczyk B, Weihe A (1989) Anwendung der DNA-Sondentechnik zur Unterscheidung von Pflanzen mit N-(fertil) und S-(steril) Zytoplasma bei Futterrüben, Beta vulgaris L. Arch Zuechtungsforsch 19:75–78

  4. Chase CD, Pring DR (1985) Circular plasmid DNAs from mitochondria of Sorghum bicolor. Plant Mol Biol 5:303–311

  5. Dörfel P, Weihe A, Knösche R, Börner T (1989) Mitochondrial DNA of Chenopodium album L.: a comparison of leaves and suspension cultures. Curr Genet 16:375–380

  6. Duchenne M, Lejeune B, Fouillard P, Quetier F (1989) Comparison of the organization and expression of mtDNA of fertile and male-sterile sugar beet varieties. Theor Appl Genet 78:633–640

  7. Dudareva NA, Dikalova AE, Maletsky SI, Gileva IP, Salganik RI (1989) Alterations in the structure of mitochondrial DNA in Beta vulgaris L. concerned with the development of cytoplasmic male sterility. Dokl Akad Nauk SSSR 308:1255–1258

  8. Dudareva NA, Veprev SG, Popovsky AV, Maletsky SI, Gileva IP, Salganik RI (1990) High rate spontaneous reversion to cytoplasmic male sterility in sugar beet: a characterization of the mitochondrial genomes. Theor Appl Genet 79:817–824

  9. Ecke W, Michaelis G (1990) Comparison of chloroplast and mitochondrial DNA from five morphologically distinct Beta vulgaris cultivars: sugar beet, fodder beet, beet root, foliage beet, and Swiss chard. Theor Appl Genet 79:440–442

  10. Feinberg AP, Vogelstein B (1984) A technique for labelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 137:266–267

  11. Hallden C, Bryngelsson T, Bosemark NO (1988) Two new types of cytoplasmic male sterility found in wild Beta beets. Theor Appl Genet 75:561–568

  12. Hansen BM, Marcker KA (1984) DNA sequence and transcription of a DNA minicircle isolated from male-fertile sugar beet mitochondria. Nucleic Acids Res 12:4747–4756

  13. Komarnitsky IK, Samoylov AM, Red'ko VV, Peretyayko VG, Gleba YuYu (1990) Intraspecific diversity of sugar beet (Beta vulgaris) mitochondrial DNA. Theor Appl Genet 80:253–257

  14. Levings CS III, Brown GG (1989) Molecular biology of plant mitochondria. Cell 56:171–179

  15. Lonsdale DM, Brears T, Hodge TP, Melville SE, Rottmann WH (1988) The plant mitochondrial genome: homologous recombination as a mechanism for generating heterogeneity. Philos Trans R Soc Lond Ser B 319:1449–1463

  16. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor/NY

  17. Mikami T, Kishima Y, Sugiura M, Kinoshita T (1985) Organelle genome diversity in sugar beet with normal and different sources of male-sterile cytoplasms. Theor Appl Genet 71:166–171

  18. Mikami T, Harada T, Kinoshita T (1986) Heterogeneity of circular mitochondrial DNA molecules from sugar beet with normal and male-sterile cytoplasms. Curr Genet 10:695–700

  19. Owen FV (1942) Male sterility in sugar beets produced by complementary effects of cytoplasmic and Mendelian inheritance. Am J Bot 29:692 (Abstr)

  20. Owen FV (1945) Cytoplasmically inherited male sterility in sugar beet. J Agric Res 71:423–440

  21. Powling A (1981) Species of small DNA molecules found in mitochondria of sugar beet with normal and male-sterile cytoplasms. Mol Gen Genet 183:82–84

  22. Powling A (1982) Restriction endonuclease analysis of mitochondrial DNA from sugar beet with normal and male-sterile cytoplasms. Heredity 49:117–120

  23. Powling A, Ellis THN (1983) Studies on the organelle genomes of sugar beet with male-fertile and male-sterile cytoplasms. Theor Appl Genet 65:323–328

  24. Rogers SO, Bendich AI (1985) Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol Biol 5:69–76

  25. Thomas CM (1986) The nucleotide sequence and transcription of minicircle mitochondrial DNA's associated with male-fertile and cytoplasmic male-sterile lines of sugar beet. Nucleic Acids Res 14:9353–9370

  26. Weihe A, Melzer R, Börner T (1985) Zytoplasmatische männliche Sterilität und mitochondriale DNA. Arch Zuechtungsforsch 15:371–382

  27. Weihe A, Börner T, Meixner M, Melzer R, Wolowczyk B (1988) DNA-Sonden für die Identifizierung von Cytoplasmen. Potsd Forsch B57:168–171

Download references

Author information

Additional information

Communicated by G. Wenzel

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Weihe, A., Dudareva, N.A., Veprev, S.G. et al. Molecular characterization of mitochondrial DNA of different subtypes of male-sterile cytoplasms of the sugar beet Beta vulgaris L.. Theoret. Appl. Genetics 82, 11–16 (1991). https://doi.org/10.1007/BF00231271

Download citation

Key words

  • Beta vulgaris
  • Cytoplasmic male sterility
  • mtDNA
  • Restriction fragment patterns
  • Filter hybridization