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Fatty acid binding protein removes fatty acids but not phospholipids from microsomes liposomes and sonicated vesicles

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Abstract

Evidence is provided in this paper that indicates that fatty acids but not phospholipids are removed from microsomes or artificial membranes (liposomes, unilamellar vesicles) by mouse liver cytosolic preparations enriched with fatty acid binding protein (FABP). The cytosolic proteins can act as acceptors for fatty acids but not for phospholipids of microsomal origin. Direct evidence came when liposomes made of egg yolk phosphatidylcholine, containing both [14C]labeled phospholipids and [1-14C] palmitic acid were incubated with FABP. Using sonicated vesicles as fatty acid or phospholipid donors, mouse liver fatty acid binding protein was capable of binding palmitic acid but not phospholipids. These studies suggest that liver fatty acid binding protein can interact with different kinds of membranes increasing specifically the desorption of fatty acids.

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Abbreviations

FABP:

Fatty Acid Binding Protein

PC:

Phos phatidylcholine

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Author information

Correspondence to Angel Catalá.

Additional information

Fellow of the Comisión de Investigaciones Cientificas de la Provincia de Buenos Aires (CIC), Argentina

Member of Carrera de Investigador Científico, Consejo Nacional de Investigaciones Cientificas y Técnicas de la Republica Argentina (CONICET)

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Zanetti, R., Catalá, A. Fatty acid binding protein removes fatty acids but not phospholipids from microsomes liposomes and sonicated vesicles. Mol Cell Biochem 100, 1–8 (1991). https://doi.org/10.1007/BF00230804

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Key words

  • fatty acid binding protein
  • microsomes
  • liposomes
  • sonicated vesicles