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Fatty acid binding protein removes fatty acids but not phospholipids from microsomes liposomes and sonicated vesicles

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Evidence is provided in this paper that indicates that fatty acids but not phospholipids are removed from microsomes or artificial membranes (liposomes, unilamellar vesicles) by mouse liver cytosolic preparations enriched with fatty acid binding protein (FABP). The cytosolic proteins can act as acceptors for fatty acids but not for phospholipids of microsomal origin. Direct evidence came when liposomes made of egg yolk phosphatidylcholine, containing both [14C]labeled phospholipids and [1-14C] palmitic acid were incubated with FABP. Using sonicated vesicles as fatty acid or phospholipid donors, mouse liver fatty acid binding protein was capable of binding palmitic acid but not phospholipids. These studies suggest that liver fatty acid binding protein can interact with different kinds of membranes increasing specifically the desorption of fatty acids.

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Fatty Acid Binding Protein


Phos phatidylcholine


  1. 1.

    Zilversmit DB: Lipid transfer proteins. J Lipid Res 25: 1563–1569, 1984

  2. 2.

    Ockner RK, Manning JA, Poppenhausen RB, Ho WKL: A binding protein for fatty acids in cytosol of intestinal mucosa, liver, myocardium, and other tissues. Science, Wash. 177: 56–58, 1972

  3. 3.

    Mishkin S, Stein L, Gatmaitan Z, Arias IM: The binding of fatty acids to cytoplasmic proteins: binding to Z protein in liver and other tissues of the rat. Biochem Biophys Res Commun 47: 997–1003, 1972

  4. 4.

    Rüstow B, Risse S, Kunze D: Endogenes lipidmaster, organ verteilung und diatbeeinflussung einer fettsaurebindenden proteinfraktion des leberzytosols der ratte. Acta Biol Med Get 41: 439–445, 1982

  5. 5.

    Burnett DA, Lysenko N, Manning JA, Ockner RK: Utilization of long chain fatty acids by rat liver: studies of the role of fatty acid-binding protein. Gastroenterology 77: 241–249, 1979

  6. 6.

    Takahashi K, Odani S, Ono T: Isolation and characterization of the three fractions (DE-I, DE-II and DE-III) of rat liver Z-protein and the complete primary structure of DE-II Eur J Biochem 136: 589–601, 1983

  7. 7.

    Ockner RK, Manning JA, Kane JP: Fatty acid binding protein. Isolation from rat liver, characterization and immuno-chemical quantification. J Biol Chem 257: 7872–7878, 1982

  8. 8.

    Burrier RE, Brecher P: Binding of lysophosphatidylcholine to the rat liver fatty acid binding protein. Biochim Biophys Acta 879: 229–239, 1986

  9. 9.

    Zanetti R, Catala A: Lipid binding properties of fatty acid binding protein isolated from mouse liver. Acta Physiol Pharmacol Latinoam 38: 135–144, 1988

  10. 10.

    Tangen O, Jonsson J, Orrenius S: Isolation of rat liver microsomes by gel filtration. Analyt Biochem 54: 597–603, 1973

  11. 11.

    Folch J, Lees M, Sloane Stanley GH: A simple method for the isolation and purification of total lipids from animal tissues. J Biol Chem 226: 497–509, 1957

  12. 12.

    Rusiñol A, Salomón RA, Bloj B: Phospholipid transfer activities in toad oocytes and developing embrios. J Lipid Res 28: 100–107, 1987

  13. 13.

    Chen PS Jr, Toribana TY, Warner H: Microdetermination of phosphorus. Analyt Chem 28: 1756–1758, 1956

  14. 14.

    Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275, 1951

  15. 15.

    Swetser DA, Heuckeroth RO, Gordon JI: The metabolic significance of mammalian fatty-acid-binding proteins: Abundant proteins in search of a function. Ann Rev Nutr 7: 337–359, 1987

  16. 16.

    Catala A: Stearic acid desaturation in rat liver microsomes: stimulation by fatty acid binding protein. Acta Physiol Pharmacol Latinoam 36: 19–27, 1986

  17. 17.

    Brecher P, Saouaf R, Sugarman JM, Eisenberg D, LaRosa K: Fatty acid transfer between multilamellar liposomes and fatty acid binding proteins. J Biol Chem 259: 13395–13401, 1984

  18. 18.

    Mason JT, Huang C: Hydrodynamic analysis of egg phos phatidylcholine vesicles. Ann N Y Acad Sci 308: 29–49, 1978

  19. 19.

    Bangham AD, De Gier J, Greville GD: Osmotic properties and water permeability of phospholipid liquid crystals. Chem Phys lipids 1: 225–246, 1967

  20. 20.

    Glatz JFC, Veerkamps JM: Intracellular fatty acid-binding proteins. Int J Biochem 17: 13–22, 1985

  21. 21.

    Trulzsch D, Arias IM: Z protein: Isolation and characterization of multiple forms in rat liver cytosol. Arch Biochem Biophys 209: 433–440, 1981

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Correspondence to Angel Catalá.

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Fellow of the Comisión de Investigaciones Cientificas de la Provincia de Buenos Aires (CIC), Argentina

Member of Carrera de Investigador Científico, Consejo Nacional de Investigaciones Cientificas y Técnicas de la Republica Argentina (CONICET)

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Zanetti, R., Catalá, A. Fatty acid binding protein removes fatty acids but not phospholipids from microsomes liposomes and sonicated vesicles. Mol Cell Biochem 100, 1–8 (1991).

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Key words

  • fatty acid binding protein
  • microsomes
  • liposomes
  • sonicated vesicles