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Protein kinase from Mucor rouxii

Unshielding of new cyclic AMP binding sites upon dissociation of the ternary complex holoenzyme-cyclic AMP

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Summary

Cyclic AMP binding to Mucor rouxii protein kinase holoenzyme and free regulatory subunit was measured by the classical membrane filtration technique and by equilibrium dialysis. The results obtained demonstrate that the filtration method can be used without loss of any cyclic AMP binding site. Both methods unambiguously demonstrate that the number of molecules of cyclic AMP bound to the holoenzyme are half of those bound to the regulatory subunit. This result suggests that unshielding of new cyclic AMP binding sites occurs upon dissociation of the ternary complex holoenzyme-cyclic AMP.

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Moreno, S., Pastori, R. & Passeron, S. Protein kinase from Mucor rouxii . Mol Cell Biochem 52, 13–16 (1983). https://doi.org/10.1007/BF00230584

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Keywords

  • Protein Kinase
  • Binding Site
  • Membrane Filtration
  • Ternary Complex
  • Regulatory Subunit