Applying an immunocytochemical method, a localization of the protein Cry j I in the Cryptomeria japonica pollen, which is the major allergen responsible for Japanese cedar pollinosis, is investigated with the monoclonal and polyclonal antibodies produced from the protein. The protein that reacts to the polyclonal antibody localizes on the sexine, nexine, between nexine and intine layers, orbicles, cell wall of a generative cell, Golgi body and Golgi vesicles. The allergenic protein contained in the exine and orbicles of Japanese cedar pollen can diffuse or dissolve easily from there into the mucus covering of the eye and nose, causing a response in less than 1 min after exposure. Since the orbicles have a diameter of about 430 nm, they can pass easily through the pores of most protective masks to reach the sensitive tissues of the patient. The proteins react to the monoclonal antibodies (J1BO1 and J1BO7) and localize on the Golgi body, sexine, nexine and orbicles (but not between the nexine and intine layers), and on the generative cell wall. In the young pollen grain, numerous allergenic protein particles contained in the orbicles and sexine layer, but there is only a small amount of the protein between the nexine and intine layers, since the intine layer is not yet complete at this stage. More will be accumulated there during developmental maturation. The allergenic protein is also found on the tapetal materials remaining in the young anther. Since the materials forming the exine layer and orbicles come from tapetal tissue, it is assumed that some of the allergenic protein is produced in the tapetum and localized in the orbicles and pollen wall during maturation, and that the rest of the allergenic protein is produced in the Golgi body in the mature pollen grain.
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Grote M (1992) Ultrastructural morphology and allergen in birch pollen after aqueous, anhydrous-liquid, and vapor fixation technique. J Microsc Res Tech 21:242–248
Horiguchi S, Saito Y (1964) Japanese cedar pollinosis in Nikko, Japan. Allergy (Japan) 13:16–18
Maldonado CA, Saggau W, Forssmann WG (1986) Cardiodilatin-immunoreactivity in specific atrial granules of human heart revealed by the immunogold stain. Anat Embryol 173:295–298
Miki-Hirosige H, Nakamura S (1983) Incorporation of label from myoinositol-2-3H by young anther of Lilium longiflorum. Phytomorphology 32:85–94
Mizuhira V, Notoya M, Hasegawa H (1990) New tissue fixation method for cytochemistry using microwave irradiation. I. General remarks. Acta Histochem Cytochem 23:501–524
Nakamura S, Miki-Hirosige H (1985) Fine-structural study on the formation of the generative cell wall and intine-3 layer in a growing pollen grain of Lilium longiflorum. Am J Bot 72:365–375
Probert L, De Mey J, Polak JM (981) Distinct subpopulations of enteric p-type neurons contain substance P and vasoactive intestinal polypeptide. Nature 294:470–471
Saito Y (1968) Prevention and medical treatment of the pollinosis. Yûhikaku Co, Tokyo
Sasazuki T, Nishimura Y, Muto M, Ohta N (1983) HLA-Linked genes controlling immune response and disease susceptibility. Immunol Rev 70:51–75
Sasazuki T, Nishimura Y, Muto M (1984) MHC-linked immune suppression genes and their role in immunological disorders. Immunogenetics its application to clinical medicine. Academic Press, New York, pp 21–37
Shibamura K, Nishimura Y (1991) Japan cedar pollinosis on the immunogenetics. Kagaku (Tokyo) 61:93–97
Simon EW (1974) Phospholipid and plant membrane permiability. N Phytol 73:377–420
Staff IA, Taylor PE, Smith P, Singh MB, Knox RB (1990) Cellular localization of water soluble, allergenic proteins in rye-grass (Lolium perenne) pollen using monoclonal and specific IgE antibodies with immunogold probes. Histochem 22:276–290
Yasueda H, Yui Y, Shimizu T, Shida T (1983) Isolation and partial characterization of the major allergen from Japanese cedar (Cryptomeria japonica) pollen. J Allergy Clin Immunol 71:77–86
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Miki-Hirosige, H., Nakamura, S., Yasueda, H. et al. Immunocytochemical localization of the allergenic proteins in the pollen of Cryptomeria japonica . Sexual Plant Reprod 7, 95–100 (1994). https://doi.org/10.1007/BF00230577
- Allergic symptoms
- Allergic Protein
- Cryptomeria japonica
- Cry j I