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Protein expression of a self-compatible allele from Lycopersicon peruvianum: introgression and behavior in a self-incompatible background

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Abstract

Lycopersicon peruvianum (wild tomato) is a gametophytic self-incompatible (SI) species. One natural population has been shown to harbor a self-compatible (SC) allele. A stylar protein associated with the self-compatibility allele has been elucidated using SDS-PAGE. The temporal and spatial expression of this protein is presented and compared with protein expression of two SI alleles. Hybrids containing the SC and SI alleles were used in a backcrossing program to introgress the SC allele into SI backgrounds in six independent lines. Controlled pollinations and SDS-PAGE were used to identify and select classes of progeny. After four backcross generations (approximately 97% recovery of the SI backgrounds) the SC allele still confers self-fertility in lines that contain this allele, providing evidence that the mutation to SC occurred at the S-locus and that the associated protein is likely responsible.

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Rivers, B.A., Bernatzky, R. Protein expression of a self-compatible allele from Lycopersicon peruvianum: introgression and behavior in a self-incompatible background. Sexual Plant Reprod 7, 357–362 (1994). https://doi.org/10.1007/BF00230514

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Key words

  • Self incompatibility
  • Self compatibility
  • Lycopersicon peruvianum
  • S-related proteins