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Transcription induction of c-Ki-ras with the tumour promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) in normal and transformed liver cells

Abstract

Results from nuclear run-off assays show that exposure of hepatocytes and Reuber H35B hepatoma cells to the tumour promoter, 12-0-tetradecanoylphorbol-13-acetate (TPA), leads to enhanced transcription of c-Ki-ras gene. This increase in transcription in turn results in an accumulation of the functionally active c-Ki-ras message. The half life of c-Ki-ras message in both normal and transformed livers cells is not altered by TPA and is determined to be 3.5 hr. The induction of c-Ki-ras message is accompanied by an increase in the level of c-Ki-ras protein.

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Abbreviations

H-7:

1-(5-isoquinolinylsulfonyl)-2-methylpiperazine

UTP:

Uridine Triphosphate

CTP:

Cytidine Triphosphate

ATP:

Adenosine Triphosphate

GTP:

Guanosine Triphosphate

GTPase:

Guanosine Triphosphatase

SDS-PAGE:

Sodium Dodecylsulphate Polyacrylamide Gel Electrophoresis

TPA:

12-O-tetradecanoylphorbol-13-acetate

MEM:

Minimal Essential Medium

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Correspondence to Desmond Chak Yew Yeung.

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On Chan, S., Siu Chun Wong, S. & Yew Yeung, D.C. Transcription induction of c-Ki-ras with the tumour promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) in normal and transformed liver cells. Mol Cell Biochem 117, 71–79 (1992). https://doi.org/10.1007/BF00230412

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Key words

  • c-Ki-ras
  • 12-O-tetradecanoylphorbol-13-acetate (TPA)