High-molecular-weight (HMW) glutenin subunits are a particular class of wheat endosperm proteins containing a large repetitive domain flanked by two short N- and C-terminal non-repetitive regions. Deletions and insertions within the central repetitive domain has been suggested to be mainly responsible for the length variations observed for this class of proteins. Nucleotide sequence comparison of a number of HMW glutenin genes allowed the identification of small insertions or deletions within the repetitive domain. However, only indirect evidence has been produced which suggests the occurrence of substantial insertions or deletions within this region when a large variation in molecular size is present between different HMW glutenin subunits. This paper represents the first report on the molecular characterization of an unusually large insertion within the repetitive domain of a functional HMW glutenin gene. This gene is located at the Glu-D1 locus of a hexaploid wheat genotype and contains an insertion of 561 base pairs that codes for 187 amino acids corresponding to the repetitive domain of a HMW glutenin subunit encoded at the same locus. The precise location of the insertion has been identified and the molecular processes underlying such mutational events are discussed.
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Anderson OD, Greene FC, Yip RE, Halford NG, Shewry PR, Malpica-Romero J-M (1989) Nucleotide sequences of the two highmolecular-weight glutenin genes from the D-genome of a hexaploid bread wheat, Triticum aestivum L. cv Cheyenne. Nucleic Acids Res 17:461–462
Buonocore F, Hickman DR, Caporale C, Porceddu E, Lafiandra D, Tatham AS, Shewry (1996) Characterization of a novel high Mr glutenin subunit encoded by chromosome 1D of bread wheat. J Cereal Sci 23:55–60
D'Ovidio R, Tanzarella OA, Porceddu E (1992) Isolation of an alpha-type gliadin gene from Triticum durum Desf and genetic polymorphism at the Gli-2 loci. J Genet Breed 46:41–48
D'Ovidio R, Porceddu E, Lafiandra D (1994) PCR analysis of genes encoding allelic variants of high-molecular-weight glutenin subunits at the Glu-D1 locus. Theor Appl Genet 88:175–180
D'Ovidio R, Anderson OD, Masci S, Skerritt J, Porceddu E (1996) Construction of Dx5 genes modified in the repetitive domain and their expression in Escherichia coli. In: Schofield JD (ed) Wheat structure, biochemistry and functionality. The Royal Society of Chemistry, UK (in press)
D'Ovidio R, Masci S, Porceddu E (1995) Development of a set of oligonucleotide primers specific for genes at the Glu-1 locus. Theor Appl Genet 91:189–194
Harberd NP, Bartels D, Thompson RD (1986) DNA restriction-fragment variation in the gene family encoding high-molecularweight (HMW) glutenin subunits of wheat. Biochem Genet 24:579–595
Kasarda DD, King G, Kumosinski F (1994) Comparison of spiral structures in wheat high-molecular-weight glutenin subunits and elastin by molecular modeling. In: Kumosinski F, Liebman MN (eds) Molecular modeling: from virtual tools to real problems. American Chemical Society, pp 209–220
Lagudah ES, Halloran GM (1988) Phylogenetic relationships of Triticum tauschii, the D genome donor to hexaploid wheat. Theor Appl Genet 75:592–598
Margiotta B, Colaprico G, D'Ovidio R, Lafiandra D (1993) Characterization of high Mr subunits of glutenin by combined chromatographic (RP-HPLC) and electrophoresis separations and restriction fragment length polymorphism analyses of their encoding genes. I Cereal Sci 17:221–236
Payne PI (1987) Genetics of wheat storage proteins and the effect of allelic variation on bread making quality. Annu Rev Plant Physiol 38:141–153
Payne PI, Lawrence GD (1983) Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1 and Glu-D1, which code for high-molecular-weight subunits of glutenin in hexaploid wheat. Cereal Res Commun 11:29–3
Payne PI, Holt LM, Law CN (1981) Structural and genetic studies of the high-molecular-weight subunits amongst varieties of wheat (Triticum aestivum L.). Theor Appl Genet 60:229–241
Payne PI, Holt LM, Lawrence GD (1983) Detection of a novel highmolecular-weight subunit of glutenin in some Japanese hexaploid wheats. J Cereal Sci 1:3–8
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning. A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Shewry PR (1995) Plant storage proteins. Biol Rev 70:375–426
Shewry PR, Halford NG, Tatham AS (1989) The high-molecularweight subunits of wheat, barley and rye: genetics, molecular biology, chemistry and role in wheat gluten structure and functionality. Oxford Surveys Plant Mol Cell Biol 6:163–219
Shewry PR, Halford NG, Tatham AS (1992) High-molecular-weight subunits of wheat glutenin. J Cereal Sci 15:105–120
Sugiyama T, Rafalski A, Peterson D, Soll D (1985) A wheat HMW glutenin subunit gene reveals a highly repeated structure. Nucleic Acids Res 13:8729–8737
Tatham AS, Shewry PS, Miflin BJ (1984) Wheat gluten elasticity: a similar molecular basis to elastin? FEBS Lett 177:205–208
Communicated by G. E. Hart
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D'Ovidio, R., Lafiandra, D. & Porceddu, E. Identification and molecular characterization of a large insertion within the repetitive domain of a high-molecular-weight glutenin subunit gene from hexaploid wheat. Theoret. Appl. Genetics 93, 1048–1053 (1996). https://doi.org/10.1007/BF00230123
- Triticum aestivum
- HMW glutenin genes
- Unequal crossing-over
- Glu-D1 locus