Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Free radicals in myocardial injury: experimental and clinical studies

  • 21 Accesses

  • 1 Citations


The exposure of cardiac cells to OFR generated artificially, showed a marked decrease (p < 0.01) in cellular utilization of glucose alongwith a significant decrease in calcium uptake (p < 0.05). We have also provided evidence for a direct relationship of neutrophil OFR production with the extent of myocardial ischemia in patients of myocardial infarction. Our data provides evidence for implication of OFR in myocardial injury and the pivotal role played by modulators like calcium, ECGF and prostaglandins in potentiating damage to the myocardium.

This is a preview of subscription content, log in to check access.


  1. 1.

    Southorn PA, Powis G: Free radicals in medicine. Involvement in Human Disease in Mayo Clin Proc 63: 390–408, 1988

  2. 2.

    Marx JL: Oxygen Free radicals linked to many diseases Science. 235: 529–531, 1987

  3. 3.

    Fantone JC and Ward PA: Role of oxygen derived free radicals and metabolites in leukocyte dependent inflammatory reactions. Am J Pathol 107, 3: 397–418, 1982

  4. 4.

    Thompson JA and Hess ML: The oxygen free radicals system. A fundamental mechanism in the production of myocardial necrosis. Progress in Cardiovasc Dis XXVII: 449–462, 1986

  5. 5.

    Jennings RB, Reimer KA: Lethal myocardial ischemic injury. Am J Pathol 102: 282–291, 1981

  6. 6.

    McCord JM and Roy RS: The pathophysiology of superoxides role in inflammation and ischemia. Can J Physiol Pharmacol 60: 1346–1352, 1982

  7. 7.

    Jennings RB, Ganote CE: Structural changes in myocardium during acute ischemia. Circ Res 35: Supp III 156–172, 1974

  8. 8.

    Jacob HS: The role of activated complement and granulocytes in shock states and myocardial infarction. J Lab Clin Med 98: 645–653, 1981

  9. 9.

    Lucchesi BR and Mullane KM: Do leukocyte influence infarct size. In: DJ Hearse, DM Yellow (eds.) Therapeutic approaches to myocardial infarct size limitation. New York Raven Press 1984: 219–248

  10. 10.

    Romson JL, Hook BG, Kunkel SL: Reduction of the extent of ischemic myocardial injury by neutrophil depletion in the dog. 67: 1016–1023, 1983

  11. 11.

    Wahi S, Nalini K, Ganguly NK, Verma S, Sharma BK and Wahi PL: Neutrophil free oxygen production proportinates with degree of myocardial ischemia. Can J Cardiol 1991 (Accepted).

  12. 12.

    Lefter DM, Polansky ES: Beneficial effects of ibuprofen in acute myocardial ischemia. Cardiology 64: 265–279, 1979

  13. 13.

    Kazanjian PH and Pennington JE: Influence of drugs that block calcium channel on the microbicidal functions of human neutrophils. J Infect Dis 151: 15–22, 1985

  14. 14.

    Nalini K, Andrabi KI, Ganguly NK and Wahi PL: Nifedipine impairs neutrophils respiratory burst by a mechanism other than calcium channel blockade. Mol Cell Biochem 93: 27–34, 1990

  15. 15.

    John A Oates, Fitz Gerald A, Garret Branch A Robert, Jackson KE, Knapp H and Robert JL: Clinical implication of prostaglandin and Thromboxane formation. New England J Med 319: 689–767 [11]

  16. 16.

    Russel R: The pathogenesis of atherosclerosis. Heart Disease (eds.) E Braunwald publishers W.B. Saunders. Philadelphia, London, 1988, 1135–1152

  17. 17.

    Boyum A: Isolation of mononuclear cells and granulocytes from human blood. Scan J Clin Invest 21: 77–89, 1968

  18. 18.

    Cheung K, Archibald AC, Robindson MF: Luminol dependent chemiluminescence produced by PMN's stimulated by immune complex Aust J Exptl Bio Med Sci 62: 403–419, 1984

  19. 19.

    Clark MG: Isolation of rat heart cell. J Mol Cell Cardiol 1978, 1101–1120

  20. 20.

    Mc Donough KH, Henry JH and Spitzer JJ: Effects of oxygen radicals in substrate oxidation by cardiac myocytes. BBA. 926: 127–131, 1987

  21. 21.

    Babior and Cohen JH: Measurement of neutrophil functions in leukocyte functions. In: J Martin (eds.) Cline Churchill Living Stone USA, 1–38

  22. 22.

    Reeves JP and Sutko JL: Sodium calcium ion exchange in cardiac membrane vesicles. Proc Natl Acad Sci 79: 590–594, 1979

  23. 23.

    Salmon JA: A radioimmunoassay for 6-keto prostaglandin F. Prostaglandins 15: 383–398, 1978

  24. 24.

    Viikka L, Ylikorkal O: Measurement of TxB2 in human plasma or serum by RIA. Prostaglandins 15: 383–398, 1980

  25. 25.

    Chakravarti RN, Mohan AP, Komal HS: Atherosclerosis in Maccaca malata. Histopathological, morphometric and histochemical studies in aorta and coronary arteries of spontaneous and induced atherosclerosis Exp Mol Pathol 25: 390–401, 1976

  26. 26.

    Burgess WH, Mehlman T, Friesel R, Johnson WV, Maciag T: Multiple forms of endothelial cells growth factor. JBC. 260, 21: 11389–11392, 1985

  27. 27.

    Werns SW, Shea MJ, Driscoll EM: The independent effects of oxygen radical scavengers on canine infarct size. Circ Res 56: 895–899, 1985

  28. 28.

    Maridonneau IP, Harpey C: Effect of Trimetazidine on membrane damage induced by oxygen free radicals in human red cells. Br J Clin Pharmacol 20: 148–151, 1985

  29. 29.

    Dhawan V, Ganguly NK, Majumdar S and Chakravarti RN: Short term therapy of atherosclerosis with low dose indomethacin. J Med Primatol 19: 663–673, 1990

  30. 30.

    Fajardo FL: The complexity of endothelial cells. Am J Clin Pathol 92: 241–250, 1989

Download references

Author information

Correspondence to N. K. Ganguly.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Ganguly, N.K., Nalini, K., Wahi, S. et al. Free radicals in myocardial injury: experimental and clinical studies. Mol Cell Biochem 111, 71–76 (1992). https://doi.org/10.1007/BF00229576

Download citation

Key words

  • neutrophil
  • free oxygen radicals
  • calcium
  • endothelial cell growth factors
  • prostaglandins
  • indomethacin