Cerebral blood flow and histopathological changes after bilateral carotid artery ligation (BCAL) in Wistar rats were studied. Eight of the 38 rats (21%) died within one week. In the 30 survivors, the incidence of histopathological change was 90% in the caudate nucleus, 23% in the cortex, 30% in the hippocampus, and 0% in the other structures. Local cerebral blood flow (LCBF) was measured using the quantitative autoradiographic 14C-iodoantipyrine technique in 24 anatomically discrete regions of the brain. BCAL induced ischemia in the entire forebrain. The percent reduction of LCBF was between 25–94% of the control at 2.5 h after BCAL. LCBF tended to recover 1 week after BCAL except for the regions of neuronal damage. These results suggest that neuronal damage does not correlate with the flow rate. In the present study, selective neuronal damage was also observed in rats with chronic cerebral ischemia.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Busto R, Dietrich WD, Globus MY-T, Valdes I, Scheinberg P, Ginsberg MD (1987) Small differences in intraischemic brain temperature critically determine the extent of ischemic neuronal injury. J Cereb Blood Flow Metab 7:729–738
Choki J, Yamaguchi T, Takeya Y, Morotomi Y, Omae T (1977) Effect of carotid artery ligation on regional cerebral blood flow in normotensive and spontaneously hypertensive rats. Stroke 8:374–379
Eklöf B, Siesjö BK (1972) The effect of bilateral carotid ligation upon the blood flow and energy state of the rat brain. Acta Physiol Scand 86:155–165
Eklöf B, Siesjö BK (1973) Cerebral blood flow in ischemia caused by carotid artery ligation in the rat. Acta Physiol Scand 87:69–77
Fujishima M, Ogata J, Sugi T, Omae T (1976) Mortality and cerebral metabolism after bilateral carotid artery ligation in normotensive and spontaneously hypertensive rats. J Neurol Neurosurg Psychiat 39:212–217
Fujishima M, Ishitsuka T, Nakatomi Y, Tamaki K, Omae (1981) Changes in local cerebral blood flow following bilateral carotid occlusion in spontaneously hypertensive and normotensive rats. Stroke 12:874–876
Ito U, Spatz M, Walker JT, Klatzo I (1975) Experimental cerebral ischemia in mongolian gerbils. I. Light microscopic observations. Acta Neuropathol 32:209–223
Jorgensen MB, Diemer NH (1982) Selective neuron loss after cerebral ischemia in the rat: possible role of transmitter glutamate. Acta Neurol Scand 66:536–546
Kirino T (1982) Delayed neuronal death in the gerbil hippocampus following ischemia. Brain Res 39:57–69
Kirino T, Tamura A, Sano K (1986) A reversible type of neuronal injury following ischemia in the gerbil hippocampus. Stroke 17:455–459
Nördstrom C-H, Rehncrona S (1977) Postischemic cerebral blood flow and oxygen utilization rate in rats anesthetized with nitrous oxide or phenobarbital. Acta Physiol Scand 101:230–240
Payan HM, Levine S, Strebel R (1965) Effects of cerebral ischemia in various strains of rats. Proc Soc Exp Biol Med 120:208–209
Pulsinelli WA, Brierley JB, Plum F (1982) Temporal profile of neuronal damage in a model of transient forebrain ischemia. Ann Neurol 11:491–498
Rothman SM, Olney JW (1986) Glutamate and the pathophysiology of hypoxic-ischemic brain damage. Ann Neurol 19:105–111
Sakurada O, Kennedy C, Jehle J, Brown JD, Carbin GL, Sokoloff L (1978) Measurement of local cerebral blood flow with 14C-iodoantipyrine. Am J Physiol 234:H59-H66
Smith M-L, Auer RN, Siesjö BK (1984) The density and distribution of ischemic brain injury in the rat following 2–10 min of forebrain ischemia. Acta Neuropathol 64:319–332
Tomida S, Nowak TS Jr, Vass K, Lohr JM, Klatzo I (1987) Experimental model for repetitive ischemic attacks in the gerbil: the cumulative effect of repeated ischemic insults. J Cereb Blood Flow Metab 7:773–782
Umemura S, Yoshida T, Iwayama K, Mabe H, Nagai H (1982) Experimental study of ischemic rat brain — Correlation of pathological findings with rCBF. Brain and Nerve (Tokyo) 34:179–185
Waltz AG (1970) Effect of PaCO2 on blood flow and microvasculature of ischemic and nonischemic cerebral cortex. Stroke 1:27–37
About this article
Cite this article
Tsuchiya, M., Sako, K., Yura, S. et al. Cerebral blood flow and histopathological changes following permanent bilateral carotid artery ligation in Wistar rats. Exp Brain Res 89, 87–92 (1992). https://doi.org/10.1007/BF00229004
- Cerebral blood flow
- Cerebral ischemia