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Cerebral blood flow and histopathological changes following permanent bilateral carotid artery ligation in Wistar rats

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Cerebral blood flow and histopathological changes after bilateral carotid artery ligation (BCAL) in Wistar rats were studied. Eight of the 38 rats (21%) died within one week. In the 30 survivors, the incidence of histopathological change was 90% in the caudate nucleus, 23% in the cortex, 30% in the hippocampus, and 0% in the other structures. Local cerebral blood flow (LCBF) was measured using the quantitative autoradiographic 14C-iodoantipyrine technique in 24 anatomically discrete regions of the brain. BCAL induced ischemia in the entire forebrain. The percent reduction of LCBF was between 25–94% of the control at 2.5 h after BCAL. LCBF tended to recover 1 week after BCAL except for the regions of neuronal damage. These results suggest that neuronal damage does not correlate with the flow rate. In the present study, selective neuronal damage was also observed in rats with chronic cerebral ischemia.

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  1. Busto R, Dietrich WD, Globus MY-T, Valdes I, Scheinberg P, Ginsberg MD (1987) Small differences in intraischemic brain temperature critically determine the extent of ischemic neuronal injury. J Cereb Blood Flow Metab 7:729–738

  2. Choki J, Yamaguchi T, Takeya Y, Morotomi Y, Omae T (1977) Effect of carotid artery ligation on regional cerebral blood flow in normotensive and spontaneously hypertensive rats. Stroke 8:374–379

  3. Eklöf B, Siesjö BK (1972) The effect of bilateral carotid ligation upon the blood flow and energy state of the rat brain. Acta Physiol Scand 86:155–165

  4. Eklöf B, Siesjö BK (1973) Cerebral blood flow in ischemia caused by carotid artery ligation in the rat. Acta Physiol Scand 87:69–77

  5. Fujishima M, Ogata J, Sugi T, Omae T (1976) Mortality and cerebral metabolism after bilateral carotid artery ligation in normotensive and spontaneously hypertensive rats. J Neurol Neurosurg Psychiat 39:212–217

  6. Fujishima M, Ishitsuka T, Nakatomi Y, Tamaki K, Omae (1981) Changes in local cerebral blood flow following bilateral carotid occlusion in spontaneously hypertensive and normotensive rats. Stroke 12:874–876

  7. Ito U, Spatz M, Walker JT, Klatzo I (1975) Experimental cerebral ischemia in mongolian gerbils. I. Light microscopic observations. Acta Neuropathol 32:209–223

  8. Jorgensen MB, Diemer NH (1982) Selective neuron loss after cerebral ischemia in the rat: possible role of transmitter glutamate. Acta Neurol Scand 66:536–546

  9. Kirino T (1982) Delayed neuronal death in the gerbil hippocampus following ischemia. Brain Res 39:57–69

  10. Kirino T, Tamura A, Sano K (1986) A reversible type of neuronal injury following ischemia in the gerbil hippocampus. Stroke 17:455–459

  11. Nördstrom C-H, Rehncrona S (1977) Postischemic cerebral blood flow and oxygen utilization rate in rats anesthetized with nitrous oxide or phenobarbital. Acta Physiol Scand 101:230–240

  12. Payan HM, Levine S, Strebel R (1965) Effects of cerebral ischemia in various strains of rats. Proc Soc Exp Biol Med 120:208–209

  13. Pulsinelli WA, Brierley JB, Plum F (1982) Temporal profile of neuronal damage in a model of transient forebrain ischemia. Ann Neurol 11:491–498

  14. Rothman SM, Olney JW (1986) Glutamate and the pathophysiology of hypoxic-ischemic brain damage. Ann Neurol 19:105–111

  15. Sakurada O, Kennedy C, Jehle J, Brown JD, Carbin GL, Sokoloff L (1978) Measurement of local cerebral blood flow with 14C-iodoantipyrine. Am J Physiol 234:H59-H66

  16. Smith M-L, Auer RN, Siesjö BK (1984) The density and distribution of ischemic brain injury in the rat following 2–10 min of forebrain ischemia. Acta Neuropathol 64:319–332

  17. Tomida S, Nowak TS Jr, Vass K, Lohr JM, Klatzo I (1987) Experimental model for repetitive ischemic attacks in the gerbil: the cumulative effect of repeated ischemic insults. J Cereb Blood Flow Metab 7:773–782

  18. Umemura S, Yoshida T, Iwayama K, Mabe H, Nagai H (1982) Experimental study of ischemic rat brain — Correlation of pathological findings with rCBF. Brain and Nerve (Tokyo) 34:179–185

  19. Waltz AG (1970) Effect of PaCO2 on blood flow and microvasculature of ischemic and nonischemic cerebral cortex. Stroke 1:27–37

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Correspondence to Kazuhiro Sako.

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Tsuchiya, M., Sako, K., Yura, S. et al. Cerebral blood flow and histopathological changes following permanent bilateral carotid artery ligation in Wistar rats. Exp Brain Res 89, 87–92 (1992).

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Key words

  • Autoradiography
  • Cerebral blood flow
  • Cerebral ischemia
  • Iodoantipyrine
  • Rat