Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Central distributions of the efferent and afferent components of the pharyngeal branches of the vagus and glossopharyngeal nerves: an HRP study in the cat

  • 55 Accesses

  • 23 Citations

Summary

The central distributions of efferent and afferent components of the pharyngeal branches of the vagus (PH-X) and glossopharyngeal (PH-IX) nerves in the cat were studied by soaking their central cut ends in a horseradish peroxidase (HRP) solution. HRP-labelled PH-X neurones were distributed ipsilaterally in the rostral part of the nucleus ambiguus (NA) and the retrofacial nucleus (RFN); HRP-labelled PH-IX neurones were found in the ipsilateral RFN and the bulbopontine lateral reticular formation (RF). Vagal pharyngeal neurones constituted a large population of brainstem motoneurones. The population of HRP-labelled glossopharyngeal neurones was divided into two components. Indeed, on the basis of their location and somal morphology, the most ventral cells were identified as cranial motoneurones and those scattered in the lateral RF as parasympathetic preganglionic neurones. Application of HRP to the PH-IX nerve resulted also in the labelling of fibres and terminals in the alaminar spinal trigeminal nucleus and the nucleus of the solitary tract (NTS). The afferent fibres entered the lateral medulla with the glossopharyngeal roots, ran dorsomedially, then turned caudally toward the NTS and the caudal part of the alaminar spinal trigeminal motor (V) nucleus. In the NTS, labelled fibres ran mainly along the solitary tract, projecting to terminals in the dorsal and dorsolateral nuclei of the NTS.

This is a preview of subscription content, log in to check access.

References

  1. Anch AM, Remmers JE, Sauerland EK and Degroot WJ (1981) Oropharyngeal patency during waking and sleep in the Pick wickian syndrome: electromyographic activity of the tensor veli palatini. Electromyogr Clin Neurophysiol 21:317–330

  2. Andrew BL (1956) A functional analysis of the myelinited fibres of the superior laryngeal nerve of the rat. J Physiol (Lond) 133:420–432

  3. Barillot JC, Bianchi AL, Grélot L, Pio J, Roman J (1988) Le noyau ambigu chez le chat: représentation viscérotopique du pharynx. Arch Int Physiol Biochem 96:A185

  4. Berman AL (1968) The brain stem of the cat: a cytoarchitectonic atlas with stereotaxic coordinates. University of Wisconsin Press, Madison

  5. Bianchi AL, Grélot L, Iscoe S, Remmers JE (1988) Electrophysiological properties of rostral medullary respiratory neurones in the cat: an intracellular study. J Physiol (Lond) 407:293–310

  6. Bieger D, Hopkins DA (1987) Viscerotopic representation of the upper alimentary tract in the medulla oblongata in the rat: the nucleus ambiguus. J Comp Neurol 262:546–562

  7. Chibuzo GA, Cummings JF, Evans HE (1980) Autonomic innervation of the tongue: a horseradish peroxidase study in the dog. J Auton Nerv Syst 2:117–129

  8. Contreras RJ, Gomez MM, Norgren R (1980) Central origins of cranial nerve parasympathetic neurons in the rat. J Comp Neurol 190:373–394

  9. Davis PJ, Nail BS (1984) On the location and size of laryngeal motoneurons in the cat and rabbit. J Comp Neurol 230:13–32

  10. Feindel W, Hinshaw JR, Weddell G (1952) The pattern of motor innervation in mammalian striated muscle. J. Anat 86:35–48

  11. Guilleminault C, Hill MW, Simmons FB and Dement WC (1978) Obstuctive sleep apnea: electromyographic and fiberoptic studies. Exp Neurol 62:48–67

  12. Grélot L, Bianchi AL, Iscoe S, and Remmers JE (1988) Expiratory neurones of the rostral medulla: anatomical and functional correlates. Neurosci Lett 89:140–145

  13. Grélot L, Barillot JC, Bianchi AL (1989) Pharyngeal motoneurones: respiratory-related activity and responses to laryngeal afferents in the decerebrate cat. Exp Brain Res 78:336–344

  14. Hamilton RB, Norgren R (1984) Central projections of gustatory nerves in the rat. J Comp Neurol 222:560–577

  15. Hudson LC (1986) The origins of innervation of the canine caudal pharyngeal muscles: an HRP study. Brain Res 374:413–418

  16. Hwang K, Grossman MI, Ivy AC (1948) Nervous control of the cervical portion of the oesophagus. Am J Physiol 154:343–357

  17. Issa FG and Remmers JE (1989) Pathophysiology and treatment of obstructive sleep apnea. In: DH Simmon (ed) Current pulmonology. Year Book Medical Publishers Inc, Chicago, 10:327–352

  18. Kalia M, Mesulam MM (1980) Brain stem projections of sensory and motor components of the vagus complex in the cat. II. Laryngeal, tracheobronchial, pulmonary, cardiac, and gastrointestinal branches. J Comp Neurol 193:467–508

  19. Kalia M (1981) Anatomical organisation of the central respiratory neurons. Ann Rev Physiol 43:105–120

  20. Lawn AM (1966) The localization in the nucleus ambiguus of the rabbit of the cells of origin of motor nerve fibers in the glossopharyngeal nerve and various branches of the vaguus nerve by means of retrograde degeneration. J Comp Neurol 127:293–305

  21. Mesulam MM (1978) Tetramethyl benzidine for horseradish peroxidase neurohistochemistry: a non carcinogenic blue reaction-product with superior sensitivity for visualizing neural afferents and efferents. J Histochem Cytochem 26:106–117

  22. Nomura S, Mizuno N (1982) Central distribution of afferent and efferent components of the glossopharyngeal nerve: an HRP study in the cat. Brain Res 236:1–13

  23. Nonidez JF (1931) Innervation of the thyroid gland. II. Origin and course of the thyroid nerves in the dog. Am J Anat 57:135–169

  24. Sinclair WJ (1971) Role of the pharyngeal plexus in initiation of swallowing. Amer J Physiol 221:1260–1263

  25. Stuesse SL, Cruce WLR, Powell KS (1984) Organization within the cranial IX–X complex in ranid frogs: a horseradish peroxidase transport study. J Comp Neurol 222:358–365

  26. Takana Y, Yoshida Y, Hirano M, Morimoto M, Kanaseki T (1987) Intramucosal distribution of the glossopharyngeal sensory fibers of cats. Brain Res Bull 19:115–127

  27. Van Loveren H, Saunders MC, Cassini P, Keller JT (1985) Localization of motoneurons innervating the stylopharyngeus muscle in the cat. Neurosci Lett 58:251–255

  28. Yoshida Y, Miyazaki T, Hirano M, Shin T, Totoki T, Kanaseki T (1980) Location of motoneurons supplying the cricopharyngeal muscle in the cat studied by means of the horseradish peroxidase method. Neurosci Lett 18: 1–4

  29. Yoshida Y, Miyazaki T, Hirano M, Shin T, Totoki T, Kanaseki T (1981) Localization of efferent neurons innervating the pharyngeal constrictor muscles and the cervical esophagus muscle in the cat by means of the horseradish peroxidase method. Neurosci Lett 22:91–95

Download references

Author information

Correspondence to L. Grélot.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Grélot, L., Barillot, J.C. & Bianchi, A.L. Central distributions of the efferent and afferent components of the pharyngeal branches of the vagus and glossopharyngeal nerves: an HRP study in the cat. Exp Brain Res 78, 327–335 (1989). https://doi.org/10.1007/BF00228904

Download citation

Key words

  • Pharyngeal vagus nerve
  • Pharyngeal glossopharyngeal nerve
  • Nucleus ambiguus
  • Retrofacial nucleus
  • Lateral reticular formation
  • Nucleus of solitary tract
  • Alaminar spinal trigeminal nucleus
  • Horseradish peroxidase
  • Cat