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Immunocytochemical localization of α-melanocyte-stimulating hormone in the brain of the lizard, Lacerta muralis

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The distribution of α-melanocyte-stimulating hormone (α-MSH) was studied in the brain of the lizard Lacerta muralis by means of immunocytochemical staining methods. α-MSH-like containing cells were found in the ventro-lateral preoptic area and the paraventricular and supraoptic nuclei. Some scattered cells staining for α-MSH were also detected in the mesencephalo-diencephalic boundary region, while numerous α-MSH-like nerve fibres were localized in the medial eminence. No reaction was observed after the use of antiserum preabsorbed with synthetic antigen.

These findings suggest that an α-MSH-like peptidergic system could possibly be involved in the hypothalamo-hypophysial regulation and/or play a role as neurotransmitter in this animal.

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  1. Barnea A, Oliver C, Porter J (1977) Subcellular localization of α-melanocyte-stimulating hormone in the rat hypothalamus. J Neurochem 29:619–624

  2. Bons N (1983) Immunocytochemical identification of the mesotocin- and vasotocin-producing system in the brain of temperate and desert lizard species and their modifications by cold exposure. Gen Comp Endocrinol 52:56–58

  3. Bugnon C, Bloch B, Lenys D, Fellmann D (1979) Infundibular neurons of the human hypothalamus simultaneously reactive with antisera against endorphins, ACTH, MSH and β-LPH. Cell Tissue Res 199:177–196

  4. Coons AH, Leduc EH, Connolly JM (1955) Studies on antibody production. I. Method for the histochemical demonstration of specific antibody and its application to the study of the hyperimmune rabbit. J Exp Med 102:49–59

  5. Crosby EG, Showers MJ (1969) Comparative anatomy of the preoptic and hypothalamic areas. In: Haymaker W, Anderson E, Nauta WJ (eds) The hypothalamus. Thomas, Springfield, III, p 61

  6. Cruce JAF (1974) A cytoarchitectonic study of the diencephalon of the tegu lizard Tupinambis nigropunctatus. J Comp Neurol 153:215–238

  7. De Wied D (1969) Effects of peptide hormones on behavior. In: Ganong WF, Martini L (eds) Frontiers in neuroendocrinology. Oxford University Press, Oxford, p 97

  8. Doerr Schott J, Dubois MP (1977) Immunohistochemical demonstration of a SRIF-like system in the brain of the reptile: Lacerta muralis Laur. Experientia 33:947–949

  9. Doerr Schott J, Dubois MP (1978) Immunohistochemical localization of different peptidergic substances in the brain of amphibians and reptiles. In: Gaillard PJ, Boer HH (eds) Comparative endocrinology. Elsevier: Nort-Holland, Amsterdam, p 367

  10. Dubé D, Lissitzky JC, Leclerc R, Pelletier G (1978) Localization of α-melanocyte-stimulating hormone in rat brain and pituitary. Endocrinology 102:1283–1291

  11. Fasolo A, Gaudino G (1981) Somatostatin immunoreactive neurons and fibers in the hypothalamus of the newt. Gen Comp Endocrinol 43:256–263

  12. Fasolo A, Gaudino G (1982) Immunohistochemical localization of somatostatin-like immunoreactive in the hypothalamus of the lizard, Lacerta muralis. Gen Comp Endocrinol 48:205–212

  13. Gaudino G, Fasolo A (1980) Substance P-related peptides in the hypothalamus of Amphibia. Cell Tissue Res 211:241–250

  14. Goos HJT, Ligenberg PJM, Van Oordt PGWJ (1976) Immunofluorescence studies on gonadotropin releasing hormone (GRH) in the forebrain and the neurohypophysis of the green frog, Rana esculenta L. Cell Tissue Res 168:325–333

  15. Goossens N, Dierickx K, Vandesande F (1979) Immunocytochemical localization of vasotocin and mesotocin in the hypothalamus of lacertilian reptiles. Cell Tissue Res 200:223–227

  16. Goossens N, Dierickx K, Vandesande F (1980) Immunocytochemical localization of somatostatin in the brain of the lizard, Ctenosauria pectinata. Cell Tissue Res 208:499–505

  17. Guillemin R, Schally AV, Lipscomb HS, Andersen RN, Long JM (1962) On the presence in hog hypothalamus of β-corticotropin releasing factor, α- and β-melanocyte stimulating hormones, adrenocorticotropin, lysine-vasopressin and oxytocin. Endocrinology 70:471–477

  18. Hökfelt T, Johansson O, Ljungdahl A, Lundberg JM, Schultzberg M (1980) Peptidergic neurons. Nature 284:515–521

  19. Kastin AJ, Miller LH, Nockton R, Sandam CH, Schally AV, Stratton LO (1973) Behavioral aspects of melanocyte-stimulating hormone (MSH). In: Zimmerman E, Gispen WH, Marks BH, De Wied D (eds) Progress in brain research Vol 39, Elsevier, Amsterdam, p 461

  20. Krieger DT (1983) Brain peptides: what, where, and why? Science 222:975–985

  21. Oliver C, Porter JC (1978) Distribution and characterization of α-melanocyte-stimulating hormone in the rat brain. Endocrinology 102:697–705

  22. Pelletier G, Dubé D (1977) Electron microscopic immunohistochemical localization of α-MSH in the rat brain. Am J Anat 150:201–206

  23. Reaves TA Jr, Hayward JN (1979) Immunocytochemical identification of enkephalin neurons in the hypothalamic magnocellular preoptic nucleus of the goldfish, Carassius auratus. Cell Tissue Res 200:147–151

  24. Scott AP, Lowry PJ, Rees LH, Landon J (1974) Corticotropin-like peptides in the rat pituitary. J Endocrinol 61:355–367

  25. Sternberger LA (1974) Immunocytochemistry. Prentice-Hall, New Jersey, p 246

  26. Vandesande F, Dierickx K (1976) Immunocytochemical demonstration of separate vasotocinergic and mesotocinergic neurons in the amphibian hypothalamic magnocellular neurosecretory system. Cell Tissue Res 175:289–296

  27. Yui R (1983) Immunohistochemical studies on peptide neurons in the hypothalamus of the bullfrog Rana catesbeiana. Gen Comp Endocrinol 49:195–209

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Correspondence to Dr. Mauro Vallarino.

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Vallarino, M. Immunocytochemical localization of α-melanocyte-stimulating hormone in the brain of the lizard, Lacerta muralis . Cell Tissue Res. 237, 521–524 (1984). https://doi.org/10.1007/BF00228436

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Key words

  • α-Melanocyte-stimulating hormone
  • α-MSH-like peptide
  • Immunocytochemistry
  • Hypothalamus
  • Lizard (Lacerta muralis)