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Phosphoglucoisomerase-catalyzed interconversion of hexose-phosphates

Isotopic discrimination between hydrogen and tritium

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Summary

The rate of conversion of D-glucose 6-phosphate to D-fructose 6-phosphate as catalyzed by yeast phosphoglucoisomerase is about fourfold lower when 3H, rather than 1H, is present on the C2 of D-glucose 6-phosphate. This difference appears to be due mainly to a change in maximal velocity, rather than affinity. Phosphoglucoisomerase also distinguishes between 1H and 3H in terms of either their intramolecular transfer from C2 to C1 or their incorporation from water on the C1 of D-fructose 6-phosphate.

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References

  1. 1.

    Rose IA, O'Connell EL: Intramolecular hydrogen transfer in the phosphoglucose isomerase reaction. J Biol Chem 236: 3086–3092, 1961

  2. 2.

    Khym JX, Cohn WE: The separation of sugar phosphates by ion exchange with the use of the borate complex. Amer Chem Soc 75: 1153–1176, 1953

  3. 3.

    Levy J, Herchuelz A, Sener A, Malaisse Lagae F, Malaisse WJ: Cytochalasin B-induced impairment of glucose metabolism in islets of Langerhans. Endocrinology 98: 429–437, 1976

  4. 4.

    Rose IA: The use of kinetic isotope effects in the study of metabolic control. J Biol Chem 236: 603–609, 1961

  5. 5.

    Giroix M-H, Dufrane SP, Sener A, Malaisse WJ: Anomeric specificity of mammalian hexokinase. Arch Int Physiol Bioch 94: 161–172, 1986

  6. 6.

    Katz J, Rognstad R: The metabolism of glucose 2-T by adipose tissue. J Biol Chem 244: 99–106, 1969

  7. 7.

    Katz J, Wals PA, Rognstad R: Glucose phosphorylation, glucose-6-phosphatase and recycling in rat hepatocytes. J Biol Chem 253: 4530–4536, 1978

  8. 8.

    Malaisse WJ, Yihnaz MT, Malaisse-Lagae F, Sener A: Underestimation of D-glucose phosphorylation as measured by 3H2O production from D-[2-3H]glucose. Biochem Med Metab Biol 40: 35–41, 1988

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Correspondence to Willy J. Malaisse.

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Malaisse-Lagae, F., Liemans, V. & Malaisse, W.J. Phosphoglucoisomerase-catalyzed interconversion of hexose-phosphates. Mol Cell Biochem 89, 57–67 (1989). https://doi.org/10.1007/BF00228280

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Key words

  • phosphoglucoisomerase
  • isotopic discrimination