Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Use of RAPD markers to determine the genetic diversity of diploid, wheat genotypes

Summary

The genetic diversity of two diploid wheat species, Triticum monococcum and Triticum urartu (2n=2x=14), was assessed using random primers and the polymerase chain reaction (PCR). Electrophoretic analysis of the amplification products revealed a higher incidence of polymorphism in T. urartu than T. monococcum. Pair-wise comparisons of unique and shared polymorphic amplification products, were used to generate Jaccard's similarity coefficients. These were employed to construct phenograms using an unweighted pair-group method with arithmetical averages (UPGMA). The UPGMA analysis indicated a higher similarity among T. monococcum than T. urartu. Analysis of RAPD data appears to be helpful in determining the genetic relationships among genotypes.

This is a preview of subscription content, log in to check access.

References

  1. Caetano-Anolles G, Bassam BJ, Gresshoff PM (1991) DNA amplification fingerprinting using very short arbitrary oligonucleotide primers. Bio Technology 9:553–556

  2. Gale MD, Chao S, Sharp PJ (1990) RFLP mapping in wheat — progress and problems. In: Gustafson JP (ed) Gene manipulation in plant improvement II. Plenum Press, New York, pp 353–364

  3. Jaccard P (1908) Nouvelles recherches sur la distribution florale. Bull Soc Vaud Sci Nat 44:223–270

  4. Martin GB, Williams JGK, Tanksley SD (1991) Rapid identification of markers linked to a Pseudomonas resistance gene in tomato by using random primers and near-isogenic lines. Proc Natl Acad Sci USA 88:2336–2340

  5. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning a laboratory manual. Cold Spring Harbor Press, Cold Spring Harbor, NY

  6. Vavilov NI (1951) The origin, variation, immunity and breeding of cultivated plants. Chron Bot 13:1–366

  7. Vierling RA, Nguyen HT (1992) Heat-shock protein gene expression in diploid wheat genotypes differing in thermal tolerance. Crop Sci 32: 370–377

  8. Weining S, Langridge P (1991) Identification and mapping of polymorphisms in cereals based on the polymerase chain reaction. Theor Appl Genet 82:209–216

  9. Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Res 18:6531–6535

  10. Wood WI, Gitschier J, Lasky LA, Lawn RM (1985) Base-composition-independent hybridization in tetramethyl-ammonia chloride: method for oligonucleotide screening of highly complex gene libraries. Proc Natl Acad Sci USA 82:1585–1588

Download references

Author information

Additional information

Contribution of the College of Agricultural Sciences, Texas Tech University Journal No. T-4-334. This work was supported by a grant from the National Science Foundation (BSR-8552915)

Communicated by H. F. Linskens

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Vierling, R.A., Nguyen, H.T. Use of RAPD markers to determine the genetic diversity of diploid, wheat genotypes. Theoret. Appl. Genetics 84, 835–838 (1992). https://doi.org/10.1007/BF00227393

Download citation

Key words

  • RAPD
  • Genetic diversity
  • Phenograms
  • Triticum monococcum
  • Triticum urartu