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Use of RAPD markers to determine the genetic diversity of diploid, wheat genotypes


The genetic diversity of two diploid wheat species, Triticum monococcum and Triticum urartu (2n=2x=14), was assessed using random primers and the polymerase chain reaction (PCR). Electrophoretic analysis of the amplification products revealed a higher incidence of polymorphism in T. urartu than T. monococcum. Pair-wise comparisons of unique and shared polymorphic amplification products, were used to generate Jaccard's similarity coefficients. These were employed to construct phenograms using an unweighted pair-group method with arithmetical averages (UPGMA). The UPGMA analysis indicated a higher similarity among T. monococcum than T. urartu. Analysis of RAPD data appears to be helpful in determining the genetic relationships among genotypes.

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Contribution of the College of Agricultural Sciences, Texas Tech University Journal No. T-4-334. This work was supported by a grant from the National Science Foundation (BSR-8552915)

Communicated by H. F. Linskens

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Vierling, R.A., Nguyen, H.T. Use of RAPD markers to determine the genetic diversity of diploid, wheat genotypes. Theoret. Appl. Genetics 84, 835–838 (1992).

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Key words

  • RAPD
  • Genetic diversity
  • Phenograms
  • Triticum monococcum
  • Triticum urartu