The genetic diversity of two diploid wheat species, Triticum monococcum and Triticum urartu (2n=2x=14), was assessed using random primers and the polymerase chain reaction (PCR). Electrophoretic analysis of the amplification products revealed a higher incidence of polymorphism in T. urartu than T. monococcum. Pair-wise comparisons of unique and shared polymorphic amplification products, were used to generate Jaccard's similarity coefficients. These were employed to construct phenograms using an unweighted pair-group method with arithmetical averages (UPGMA). The UPGMA analysis indicated a higher similarity among T. monococcum than T. urartu. Analysis of RAPD data appears to be helpful in determining the genetic relationships among genotypes.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Caetano-Anolles G, Bassam BJ, Gresshoff PM (1991) DNA amplification fingerprinting using very short arbitrary oligonucleotide primers. Bio Technology 9:553–556
Gale MD, Chao S, Sharp PJ (1990) RFLP mapping in wheat — progress and problems. In: Gustafson JP (ed) Gene manipulation in plant improvement II. Plenum Press, New York, pp 353–364
Jaccard P (1908) Nouvelles recherches sur la distribution florale. Bull Soc Vaud Sci Nat 44:223–270
Martin GB, Williams JGK, Tanksley SD (1991) Rapid identification of markers linked to a Pseudomonas resistance gene in tomato by using random primers and near-isogenic lines. Proc Natl Acad Sci USA 88:2336–2340
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning a laboratory manual. Cold Spring Harbor Press, Cold Spring Harbor, NY
Vavilov NI (1951) The origin, variation, immunity and breeding of cultivated plants. Chron Bot 13:1–366
Vierling RA, Nguyen HT (1992) Heat-shock protein gene expression in diploid wheat genotypes differing in thermal tolerance. Crop Sci 32: 370–377
Weining S, Langridge P (1991) Identification and mapping of polymorphisms in cereals based on the polymerase chain reaction. Theor Appl Genet 82:209–216
Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acid Res 18:6531–6535
Wood WI, Gitschier J, Lasky LA, Lawn RM (1985) Base-composition-independent hybridization in tetramethyl-ammonia chloride: method for oligonucleotide screening of highly complex gene libraries. Proc Natl Acad Sci USA 82:1585–1588
Contribution of the College of Agricultural Sciences, Texas Tech University Journal No. T-4-334. This work was supported by a grant from the National Science Foundation (BSR-8552915)
Communicated by H. F. Linskens
About this article
Cite this article
Vierling, R.A., Nguyen, H.T. Use of RAPD markers to determine the genetic diversity of diploid, wheat genotypes. Theoret. Appl. Genetics 84, 835–838 (1992). https://doi.org/10.1007/BF00227393
- Genetic diversity
- Triticum monococcum
- Triticum urartu