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Amino acid transport in the rat exocrine pancreas

I. Transport of neutral amino acids and their utilization in protein synthesis

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Summary

The transport and utilization of three neutral amino acids in protein synthesis was studied using isolated pancreatic lobules incubated in vitro. The significance of the extracellular and intracellular amino acid pool in this process was analyzed removing the extracellular pool (labeled by inulin) by a cold-wash procedure. This was especially useful in short-term experiments. Double-labeling experiments indicated a predominant utilization of the intracellular amino acid pool during protein synthesis. The advantage of isolated pancreatic lobule preparations compared to tissue fragments or slices was emphasized by fine structural studies. Using freeze-etching techniques on the same preparations, differences in the distribution of membrane particles between luminal and lateral plasma membranes described earlier were confirmed, as well as the abundant occurrence of gap junctions on both membrane faces.

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References

  1. Adamson, L.F., Herington, A.C., Bornstein, L: Evidence for the selection by the membrane transport system of intracellular or extracellular amino acids for protein synthesis. Biochim. biophys. Acta (Amst.) 282, 352–365 (1972)

  2. Adamson, L.F., Ingbar, S.M.: Further studies of amino acid transport by embryonic chick bone. J. biol. Chem. 242, 2646–2652 (1967)

  3. Adler, G., Bieger, W., Peter, St., Kern, H.F.: Amino acid transport in the rat exocrine pancreas. III. Regulation of neutral amino acid transport by various doses of secretagogue. (In preparation)

  4. Alpers, D.M., Thier, S.O.: Role of the free amino acid pool of the intestine in protein synthesis. Biochim. biophys. Acta (Amst.) 262, 535–545 (1972)

  5. Begin, N., Scholefield, P.G.: The uptake of amino acids by mouse pancreas in vitro. J. biol. Chem. 240, 332–337 (1965)

  6. Bieger, W., Kern, H.F.: Studies on intracellular transport in the rat exocrine pancreas. I. Inhibition by aromatic amino acids in vitro. Virchows Arch. Abt. A, 367, 289–305 (1975)

  7. Bieger, W., Martin-Achard, A., Bassler, M., Kern, H.F.: Studies on intracellular transport of secretory proteins in the rat exocrine pancreas. IV. Stimulation by in vivo infusion of caerulein. Cell Tiss. Res. 165, 435–453 (1976)

  8. Bieger, W., Peter, St., Völkl, A., Kern, H.F.: Amino acid transport in the rat exocrine pancreas. II. Inhibition by lanthanum and tetracaine. Cell Tiss. Res. 180, 45–62 (1977)

  9. Bieger, W., Seybold, J., Kern, H.F.: Studies on intracellular transport of secretory proteins in the rat exocrine pancreas. III. Effect of cobalt, lanthanum and antimycin A. Virchows Arch. Abt. A 368, 329–345 (1975)

  10. Cheneval, J.P., Johnstone, R.M.: Changes in amino acid transport in the rat pancreas in response to fasting and feeding. Biochim. biophys. Acta (Amst.) 433, 630–637 (1976)

  11. Christensen, H.N., De Cespedes, C., Handlongten, M.E., Ronquist, G.: Energization of amino acid transport, studied for the Ehrlich ascites tumor cell. Biochim. biophys. Acta (Amst.) 300, 487–522 (1973)

  12. De Camilli, P., Peluchetti, P.D., Meldolesi, J.: Structural difference between luminal and lateral plasmalemma in pancreatic acinar cells. Nature (Lond.) 248, 245–247 (1974)

  13. Eagle, H.: Amino acid metabolism in mammalian cell cultures. Science 130, 432–437 (1959)

  14. Fern, E.B., Garlick, P.L.: The specific radioactivity of the tissue free amino acid pool as a basis for measuring the rate of protein synthesis in the rat in vivo. Biochem. J. 142, 413–419 (1974)

  15. Fern, E.B., Hider, R.C., London, E.R.: The effect of insulin on tissue pools and incorporation into protein of amino acids in rat skeletal muscle. Europ. J. clin. Invest. 1, 211–215 (1971)

  16. Friend, D.S., Gilula, N.B.: Variations in tight and gap junctions in mammalian tissues. J. Cell Biol. 53, 758–776, 1972

  17. Hider, R.C., Fern, E.B., London, E.R.: Identification in skeletal muscle of a distinct extracellular pool of amino acids, and its role in protein synthesis. Biochem. J. 121, 817–827 (1971)

  18. Ilan, J., Singer, M.: Sampling of the leucine pool from the growing peptide chain: Difference in leucine specific activity of peptidyl-transfer RNA from free and membrane-bound polysomes. J. molec. Biol. 91, 39–51 (1975)

  19. Jamieson, J.D.: Transport and discharge of exportable proteins in pancreatic exocrine cells: in vitro studies. In: Current topics in membranes and transport (F. Bronner and A. Kleinzeller, eds.), Vol. 3, pp. 273–338. New York: Academic Press, Inc. 1972

  20. Kipnis, D.M., Reiss, E., Helmreich, R.: Functional heterogeneity of the intracellular amino acid pool in mammalian cells. Biochim. biophys. Acta (Amst.) 51, 519–524 (1961)

  21. Kostyo, J.L.: Rapid effects of growth hormone on amino acid transport and protein synthesis. Ann. N.Y. Acad. Sci. 148, 389–407 (1968)

  22. Lundholm, K., Schersten, T.: Incorporation of leucine into human skeletal muscle proteins. A study of tissue amino acid pools and their role in protein biosynthesis. Acta physiol. scand. 93, 433–441 (1975)

  23. Morgan, M.E., Earl, D.C.N., Roadus, A., Wolpert, E.B., Giger, E.K., Jefferson, L.S.: Regulation of protein synthesis in heart muscle. J. biol. Chem. 246, 2152–2162 (1971)

  24. Mortimore, G.E., Woodside, K.M., Henry, J.E.: Compartmentation of free valine and its relation to protein turnover in perfused rat liver. J. biol. Chem. 247, 2776–2784 (1972)

  25. Narahara, M.T., Holloszy, J.O.: The actions of insulin, trypsin, and electrical stimulation on amino acid transport in muscle. J. biol. Chem. 249, 5435–5443 (1974)

  26. Rosenberg, L.E., Berman, M., Segal, S.: Studies on the kinetics of amino acid transport, incorporation into protein and oxidation in kidney-cortex slices. Biochim. biophys. Acta (Amst.) 71, 664–675 (1963)

  27. Samli, M.H., Lai, M.F., Barnett, C.A.: Protein synthesis in the rat anterior pituitary. I. Interrelationship of amino acid uptake to protein synthesis. Endocrinology 88, 540–547 (1971)

  28. Scheele, G.A., Palade, G.E.: Studies on the guinea pig pancreas. Parallel discharge of exocrine enzyme activities. J. biol. Chem. 250, 2660–2670 (1975)

  29. Seybold, J., Bieger, W., Kern, H.F.: Studies on intracellular transport of secretory proteins in the rat exocrine pancreas. II. Inhibition by antimicrotubular agents. Virchows Arch. Abt. A 368, 309–327 (1975)

  30. Steere, R.L.: Preparation of high-resolution freeze-etch, freeze-fracture, frozen-surface and freeze-dried replicas in a single freeze-etch module, and the use of stereo electron microscopy to obtain maximum information from them. In: Freeze-etching techniques and applications (E.L. Benedetti and P. Favard, eds.). Paris 1973

  31. Van Venrooij, W.J., Kuijper-Lenstra, A.M., Kramer, M.F.: Interrelationship between amino acid pools and protein synthesis in the rat submandibular gland. Biochim. biophys. Acta (Amst.) 312, 392–398 (1973)

  32. Van Venrooij, W.J., Poort, C., Kramer, M.F., Jansen, M.T.: Relationship between extracellular amino acids and protein synthesis in vitro in the rat pancreas. Europ. J. Biochem. 30, 427–433 (1972)

  33. Wettenhall, R.E.M., London, E.R.: Incorporation of amino acids into protein from an intracellular pool of lymphocytes. Biochim. biophys. Acta (Amst.) 390, 363–373 (1975)

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Correspondence to Prof. Dr. Horst F. Kern.

Additional information

Supported by a grant from the Deutsche Forschungsgemeinschaft, Bonn-Bad Godesberg (Ke 113/10). The expert technical assistance of Mrs. Marlis Kopp and the editorial help of Mrs. Gisela Lesch is gratefully acknowledged

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Bieger, W., Peter, S. & Kern, H.F. Amino acid transport in the rat exocrine pancreas. Cell Tissue Res. 180, 31–44 (1977). https://doi.org/10.1007/BF00227028

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Key words

  • Amino acid transport
  • Isolated pancreatic lobules
  • Protein synthesis
  • Intramembrane particles
  • Gap junctions